Mouse Betacellulin/BTC Biotinylated Antibody

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Product Details
Supplemental Products

Mouse Betacellulin/BTC Biotinylated Antibody Summary

Species Reactivity
Detects mouse Betacellulin/BTC in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant mouse (rm) ErbB4, rmEGF R, rmAR, recombinant human (rh) BTC, rhTGF-alpha, and rhHB-EGF is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse Betacellulin/BTC
Accession # Q543J8
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Betacellulin/BTC (Catalog # 1025-CE)
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human lung

Mouse Betacellulin/BTC Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Betacellulin/BTC Antibody (Catalog # MAB10251)

Standard: Recombinant Mouse Betacellulin Protein (Catalog # 1025-CE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Betacellulin/BTC

Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).

  1. Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805.
  2. Shing, Y. et al. (1993) Science 259:1604.
  3. Tada, H. et al. (1999) J. Cell Biochem. 72:423.
  4. Pathak, B.G. et al. (1995) Genomics 28:116.
  5. Bastian, S.E. et al. (2001) J. Endocrinol. 168:203.
  6. Li, L. et al. (2001) Endocrinology 142:5379.
Entrez Gene IDs
685 (Human); 12223 (Mouse)
Alternate Names
Betacellulin; BTC; probetacellulin

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