Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Transgenic Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 654319
View all BMP-10 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP-10
Asn314-Arg421
Accession # Q9R229

Specificity

Detects mouse BMP-10 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) BMP-10 and less than 10% cross‑reactivity with rhBMP-8a and recombinant mouse (rm) BMP‑8b is observed. No cross-reactivity with rmBMP-3, -4, -5, -6, -7, -9,-15, rhBMP-1.1, -2, -3b, or the propeptide of rhBMP-10 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse BMP‑10 Antibody

BMP-10 antibody in Mouse Embryonic Heart by Immunohistochemistry (IHC-Fr).

BMP‑10 in Mouse Embryonic Heart.

BMP-10 was detected in immersion fixed frozen sections of mouse embryonic heart (E13.5) using Rat Anti-Mouse BMP-10 Monoclonal Antibody (Catalog # MAB6038) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to developing myocardium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

BMP-10 antibody in Mouse Embryonic Heart by Immunohistochemistry (IHC-Fr).

BMP‑10 in Mouse Embryonic Heart.

BMP-10 was detected in immersion fixed frozen sections of mouse embryonic heart (15 d.p.c.) using Rat Anti-Mouse BMP-10 Monoclonal Antibody (Catalog # MAB6038) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing heart. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse BMP‑10 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic heart (E13.5)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-10

Bone morphogenetic protein 10 (BMP-10) is a TGF-beta superfamily member whose expression is restricted to the developing and postnatal heart. It is synthesized as a 424 amino acid (aa) pre-proprotein with a putative 21 aa signal peptide, a 292 aa propeptide, and a 108 aa mature protein. Mature BMP-10 is secreted as a 25 kDa disulfide-linked homodimer. The mouse BMP-10 proprotein shares 86% and 95% aa sequence identity with human and rat BMP-10, respectively.

Long Name

Bone Morphogenetic Protein 10

Alternate Names

BMP10

Entrez Gene IDs

27302 (Human); 12154 (Mouse)

Gene Symbol

BMP10

UniProt

Q9R229

Additional BMP-10 Products

Product Documents for Mouse BMP‑10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse BMP‑10 Antibody

For research use only

Related Research Areas

BMP Family

Citations for Mouse BMP‑10 Antibody

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Protocols

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