Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all BLAME/SLAMF8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BLAME/SLAMF8
Val21-Asp231
Accession # Q9D3G2

Specificity

Detects mouse BLAME/SLAMF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human BLAME is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse BLAME/SLAMF8 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse BLAME/SLAMF8

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BLAME/SLAMF8

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of the Ig superfamily cell receptors. The 258 amino acid (aa) mature mouse BLAME contains a 211 aa extracellular domain (ECD) with an Ig V- and a C2-like domain, and a 26 aa cytoplasmic tail that lacks signaling motifs. BLAME is expressed by activated monocytes and dendritic cells. Although the function of BLAME is not known, it is likely involved in homotypic binding or heterotypic interactions with other CD2 subset members. Over the ECD region used as immunogen, mouse BLAME shares 95% and 78% aa identity with rat and human BLAME, respectively.

Long Name

SLAM Family Member 8

Alternate Names

CD353, SBBI42, SLAMF8

Entrez Gene IDs

56833 (Human); 74748 (Mouse); 289237 (Rat); 102122568 (Cynomolgus Monkey)

Gene Symbol

SLAMF8

UniProt

Q9D3G2

Additional BLAME/SLAMF8 Products

Product Documents for Mouse BLAME/SLAMF8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse BLAME/SLAMF8 Antibody

For research use only

Citations for Mouse BLAME/SLAMF8 Antibody

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