Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunoassay Development

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ASAH2/N-acylsphingosine Amidohydrolase-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ASAH2/N‑acylsphingosine Amidohydrolase-2
Thr34-Thr756
Accession # Q9JHE3

Specificity

Detects mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant human ASAH2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 (Catalog # 3558-AH), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse ASAH2/N‑acylsphingosine Amidohydrolase-2 (Catalog # 3558-AH)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ASAH2/N-acylsphingosine Amidohydrolase-2

The mouse ASAH2 gene encodes acylsphingosine amidohydrolase-2, also known as neutral ceramidase. Neutral ceramidase is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). A major physiological function of neutral ceramidase is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).

References

  1. Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
  2. Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
  3. Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.

Long Name

Neutral Ceramidase

Alternate Names

Nacylsphingosine Amidohydrolase2

Entrez Gene IDs

56624 (Human); 54447 (Mouse); 114104 (Rat)

Gene Symbol

ASAH2

UniProt

Q9JHE3

Additional ASAH2/N-acylsphingosine Amidohydrolase-2 Products

Product Documents for Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

For research use only

Related Research Areas

Bioactive Lipids and Receptors

Citations for Mouse ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

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