Mouse CCL11/Eotaxin Quantikine ELISA Kit

(47 citations)
(1 Review)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Citrate Plasma (13 uL)
  • Sensitivity
    3 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant mouse Eotaxin.  This assay cross-reacts 0.062% with Human Eotaxin and recognizes Rat Eotaxin.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Eotaxin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Eotaxin in cell culture supernates, serum, and plasma. It contains recombinant mouse Eotaxin and antibodies raised against the recombinant protein. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse Eotaxin showed dose curves that were parallel to the standard curves obtained using the E. coli-expressed kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Eotaxin.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation3.27.2303.811.728.7


The recovery of mouse Eotaxin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 96 82-103
Citrate Plasma (n=5) 97 81-114
EDTA Plasma (n=5) 100 83-118
Serum (n=5) 98 82-111
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse Eotaxin in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CCL11/Eotaxin Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL11/Eotaxin
CCL11/Eotaxin is a chemokine that interacts with CCR3 to induce eosinophil, basophil, dendritic cell, and Th2 cell chemoattraction during inflammatory and allergic reactions. Its production is upregulated in airway epithelium, alveolar macrophages, eosinophils, smooth muscle cells, chondrocytes, and fibroblasts following allergen or inflammatory challenge. CCL11 is also involved in the growth of myeloid cell progenitors and the differentiation of mast cells.
    • Entrez Gene IDs
      6356 (Human); 20292 (Mouse);
    • Alternate Names
      C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin);
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    47 Citations: Showing 1 - 10
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    Sample Type
    1. Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart
      Eur J Immunol, 2016;0(0):.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    2. Increased mitochondrial arginine metabolism supports bioenergetics in asthma
      J Clin Invest, 2016;0(0):.
      Species: Mouse
      Sample Type: BALF
    3. G-protein-coupled estrogen receptor agonist suppresses airway inflammation in a mouse model of asthma through IL-10.
      Authors: Itoga M, Konno Y, Moritoki Y, Saito Y, Ito W, Tamaki M, Kobayashi Y, Kayaba H, Kikuchi Y, Chihara J, Takeda M, Ueki S, Hirokawa M
      PLoS ONE, 2015;10(3):e0123210.
      Species: Mouse
      Sample Type: BALF
    4. Rapid transport of CCL11 across the blood-brain barrier: regional variation and importance of blood cells.
      Authors: Erickson, Michelle, Morofuji, Yoichi, Owen, Joshua B, Banks, William
      J Pharmacol Exp Ther, 2014;349(3):497-507.
      Species: Mouse
      Sample Type: Tissue Homogenates
    5. Genetic vaccine for respiratory syncytial virus provides protection without disease potentiation.
      Authors: Johnson T, Rangel D, Graham B, Brough D, Gall J
      Mol Ther, 2014;22(1):196-205.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
      Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
      Am. J. Pathol., 2012;179(1):302-14.
      Species: Mouse
      Sample Type: Tissue Homogenates
    7. Spontaneous eosinophilic nasal inflammation in a genetically-mutant mouse: comparative study with an allergic inflammation model.
      Authors: Cho SH, Oh SY, Zhu Z, Lee J, Lane AP
      PLoS ONE, 2012;7(4):e35114.
      Species: Mouse
      Sample Type: Nasal Lavage
    8. Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
      Authors: Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN
      Clin. Exp. Allergy, 2011;41(10):1447-55.
      Species: Mouse
      Sample Type: BALF
    9. IL-33-activated dendritic cells are critical for allergic airway inflammation.
      Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
      Eur. J. Immunol., 2011;41(6):1675-86.
      Species: Mouse
      Sample Type: BALF
    10. Endogenous IL-33 enhances Th2 cytokine production and T-cell responses during allergic airway inflammation.
      Authors: Louten J, Rankin AL, Li Y
      Int. Immunol., 2011;23(5):307-15.
      Species: Mouse
      Sample Type: BALF
    11. Impact of adiponectin deficiency on pulmonary responses to acute ozone exposure in mice.
      Authors: Zhu M, Hug C, Kasahara DI
      Am. J. Respir. Cell Mol. Biol., 2010;43(4):487-97.
      Species: Mouse
      Sample Type: BALF
    12. House dust mite induces direct airway inflammation in vivo: implications for future disease therapy?
      Authors: De Alba J, Raemdonck K, Dekkak A, Collins M, Wong S, Nials AT, Knowles RG, Belvisi MG, Birrell MA
      Eur. Respir. J., 2010;35(6):1377-87.
