Mouse Carbohydrate Sulfotransferase 4/CHST4 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Carbohydrate Sulfotransferase 4/CHST4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human (rh) CHST4 is observed and less than 5% cross‑reactivity with recombinant mouse CHST5 and rhCHST6 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Carbohydrate Sulfotransferase 4/CHST4
    Arg30-Gly388
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Neutralization
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Carbohydrate Sulfotransferase 4/
CHST4 by Western Blot.

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5547) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbohydrate Sulfotransferase 4/CHST4
The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids. This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses. CHST4, also known as high endothelial cells N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L-selectin ligands. It has a catalytic preference for core 2-branched mucin-type O-glycans, but also has activity toward core 3 type of O-glycan. Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog.
  • Entrez Gene IDs:
    10164 (Human); 26887 (Mouse); 307838 (Rat)
  • Alternate Names:
    carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; Carbohydrate Sulfotransferase 4; CHST4; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.17; EC 2.8.2.21; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST2; GlcNAc6ST-2; gn6st-2; GST3; GST-3; HECGLCNAC6ST; HEC-GlcNAc6ST; HEC-GLCNAC-6-ST; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; LSSTcarbohydrate sulfotransferase 4; N-acetylglucosamine 6-O-sulfotransferase 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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