Mouse Cathepsin D Antibody
Mouse Cathepsin D Antibody Summary
Ile21-Leu410
Accession # Q3UCD9
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Cathepsin D by Western Blot. Western blot shows lysate of mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for Cathepsin D at approximately 28 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Cathepsin D in RAW 264.7 Mouse Cell Line. Cathepsin D was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Cathepsin D in Mouse Intestine. Cathepsin D was detected in perfusion fixed frozen sections of mouse intestine using 15 µg/mL Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to epithelial cells in intestinal villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cathepsin D
Cathepsin D is a lysosomal aspartic protease of the pepsin family (4). Mouse Cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 1-20), a propeptide (residues 21-64), and a mature chain (residues 65-410) (1-3). It is expressed in most cells and over-expressed in breast cancer cells (5). It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that Cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis (6). Cathepsin D secreted from human prostate carcinoma cells is responsible for the generation of angiostatin, a potent endogeneous inhibitor of angiogenesis (6).
- Diedrich, et al. (1990) Nucl. Acid Res. 18:7184.
- Grusby, et al. (1990) Nucl. Acid Res. 18:4008.
- Hetman, et al. (1994) DNA Cell Biol. 13:419.
- Conner, (2004) in Handbook of Proteolytic Enzymes (Barrett, et al. eds) Elsevier Academic Press, San Diego, p. 43.
- Rochefort, et al. (2000) Clin. Chim. Acta. 291:157.
- Tsukuba, et al. (2000) Mol. Cells 10:601.
Product Datasheets
Citations for Mouse Cathepsin D Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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BORC complex specific components and Kinesin-1 mediate autophagy evasion by the autophagy-resistant Mycobacterium tuberculosis Beijing strain
Authors: J Tunganunta, P Kanjanasir, T Khumpanied, S Benjaskull, B Wongprom, T Palaga, TAP Siregar, S Borwornpin, A Chaipraser, P Palittapon, M Ponpuak
Scientific Reports, 2023;13(1):1663.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC/IF -
Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
Nature Medicine, 2022;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Direct control of lysosomal catabolic activity by mTORC1 through regulation of V-ATPase assembly
Authors: E Ratto, SR Chowdhury, NS Siefert, M Schneider, M Wittmann, D Helm, W Palm
Nature Communications, 2022;13(1):4848.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Protective effect of the tunneling nanotube-TNFAIP2/M-sec system on podocyte autophagy in diabetic nephropathy
Authors: F Barutta, S Bellini, S Kimura, K Hase, B Corbetta, A Corbelli, F Fiordaliso, S Bruno, L Biancone, A Barreca, MG Papotti, E Hirsh, M Martini, R Gambino, M Durazzo, H Ohno, G Gruden
Autophagy, 2022;0(0):1-20.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis
Authors: MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu
The Journal of Biological Chemistry, 2022;0(0):102348.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Cathepsin D interacts with adenosine A2A receptors in mouse macrophages to modulate cell surface localization and inflammatory signaling
Authors: A Skopál, T Kéki, PÁ Tóth, B Csóka, B Koscsó, ZH Német, L Antonioli, A Ivessa, F Ciruela, L Virág, G Haskó, E Kókai
The Journal of Biological Chemistry, 2022;0(0):101888.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Epithelial-derived factors induce muscularis mucosa of human induced pluripotent stem cell-derived gastric organoids
Authors: K Uehara, M Koyanagi-A, T Koide, T Itoh, T Aoi
Stem Cell Reports, 2022;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Delivering progranulin to neuronal lysosomes protects against excitotoxicity
Authors: SE Davis, JR Roth, Q Aljabi, AR Hakim, KE Savell, JJ Day, AE Arrant
The Journal of Biological Chemistry, 2021;0(0):100993.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation
Authors: K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara
Journal of Neuroinflammation, 2021;18(1):164.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
Authors: J Frew, HB Nygaard
Acta neuropathologica communications, 2021;9(1):57.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis
Authors: JH Lee, JY Kim, S Noh, H Lee, SY Lee, JY Mun, H Park, WS Chung
Nature, 2020;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Network analysis of the progranulin-deficient mouse brain proteome reveals pathogenic mechanisms shared in human frontotemporal dementia caused by GRN mutations
Authors: M Huang, E Modeste, E Dammer, P Merino, G Taylor, DM Duong, Q Deng, CJ Holler, M Gearing, D Dickson, NT Seyfried, T Kukar
Acta Neuropathol Commun, 2020;8(1):163.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Cathepsin D deficiency in mammary epithelium transiently stalls breast cancer by interference with mTORC1 signaling
Authors: S Ketterer, J Mitschke, A Ketscher, M Schlimpert, W Reichardt, N Baeuerle, ME Hess, P Metzger, M Boerries, C Peters, B Kammerer, T Brummer, F Steinberg, T Reinheckel
Nat Commun, 2020;11(1):5133.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A pharmacological chaperone improves memory by reducing A&beta and tau neuropathology in a mouse model with plaques and tangles
Authors: JG Li, J Chiu, M Ramanjulu, BE Blass, D Praticò
Mol Neurodegener, 2020;15(1):1.
