Mouse Angiopoietin-2 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse (rm) Angiopoietin‑1, rmAngiopoietin-3, recombinant human (rh) Angiopoietin‑2, and rhAngiopoietin-4 is observed. In Western blots, 100% cross‑reactivity with rmAngiopoietin-1 is observed and less than 10% cross‑reactivity with rhAngiopoietin-2, rhAngiopoietin-4, and rmAngiopoietin-3 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 748246
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Angiopoietin-2
    Asp68-Phe496
    Accession # O35608
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Angiopoietin‑2 by Western Blot. Western blot shows lysates of mouse placenta tissue, mouse uterus tissue, and mouse ovary tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Angiopoietin‑2 Monoclonal Antibody (Catalog # MAB7186) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin‑2 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-2

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted 66-70 kDa glycoprotein member of the angiopoietin family of growth factors. It is expressed by endothelial cells, macrophages and skeletal muscle fibers, and binds to the Tie2 receptor on select cell types, including endothelial cells and monocytes. Ang-2 has multiple functions. In the presence of growth factors, it promotes angiogenesis; in the absence of growth factors, it induces vascular regression. It is generally considered to be an antagonist of Ang-1 activity. Mature mouse Ang-2 is 478 amino acids (aa) in length (aa 19-496). It contains one coiled-coil region (aa 159-256) plus a C-terminal Fibrinogen-like domain (aa 275-495). Mouse Ang-2 undergoes covalent oligomerization, forming 140 kDa homodimers and 280 kDa homotetramers. The higher-order oligomers are strong Tie-2 agonists. Over aa 68-496, mouse Ang-2 shares 87% and 96% aa sequence identity with human and rat Ang-2, respectively.

  • Entrez Gene IDs:
    285 (Human); 11601 (Mouse)
  • Alternate Names:
    AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis
    Authors: J Retzlaff, K Thamm, CC Ghosh, W Ziegler, H Haller, SM Parikh, S David
    Sci Rep, 2017;7(0):44113.
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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