Mouse Artemin Biotinylated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Artemin in Western blots. In Western blots, less than 1% cross-reactivity with recombinant rat GDNF, recombinant human (rh) Neurturin, rhTGF-beta 1, recombinant mouse Nodal, and rhCripto-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Artemin
    Ala112-Gly224
    Accession # Q9Z0L2.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Artemin (Catalog # 1085-AR)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Artemin in Mouse Brain. Artemin was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse Artemin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1085) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Artemin
Artemin is a member of the glial cell-derived neurotrophic factor (GDNF) family of ligands, which also include GDNF, Persephin, and Neurturin. GDNF family ligands are distant members of the TGF-b superfamily. Artemin promotes the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs.
  • Entrez Gene IDs:
    9048 (Human); 11876 (Mouse)
  • Alternate Names:
    Artemin; ARTN; Enovin; EVN; EVNneurotrophic factor; NBN; Neublastin
Related Research Areas
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