Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Carboxylesterase 1/CES1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase 1/CES1
His19-Leu565
Accession # Q8VCC2

Specificity

Detects mouse Carboxylesterase 1/CES1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CES3 and recombinant human CES1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Carboxylesterase 1/CES1 Antibody

Detection of Mouse Carboxylesterase 1/ CES1 antibody by Western Blot.

Detection of Mouse Carboxylesterase 1/ CES1 by Western Blot.

Western blot shows lysates of mouse liver. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Carboxylesterase 1/CES1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7929) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carboxylesterase 1/CES1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse Carboxylesterase 1/CES1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse liver

Reviewed Applications

Read 1 review rated 5 using AF7929 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carboxylesterase 1/CES1

Carboxylesterase 1/CES1 (also known as Ses-1 and Liver carboxylesterase 1) is a 60-63 kDa glycoprotein, member of a type-B carboxylesterase/lipase family localized to microsomal membrane and  lumen of endoplasmic reticulum. CES1 exists as a homotrimer and homohexamer and is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. CES1 is expressed mostly in liver, and with lower levels found in the lung. Over amino acids (aa) 19‑565, mouse CES1 shares 75% aa identity with human CES1.   

Alternate Names

ACAT, CES1, Egasyn, HMSE1, REH, SES1, TGH

Entrez Gene IDs

1066 (Human); 12623 (Mouse); 29225 (Rat)

Gene Symbol

CES1

UniProt

Q8VCC2

Additional Carboxylesterase 1/CES1 Products

Product Documents for Mouse Carboxylesterase 1/CES1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Carboxylesterase 1/CES1 Antibody

For research use only

Citations for Mouse Carboxylesterase 1/CES1 Antibody

Customer Reviews for Mouse Carboxylesterase 1/CES1 Antibody (1)

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  • Mouse Carboxylesterase 1/CES1 Antibody
    Name: Hua Jiang
    Application: Western Blot
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 06/01/2016
    Primary ab dilution 1:2000 40 ug protein per lane
    Mouse Carboxylesterase 1/CES1 Antibody AF7929

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