Mouse CCL11/Eotaxin Quantikine ELISA Kit

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MME00
Mouse CCL11/Eotaxin ELISA Standard Curve
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Product Details
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Citations (49)
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Mouse CCL11/Eotaxin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Citrate Plasma (13 uL)
Sensitivity
3 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant mouse Eotaxin.  This assay cross-reacts 0.062% with Human Eotaxin and recognizes Rat Eotaxin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse Eotaxin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Eotaxin in cell culture supernates, serum, and plasma. It contains recombinant mouse Eotaxin and antibodies raised against the recombinant protein. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse Eotaxin showed dose curves that were parallel to the standard curves obtained using the E. coli-expressed kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Eotaxin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 49 197 466 46 179 446
Standard Deviation 3.2 7.2 30 3.8 11.7 28.7
CV% 6.5 3.7 6.4 8.3 6.5 6.4

Recovery

The recovery of mouse Eotaxin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 96 82-103
Citrate Plasma (n=5) 97 81-114
EDTA Plasma (n=5) 100 83-118
Serum (n=5) 98 82-111

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse Eotaxin in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CCL11/Eotaxin ELISA Linearity

Data Examples

Mouse CCL11/Eotaxin ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL11/Eotaxin

CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.

Entrez Gene IDs:
6356 (Human); 20292 (Mouse)
Alternate Names:
C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse CCL11/Eotaxin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