      Species: Rat
      Sample Type: BALF
    13. Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production.
      Authors: Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD
      Inflamm. Res., 2009;58(12):845-54.
      Species: Mouse
      Sample Type: BALF
    14. CD11b+ myeloid cells are the key mediators of Th2 cell homing into the airway in allergic inflammation.
      Authors: Medoff BD, Seung E, Hong S, Thomas SY, Sandall BP, Duffield JS, Kuperman DA, Erle DJ, Luster AD
      J. Immunol., 2009;182(1):623-35.
      Species: Mouse
      Sample Type: BALF
    15. A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice.
      Authors: Shinagawa K, Ploplis VA, Castellino FJ
      Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L763-70.
      Species: Mouse
      Sample Type: BALF
    16. The adaptor protein CIKS/Act1 is essential for IL-25-mediated allergic airway inflammation.
      Authors: Claudio E, Sonder SU, Saret S, Carvalho G, Ramalingam TR, Wynn TA, Chariot A, Garcia-Perganeda A, Leonardi A, Paun A, Chen A, Ren NY, Wang H, Siebenlist U
      J. Immunol., 2009;182(3):1617-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    17. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
      Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
      Vaccine, 2009;27(23):3045-52.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    18. Inhaled multiwalled carbon nanotubes potentiate airway fibrosis in murine allergic asthma.
      Authors: Ryman-Rasmussen JP, Tewksbury EW, Moss OR, Cesta MF, Wong BA, Bonner JC
      Am. J. Respir. Cell Mol. Biol., 2009;40(3):349-58.
      Species: Mouse
      Sample Type: BALF
    19. Ablation of type I hypersensitivity in experimental allergic conjunctivitis by eotaxin-1/CCR3 blockade.
      Authors: Miyazaki D, Nakamura T, Ohbayashi M, Kuo CH, Komatsu N, Yakura K, Tominaga T, Inoue Y, Higashi H, Murata M, Takeda S, Fukushima A, Liu FT, Rothenberg ME, Ono SJ
      Int. Immunol., 2009;21(2):187-201.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    20. Adenovirus IL-13-induced airway disease in mice: a corticosteroid-resistant model of severe asthma.
      Authors: Therien AG, Bernier V, Weicker S, Tawa P, Falgueyret JP, Mathieu MC, Honsberger J, Pomerleau V, Robichaud A, Stocco R, Dufresne L, Houshyar H, Lafleur J, Ramachandran C, O'Neill GP, Slipetz D, Tan CM
      Am. J. Respir. Cell Mol. Biol., 2008;39(1):26-35.
      Species: Mouse
      Sample Type: BALF
    21. Tyrosine phosphatase SHP-1 in oxidative stress and development of allergic airway inflammation.
      Authors: Cho YS, Oh SY, Zhu Z
      Am. J. Respir. Cell Mol. Biol., 2008;39(4):412-9.
      Species: Mouse
      Sample Type: BALF
    22. Distinct roles for IL-13 and IL-4 via IL-13 receptor alpha1 and the type II IL-4 receptor in asthma pathogenesis.
      Authors: Munitz A, Brandt EB, Mingler M, Finkelman FD, Rothenberg ME
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7240-5.
      Species: Mouse
      Sample Type: BALF
    23. Treatment with anti-CC chemokine receptor 3 monoclonal antibody or dexamethasone inhibits the migration and differentiation of bone marrow CD34 progenitor cells in an allergic mouse model.
      Authors: Ben S, Li X, Xu F, Xu W, Li W, Wu Z, Huang H, Shi H, Shen H
      Allergy, 2008;63(9):1164-76.
      Species: Mouse
      Sample Type: Serum
    24. An eosinophil immune response characterizes the inflammatory skin disease observed in Tie-2 transgenic mice.
      Authors: Voskas D, Babichev Y, Ling LS, Alami J, Shaked Y, Kerbel RS, Ciruna B, Dumont DJ
      J. Leukoc. Biol., 2008;84(1):59-67.
      Species: Mouse
      Sample Type: Serum
    25. Eosinophil-nerve interactions and neuronal plasticity in rat gut associated lymphoid tissue (GALT) in response to enteric parasitism.
