Mouse Betacellulin/BTC Antibody
Mouse Betacellulin/BTC Antibody Summary
Accession # Q543J8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by Betacellulin/BTC and Neutralization by Mouse Betacellulin/BTC Antibody. Recombinant Mouse Betacellulin/BTC (Catalog # 1025-CE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Betacellulin/BTC (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Betacellulin/ BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1025). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).
- Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805.
- Shing, Y. et al. (1993) Science 259:1604.
- Tada, H. et al. (1999) J. Cell Biochem. 72:423.
- Pathak, B.G. et al. (1995) Genomics 28:116.
- Bastian, S.E. et al. (2001) J. Endocrinol. 168:203.
- Li, L. et al. (2001) Endocrinology 142:5379.
Citations for Mouse Betacellulin/BTC Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Epiregulin, epigen and betacellulin antibodies and axial elongation in young guinea pigs with lens-induced myopization
Authors: L Dong, RH Zhang, WD Zhou, YF Li, HY Li, HT Wu, XH Shi, JB Jonas, WB Wei
BMC ophthalmology, 2022-04-27;22(1):193.
Species: Guinea Pig
Sample Types: In Vivo
Applications: In Vivo
Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
J Immunol, 2015-01-02;194(3):1039-46.
Sample Types: In Vivo
Applications: In Vivo
ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J
J Clin Invest, 2014-04-24;124(6):2696-708.
Sample Types: Protein
Applications: Western Blot
Betacellulin overexpression in the mouse ovary leads to MAPK3/MAPK1 hyperactivation and reduces litter size by impairing fertilization.
Authors: Gratao AA, Dahlhoff M, Sinowatz F, Wolf E, Schneider MR
Biol. Reprod., 2007-10-03;78(1):43-52.
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
Selective roles for tumor necrosis factor alpha-converting enzyme/ADAM17 in the shedding of the epidermal growth factor receptor ligand family: the juxtamembrane stalk determines cleavage efficiency.
Authors: Hinkle CL, Sunnarborg SW, Loiselle D, Parker CE, Stevenson M, Russell WE, Lee DC
J. Biol. Chem., 2004-04-05;279(23):24179-88.
Sample Types: Cell Lysates
Applications: ELISA Development, Western Blot
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