49 Citations: Showing 1 - 10
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  1. Andrographolide prevented toluene diisocyanate-induced occupational asthma and aberrant airway E-cadherin distribution via p38 MAPK-dependent Nrf2 induction
    Authors: I Sulaiman, K Tan, N Mohtarrudi, JC Woei Lim, J Stanslas
    Pulm Pharmacol Ther, 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  2. Translational control of depression-like behavior via phosphorylation of eukaryotic translation initiation factor 4E
    Authors: A Aguilar-Va, N Haji, D De Gregori, E Matta-Cama, MJ Eslamizade, J Popic, V Sharma, R Cao, C Rummel, A Tanti, S Wiebe, N Nuñez, S Comai, R Nadon, G Luheshi, N Mechawar, G Turecki, JC Lacaille, G Gobbi, N Sonenberg
    Nat Commun, 2018;9(1):2459.
    Species: Mouse
    Sample Types: Serum
  3. Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Increased mitochondrial arginine metabolism supports bioenergetics in asthma
    J Clin Invest, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  5. G-protein-coupled estrogen receptor agonist suppresses airway inflammation in a mouse model of asthma through IL-10.
    Authors: Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, Kobayashi Y, Kayaba H, Kikuchi Y, Chihara J, Takeda M, Ueki S, Hirokawa M
    PLoS ONE, 2015;10(3):e0123210.
    Species: Mouse
    Sample Types: BALF
  6. Rapid transport of CCL11 across the blood-brain barrier: regional variation and importance of blood cells.
    Authors: Erickson, Michelle, Morofuji, Yoichi, Owen, Joshua B, Banks, William
    J Pharmacol Exp Ther, 2014;349(3):497-507.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Genetic vaccine for respiratory syncytial virus provides protection without disease potentiation.
    Authors: Johnson T, Rangel D, Graham B, Brough D, Gall J
    Mol Ther, 2014;22(1):196-205.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Spontaneous eosinophilic nasal inflammation in a genetically-mutant mouse: comparative study with an allergic inflammation model.
    Authors: Cho SH, Oh SY, Zhu Z, Lee J, Lane AP
    PLoS ONE, 2012;7(4):e35114.
    Species: Mouse
    Sample Types: Nasal Lavage Fluid
  9. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2012;179(1):302-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation.
    Authors: Louten J, Rankin AL, Li Y
    Int. Immunol., 2011;23(5):307-15.
    Species: Mouse
    Sample Types: BALF
  11. IL-33-activated dendritic cells are critical for allergic airway inflammation.
    Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
    Eur. J. Immunol., 2011;41(6):1675-86.
    Species: Mouse
    Sample Types: BALF
  12. Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
    Authors: Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN
    Clin. Exp. Allergy, 2011;41(10):1447-55.
    Species: Mouse
    Sample Types: BALF
  13. House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
    Authors: De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA
    Eur. Respir. J., 2010;35(6):1377-87.
    Species: Rat
    Sample Types: BALF
  14. Impact of adiponectin deficiency on pulmonary responses to acute ozone exposure in mice.
    Authors: Zhu M, Hug C, Kasahara DI
    Am. J. Respir. Cell Mol. Biol., 2010;43(4):487-97.
    Species: Mouse
    Sample Types: BALF
  15. Inhaled multiwalled carbon nanotubes potentiate airway fibrosis in murine allergic asthma.
    Authors: Ryman-Rasmussen JP, Tewksbury EW, Moss OR, Cesta MF, Wong BA, Bonner JC
    Am. J. Respir. Cell Mol. Biol., 2009;40(3):349-58.
    Species: Mouse
    Sample Types: BALF
  16. The adaptor protein CIKS/Act1 is essential for IL-25-mediated allergic airway inflammation.
    Authors: Claudio E, Sonder SU, Saret S, Carvalho G, Ramalingam TR, Wynn TA, Chariot A, Garcia-Perganeda A, Leonardi A, Paun A, Chen A, Ren NY, Wang H, Siebenlist U
    J. Immunol., 2009;182(3):1617-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice.
    Authors: Shinagawa K, Ploplis VA, Castellino FJ
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L763-70.
    Species: Mouse
    Sample Types: BALF
  18. CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation.
    Authors: Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD
    J. Immunol., 2009;182(1):623-35.
    Species: Mouse
    Sample Types: BALF
  19. Ablation of type I hypersensitivity in experimental allergic conjunctivitis by eotaxin-1/CCR3 blockade.
    Authors: Miyazaki D, Nakamura T, Ohbayashi M, Kuo CH, Komatsu N, Yakura K, Tominaga T, Inoue Y, Higashi H, Murata M, Takeda S, Fukushima A, Liu FT, Rothenberg ME, Ono SJ
    Int. Immunol., 2009;21(2):187-201.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production.
    Authors: Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD
    Inflamm. Res., 2009;58(12):845-54.
    Species: Mouse
    Sample Types: BALF
  21. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
    Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
    Vaccine, 2009;27(23):3045-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. IL-13 receptor alpha2 selectively inhibits IL-13-induced responses in the murine lung.
    Authors: Zheng T, Liu W, Oh SY, Zhu Z, Hu B, Homer RJ, Cohn L, Grusby MJ, Elias JA
    J. Immunol., 2008;180(1):522-9.
    Species: Mouse
    Sample Types: BALF
  23. Tyrosine phosphatase SHP-1 in oxidative stress and development of allergic airway inflammation.
    Authors: Cho YS, Oh SY, Zhu Z
    Am. J. Respir. Cell Mol. Biol., 2008;39(4):412-9.
    Species: Mouse
    Sample Types: BALF
  24. Distinct roles for IL-13 and IL-4 via IL-13 receptor alpha1 and the type II IL-4 receptor in asthma pathogenesis.
    Authors: Munitz A, Brandt EB, Mingler M, Finkelman FD, Rothenberg ME
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7240-5.
    Species: Mouse
    Sample Types: BALF
  25. Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein.
    Authors: Johnson TR, Rothenberg ME, Graham BS
    J. Leukoc. Biol., 2008;84(3):748-59.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
    Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
    J. Exp. Med., 2008;205(9):2075-84.
    Species: Mouse
    Sample Types: Serum
  27. Adenovirus IL-13-induced airway disease in mice: a corticosteroid-resistant model of severe asthma.
