Detection of Mouse Carboxylesterase 1/|
CES1 by Western Blot. Western blot shows lysates of mouse liver. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7929) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxylesterase 1/CES1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Carboxylesterase 1/CES1 (also known as Ses-1 and Liver carboxylesterase 1) is a 60-63 kDa glycoprotein, member of a type-B carboxylesterase/lipase family localized to microsomal membrane and lumen of endoplasmic reticulum. CES1 exists as a homotrimer and homohexamer and is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES1 is expressed mostly in liver, and with lower levels found in the lung. Over amino acids (aa) 19‑565, mouse CES1 shares 75% aa identity with human CES1.
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Western Blot: Mouse Carboxylesterase 1/CES1 Antibody [AF7929]
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