Mouse Cathepsin D Biotinylated Antibody

Catalog #: BAF1029 Datasheet / COA / SDS

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BAF1029

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Mouse Cathepsin D Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Cathepsin D in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Cathepsin A, rmCathepsin B, rmCathepsin C, rmCathepsin H, and rmCathepsin X/Z/P is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin D
Ile21-Leu410
Accession # Q3UCD9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Cathepsin D (Catalog # 1029-AS)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse kidney, liver, and thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin D

Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑64), and a mature chain (residues 65‑410) (1‑3). It is expressed in most cells and overexpressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).

References

Diedrich, et al. (1990) Nucl. Acid Res. 18:7184.
Grusby, et al. (1990) Nucl. Acid Res. 18:4008.
Hetman, et al. (1994) DNA Cell Biol. 13:419.
Conner (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43.
Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
Tsukuba, et al. (2000) Mol. Cells 10:601.

Entrez Gene IDs

1509 (Human); 13033 (Mouse)

Alternate Names

cathepsin D (lysosomal aspartyl protease); Cathepsin D; CPSD; CTSD; EC 3.4.23; EC 3.4.23.5; lysosomal aspartyl peptidase; lysosomal aspartyl protease; MGC2311; neuronal 10