Mouse Artemin Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Artemin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human GDNF and recombinant rat GDNF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Artemin
    Ala112-Gly224
    Accession # Q9Z0L2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Artemin (Catalog # 1085-AR)
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse spinal cord
  • Neutralization
    Measured by its ability to neutralize Artemin-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 15 ng/mL Recombinant Mouse Artemin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by Artemin and Neutralization by Mouse Artemin Antibody. Recombinant Mouse Artemin (Catalog # 1085-AR) stimulates proliferation in the SH‑SY5Y human neuroblastoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Artemin (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Artemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1085). The ND50 is typically 0.1-0.3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Artemin

Artemin is a member of the Glia Cell-Derived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor beta (TGF‑ beta ) superfamily (1‑4). Similar to other TGF‑ beta family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide‑linked homodimer of the carboxy-terminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands (5). Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha 1‑GFR alpha 4) and a common signaling component, cRET (receptor tyrosine kinase) (5‑8). Artemin prefers to bind to GFR alpha 3 and activites the GFR alpha 3‑RET. However, in the presence of RET, it can bind to GFR alpha 1 as well (4, 5, 9). Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs (5, 10, 11).

  • References:
    1. Lin, L-F.H. et al. (1993) Science 260:1130.
    2. Milbrandt, J. et al. (1998) Neuron 20:245.
    3. Kotzbauer, P.T. et al. (1996) Nature 384:467.
    4. Baloh, R.H. et al. (1998) Neuron 21:1291.
    5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
    6. Baloh, R.H. et al. (1997) Neuron 18:793.
    7. Jing, S. et al. (1996) Cell 85:1113.
    8. Jing, S. et al. (1997) J Biol Chem 272:33111.
    9. Nishino, J. et al. (1999) Neuron 23:725.
    10. Enomoto, H. et al. (2001) Development 128:3963.
    11. Andres, R. et al. (2001) Development 128:3685.
  • Entrez Gene IDs:
    9048 (Human); 11876 (Mouse)
  • Alternate Names:
    Artemin; ARTN; Enovin; EVN; EVNneurotrophic factor; NBN; Neublastin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
    Nature, 2016;535(7612):440-3.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Blocking
  2. The aryl hydrocarbon receptor AhR links atopic dermatitis and air pollution via induction of the neurotrophic factor artemin
    Nat. Immunol., 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice.
    Authors: Li J, Klein C, Liang C, Rauch R, Kawamura K, Hsueh AJ
    FEBS Lett., 2009;583(15):2479-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Artemin overexpression in skin enhances expression of TRPV1 and TRPA1 in cutaneous sensory neurons and leads to behavioral sensitivity to heat and cold.
    Authors: Elitt CM, McIlwrath SL, Lawson JJ, Malin SA, Molliver DC, Cornuet PK, Koerber HR, Davis BM, Albers KM
    J. Neurosci., 2006;26(33):8578-87.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
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