Mouse Betacellulin/BTC Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Betacellulin/BTC in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human BTC is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Betacellulin/BTC
    Asp32-Gln118
    Accession # Q543J8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Betacellulin/BTC (Catalog # 1025-CE)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed frozen sections of mouse lung and brain
  • Neutralization
    Measured by its ability to neutralize Betacellulin/BTC-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 2 ng/mL Recombinant Mouse Betacellulin/BTC.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by Betacellulin/BTC and Neutralization by Mouse Betacellulin/BTC Antibody. Recombinant Mouse Betacellulin/BTC (Catalog # 1025-CE) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Betacellulin/BTC (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Betacellulin/
BTC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1025). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Betacellulin/BTC

Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).

  • References:
    1. Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805.
    2. Shing, Y. et al. (1993) Science 259:1604.
    3. Tada, H. et al. (1999) J. Cell Biochem. 72:423.
    4. Pathak, B.G. et al. (1995) Genomics 28:116.
    5. Bastian, S.E. et al. (2001) J. Endocrinol. 168:203.
    6. Li, L. et al. (2001) Endocrinology 142:5379.
  • Entrez Gene IDs:
    685 (Human); 12223 (Mouse)
  • Alternate Names:
    Betacellulin; BTC; probetacellulin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
    Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
    J Immunol, 2015;194(3):1039-46.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  2. ZEB1 sensitizes lung adenocarcinoma to metastasis suppression by PI3K antagonism.
    Authors: Yang Y, Ahn Y, Chen Y, Tan X, Guo L, Gibbons D, Ungewiss C, Peng D, Liu X, Lin S, Thilaganathan N, Wistuba I, Rodriguez-Canales J, McLendon G, Creighton C, Kurie J
    J Clin Invest, 2014;124(6):2696-708.
    Species: Mouse
    Sample Type: Protein
    Application: WB
  3. Betacellulin overexpression in the mouse ovary leads to MAPK3/MAPK1 hyperactivation and reduces litter size by impairing fertilization.
    Authors: Gratao AA, Dahlhoff M, Sinowatz F, Wolf E, Schneider MR
    Biol. Reprod., 2007;78(1):43-52.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Selective roles for tumor necrosis factor alpha-converting enzyme/ADAM17 in the shedding of the epidermal growth factor receptor ligand family: the juxtamembrane stalk determines cleavage efficiency.
    Authors: Hinkle CL, Sunnarborg SW, Loiselle D, Parker CE, Stevenson M, Russell WE, Lee DC
    J. Biol. Chem., 2004;279(23):24179-88.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ELISA Development
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