Species: Mouse
Sample Types: Cell Culture Superna
Applications: Western Blot -
C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis
Authors: Q Shao, M Yang, C Liang, L Ma, W Zhang, Z Jiang, J Luo, JK Lee, C Liang, JF Chen
Autophagy, 2019;0(0):1-16.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Mice deficient in the lysosomal enzyme palmitoyl-protein thioesterase 1 (PPT1) display a complex retinal phenotype
Authors: Y Atiskova, S Bartsch, T Danyukova, E Becker, C Hagel, S Storch, U Bartsch
Sci Rep, 2019;9(1):14185.
Species: Mouse
Sample Types: Tissue Culture Supernates
Applications: Western Blot -
Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy
Authors: A Kishimoto, S Kimura, J Nio-Kobaya, H Takahashi-, AM Park, T Iwanaga
Exp. Eye Res., 2018;172(0):1-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy
Sci Adv, 2016;2(9):e1601167.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention
Sci Rep, 2016;6(0):38278.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
RAB43 facilitates cross-presentation of cell-associated antigens by CD8?+ dendritic cells
Authors: Kenneth M Murphy
J. Exp. Med., 2016;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Lysosomal protein turnover contributes to the acquisition of TGFbeta-1 induced invasive properties of mammary cancer cells.
Authors: Kern U, Wischnewski V, Biniossek M, Schilling O, Reinheckel T
Mol Cancer, 2015;14(0):39.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Renal type a intercalated cells contain albumin in organelles with aldosterone-regulated abundance.
Authors: Jensen, Thomas B, Cheema, Muhammad, Szymiczek, Agata, Damkier, Helle Ha, Praetorius, Jeppe
PLoS ONE, 2015;10(4):e0124902.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Acid sphingomyelinase modulates the autophagic process by controlling lysosomal biogenesis in Alzheimer's disease.
Authors: Lee J, Jin H, Park M, Kim B, Lee P, Nakauchi H, Carter J, He X, Schuchman E, Bae J
J Exp Med, 2014;211(8):1551-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
Authors: Lai, N Chin, Gao, Mei Hua, Tang, Eric, Tang, Ruoying, Guo, Tracy, Dalton, Nancy D, Deng, Aihua, Tang, Tong
Lab Invest, 2012;92(11):1518-26.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
Authors: Zeeuwen PL, van Vlijmen-Willems IM, Cheng T
FASEB J., 2010;24(10):3744-55.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Western Blot -
Cellular repressor of E1A-stimulated genes is a bona fide lysosomal protein which undergoes proteolytic maturation during its biosynthesis.
Authors: Schahs P, Weidinger P, Probst OC, Svoboda B, Stadlmann J, Beug H, Waerner T, Mach L
Exp. Cell Res., 2008;314(16):3036-47.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Effect of immune serum and role of individual Fcgamma receptors on the intracellular distribution and survival of Salmonella enterica serovar Typhimurium in murine macrophages.
Authors: Uppington H, Menager N, Boross P, Wood J, Sheppard M, Verbeek S, Mastroeni P
Immunology, 2006;119(2):147-58.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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