Mouse BMP-10 Antibody

Catalog #: MAB6038 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6038
MAB6038-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse BMP-10 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse BMP-10 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) BMP-10 and less than 10% cross‑reactivity with rhBMP-8a and recombinant mouse (rm) BMP‑8b is observed. No cross-reactivity with rmBMP-3, -4, -5, -6, -7, -9,
-15, rhBMP-1.1, -2, -3b, or the propeptide of rhBMP-10 is observed.

Source

Monoclonal Rat IgG_2A Clone # 654319

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP-10
Asn314-Arg421
Accession # Q9R229

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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BMP‑10 in Mouse Embryonic Heart. BMP-10 was detected in immersion fixed frozen sections of mouse embryonic heart (E13.5) using Rat Anti-Mouse BMP-10 Monoclonal Antibody (Catalog # MAB6038) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to developing myocardium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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BMP‑10 in Mouse Embryonic Heart. BMP-10 was detected in immersion fixed frozen sections of mouse embryonic heart (15 d.p.c.) using Rat Anti-Mouse BMP-10 Monoclonal Antibody (Catalog # MAB6038) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing heart. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-10

Bone morphogenetic protein 10 (BMP-10) is a TGF-beta superfamily member whose expression is restricted to the developing and postnatal heart. It is synthesized as a 424 amino acid (aa) pre-proprotein with a putative 21 aa signal peptide, a 292 aa propeptide, and a 108 aa mature protein. Mature BMP-10 is secreted as a 25 kDa disulfide-linked homodimer. The mouse BMP-10 proprotein shares 86% and 95% aa sequence identity with human and rat BMP-10, respectively.

Long Name

Bone Morphogenetic Protein 10

Entrez Gene IDs

27302 (Human); 12154 (Mouse)

Alternate Names

BMP10; BMP-10; bone morphogenetic protein 10; MGC126783