Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization, ELISA Development, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 148113
View all CCL1/I-309/TCA-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CCL1/I-309/TCA-3
Lys24-Cys92
Accession # P10146

Specificity

Detects mouse CCL1 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL2, 3, 4,5,6, 7, 8, 9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL1/I‑309/TCA‑3 Antibody

Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody.

Chemotaxis Induced by CCL1/ITCA‑3 and Neutralization by Mouse CCL1/ITCA‑3 Antibody.

Recombinant Mouse CCL1/TCA‑3 (Catalog # 845-TC) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL1/TCA‑3 (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL1/TCA‑3 Monoclonal Antibody (Catalog # MAB845). The ND50 is typically 2-10 µg/mL.

Applications for Mouse CCL1/I‑309/TCA‑3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse CCL1/I‑309/TCA‑3 (Catalog # 845-TC)

Neutralization

Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR8. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 0.01 µg/mL Recombinant Mouse CCL1/I‑309/TCA‑3.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL1/I-309/TCA-3

Mouse CCL1, also known as TCA-3, is a member of the CC beta family of chemokines. The human chemokine I-309, which shares approximately 42% amino acid (aa) sequence identity, has been assumed to be the homologue of mouse TCA-3. Mouse TCA-3 and human I-309 also share significant sequence homology in the 5' flanking region of their genes and each contain an extra pair of cysteine residues not found in most other chemokines.

CCL1 cDNA encodes a 92 aa residue precursor protein with a predicted 23 aa signal peptide that is cleaved to produce a 69 aa mature protein. The sequence of CCL1 contains one potential N-linked glycosylation site. Mouse CCL1 is found on the distal portion of mouse chromosome 11 in a cluster with MIP-1 alpha, MIP-1 beta and JE. CCL1 acts by binding to the seven transmembrane spanning G-protein-coupled receptor, CCR8. CCL1 has been shown to chemoattract T cells.

References

  1. Goya, I. et al. (1998) J. Immunol. 160:1975.
  2. Burd, P. et al. (1987) J. Immunol. 139:3126.

Alternate Names

I-309, P500, SCYA1, SCYA2, TCA-3, TCA3

Entrez Gene IDs

6346 (Human); 20290 (Mouse)

Gene Symbol

CCL1

UniProt

P10146

Additional CCL1/I-309/TCA-3 Products

Product Documents for Mouse CCL1/I‑309/TCA‑3 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CCL1/I‑309/TCA‑3 Antibody

For research use only

Citations for Mouse CCL1/I‑309/TCA‑3 Antibody

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