    Authors: Therien AG, Bernier V, Weicker S, Tawa P, Falgueyret JP, Mathieu MC, Honsberger J, Pomerleau V, Robichaud A, Stocco R, Dufresne L, Houshyar H, Lafleur J, Ramachandran C, O'Neill GP, Slipetz D, Tan CM
    Am. J. Respir. Cell Mol. Biol., 2008;39(1):26-35.
    Species: Mouse
    Sample Types: BALF
  28. Airway house dust extract exposures modify allergen-induced airway hypersensitivity responses by TLR4-dependent and independent pathways.
    Authors: Lam D, Ng N, Lee S, Batzer G, Horner AA
    J. Immunol., 2008;181(4):2925-32.
    Species: Mouse
    Sample Types: BALF
  29. Eosinophil-nerve interactions and neuronal plasticity in rat gut associated lymphoid tissue (GALT) in response to enteric parasitism.
    Authors: O'Brien LM, Fitzpatrick E, Baird AW, Campion DP
    J. Neuroimmunol., 2008;197(1):1-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  30. Treatment with anti-CC chemokine receptor 3 monoclonal antibody or dexamethasone inhibits the migration and differentiation of bone marrow CD34 progenitor cells in an allergic mouse model.
    Authors: Ben S, Li X, Xu F, Xu W, Li W, Wu Z, Huang H, Shi H, Shen H
    Allergy, 2008;63(9):1164-76.
    Species: Mouse
    Sample Types: Serum
  31. An eosinophil immune response characterizes the inflammatory skin disease observed in Tie-2 transgenic mice.
    Authors: Voskas D, Babichev Y, Ling LS, Alami J, Shaked Y, Kerbel RS, Ciruna B, Dumont DJ
    J. Leukoc. Biol., 2008;84(1):59-67.
    Species: Mouse
    Sample Types: Serum
  32. Critical role of the Fc receptor gamma-chain on APCs in the development of allergen-induced airway hyperresponsiveness and inflammation.
    Authors: Kitamura K, Takeda K, Koya T, Miyahara N, Kodama T, Dakhama A, Takai T, Hirano A, Tanimoto M, Harada M, Gelfand EW
    J. Immunol., 2007;178(1):480-8.
    Species: Mouse
    Sample Types: BALF
  33. A critical role for Pin1 in allergic pulmonary eosinophilia in rats.
    Authors: Esnault S, Rosenthal LA, Shen ZJ, Sedgwick JB, Szakaly RJ, Sorkness RL, Malter JS
    J. Allergy Clin. Immunol., 2007;120(5):1082-8.
    Species: Rat
    Sample Types: BALF
  34. D-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma.
    Authors: Lee JS, Lee CM, Jeong YI, Jung ID, Kim BH, Seong EY, Kim JI, Choi IW, Chung HY, Park YM
    FEBS Lett., 2007;581(1):57-64.
    Species: Mouse
    Sample Types: BALF
  35. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
    Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24.
    Species: Mouse
    Sample Types: Tissue Homogenates
  36. Liposomal retinoic acids modulate asthma manifestations in mice.
    Authors: Maret M, Ruffie C, Periquet B, Campo AM, Menevret M, Phelep A, Dziewiszek K, Druilhe A, Pretolani M
    J. Nutr., 2007;137(12):2730-6.
    Species: Mouse
    Sample Types: BALF
  37. Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung.
    Authors: Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z
    J. Allergy Clin. Immunol., 2007;119(1):123-31.
    Species: Mouse
    Sample Types: BALF
  38. IL-21 administration into the nostril alleviates murine allergic rhinitis.
    Authors: Hiromura Y, Kishida T, Nakano H, Hama T, Imanishi J, Hisa Y, Mazda O
    J. Immunol., 2007;179(10):7157-65.
    Species: Mouse
    Sample Types: Serum
  39. The JAK-3 inhibitor CP-690550 is a potent anti-inflammatory agent in a murine model of pulmonary eosinophilia.
    Authors: Kudlacz E, Conklyn M, Andresen C, Whitney-Pickett C, Changelian P
    Eur. J. Pharmacol., 2007;582(1):154-61.
    Species: Mouse
    Sample Types: BALF
  40. Airway epithelial STAT3 is required for allergic inflammation in a murine model of asthma.
    Authors: Simeone-Penney MC, Severgnini M, Tu P, Homer RJ, Mariani TJ, Cohn L, Simon AR
    J. Immunol., 2007;178(10):6191-9.
    Species: Mouse
    Sample Types: BALF
  41. Persistent effects induced by IL-13 in the lung.
    Authors: Fulkerson PC, Fischetti CA, Hassman LM, Nikolaidis NM, Rothenberg ME
    Am. J. Respir. Cell Mol. Biol., 2006;35(3):337-46.
    Species: Mouse
    Sample Types: BALF
  42. Formulation with CpG oligodeoxynucleotides prevents induction of pulmonary immunopathology following priming with formalin-inactivated or commercial killed bovine respiratory syncytial virus vaccine.
    Authors: Oumouna M, Mapletoft JW, Karvonen BC, Babiuk LA
    J. Virol., 2005;79(4):2024-32.
    Species: Mouse
    Sample Types: BALF
  43. Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
    Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
    Life Sci., 2005;78(9):987-94.
    Species: Mouse
    Sample Types: BALF
  44. Vbeta14(+) T cells mediate the vaccine-enhanced disease induced by immunization with respiratory syncytial virus (RSV) G glycoprotein but not with formalin-inactivated RSV.
    Authors: Johnson TR, Varga SM, Braciale TJ, Graham BS
    J. Virol., 2004;78(16):8753-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
  45. Kinetics of change in the eotaxin concentration in serum and cerebrospinal fluid of mice infected with Angiostrongylus cantonensis.
    Authors: Chang EE, Chung LY, Yen CM
    Parasitol. Res., 2003;92(2):137-41.
    Species: Mouse
    Sample Types: CSF
  46. The effects of intranasal budesonide on allergen-induced production of interleukin-5 and eotaxin, airways, blood, and bone marrow eosinophilia, and eosinophil progenitor expansion in sensitized mice.
    Authors: O&amp;amp;apos;Byrne PM
    Am. J. Respir. Crit. Care Med., 2002;166(2):146-53.
    Species: Mouse
    Sample Types: Bone Marrow Fluid
  47. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
    Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
    Eur. J. Immunol., 2001;31(7):2104-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  48. Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
    Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
    J. Immunol., 2001;166(2):1233-40.
    Species: Mouse
    Sample Types: Tissue Homogenates
  49. Regulatory effects of eotaxin on acute lung inflammatory injury.
    Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
    J. Immunol., 2001;166(8):5208-18.
    Species: Mouse
    Sample Types: BALF

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Mouse CCL11/Eotaxin Quantikine ELISA Kit
By Anonymous on 08/25/2016
Application: Sample Tested: Esophagus