Detects mouse Artemin in Western blots. In Western blots, less than 1% cross-reactivity with recombinant rat GDNF, recombinant human (rh) Neurturin, rhTGF-beta 1, recombinant mouse Nodal, and rhCripto-1 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse Artemin Ala112-Gly224 Accession # Q9Z0L2.1
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Artemin in Mouse Brain. Artemin was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse Artemin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1085) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Artemin is a member of the glial cell-derived neurotrophic factor (GDNF) family of ligands, which also include GDNF, Persephin, and Neurturin. GDNF family ligands are distant members of the TGF-b superfamily. Artemin promotes the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs.
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