      Authors: O'Brien LM, Fitzpatrick E, Baird AW, Campion DP
      J. Neuroimmunol., 2008;197(1):1-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    26. Pulmonary eosinophilia requires interleukin-5, eotaxin-1, and CD4+ T cells in mice immunized with respiratory syncytial virus G glycoprotein.
      Authors: Johnson TR, Rothenberg ME, Graham BS
      J. Leukoc. Biol., 2008;84(3):748-59.
      Species: Mouse
      Sample Type: Tissue Homogenates
    27. Airway house dust extract exposures modify allergen-induced airway hypersensitivity responses by TLR4-dependent and independent pathways.
      Authors: Lam D, Ng N, Lee S, Batzer G, Horner AA
      J. Immunol., 2008;181(4):2925-32.
      Species: Mouse
      Sample Type: BALF
    28. IL-13 receptor alpha2 selectively inhibits IL-13-induced responses in the murine lung.
      Authors: Zheng T, Liu W, Oh SY, Zhu Z, Hu B, Homer RJ, Cohn L, Grusby MJ, Elias JA
      J. Immunol., 2008;180(1):522-9.
      Species: Mouse
      Sample Type: BALF
    29. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
      Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
      J. Exp. Med., 2008;205(9):2075-84.
      Species: Mouse
      Sample Type: Serum
    30. Airway epithelial STAT3 is required for allergic inflammation in a murine model of asthma.
      Authors: Simeone-Penney MC, Severgnini M, Tu P, Homer RJ, Mariani TJ, Cohn L, Simon AR
      J. Immunol., 2007;178(10):6191-9.
      Species: Mouse
      Sample Type: BALF
    31. Critical role of the Fc receptor gamma-chain on APCs in the development of allergen-induced airway hyperresponsiveness and inflammation.
      Authors: Kitamura K, Takeda K, Koya T, Miyahara N, Kodama T, Dakhama A, Takai T, Hirano A, Tanimoto M, Harada M, Gelfand EW
      J. Immunol., 2007;178(1):480-8.
      Species: Mouse
      Sample Type: BALF
    32. The JAK-3 inhibitor CP-690550 is a potent anti-inflammatory agent in a murine model of pulmonary eosinophilia.
      Authors: Kudlacz E, Conklyn M, Andresen C, Whitney-Pickett C, Changelian P
      Eur. J. Pharmacol., 2007;582(1):154-61.
      Species: Mouse
      Sample Type: BALF
    33. A critical role for Pin1 in allergic pulmonary eosinophilia in rats.
      Authors: Esnault S, Rosenthal LA, Shen ZJ, Sedgwick JB, Szakaly RJ, Sorkness RL, Malter JS
      J. Allergy Clin. Immunol., 2007;120(5):1082-8.
      Species: Rat
      Sample Type: BALF
    34. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
      Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
      Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24.
      Species: Mouse
      Sample Type: Tissue Homogenates
    35. Liposomal retinoic acids modulate asthma manifestations in mice.
      Authors: Maret M, Ruffie C, Periquet B, Campo AM, Menevret M, Phelep A, Dziewiszek K, Druilhe A, Pretolani M
      J. Nutr., 2007;137(12):2730-6.
      Species: Mouse
      Sample Type: BALF
    36. Src homology 2 domain-containing inositol 5-phosphatase 1 deficiency leads to a spontaneous allergic inflammation in the murine lung.
      Authors: Oh SY, Zheng T, Bailey ML, Barber DL, Schroeder JT, Kim YK, Zhu Z
      J. Allergy Clin. Immunol., 2007;119(1):123-31.
      Species: Mouse
      Sample Type: BALF
    37. D-pinitol regulates Th1/Th2 balance via suppressing Th2 immune response in ovalbumin-induced asthma.
      Authors: Lee JS, Lee CM, Jeong YI, Jung ID, Kim BH, Seong EY, Kim JI, Choi IW, Chung HY, Park YM
      FEBS Lett., 2007;581(1):57-64.
      Species: Mouse
      Sample Type: BALF
    38. IL-21 administration into the nostril alleviates murine allergic rhinitis.
      Authors: Hiromura Y, Kishida T, Nakano H, Hama T, Imanishi J, Hisa Y, Mazda O
      J. Immunol., 2007;179(10):7157-65.
      Species: Mouse
      Sample Type: Serum
    39. Persistent effects induced by IL-13 in the lung.
      Authors: Fulkerson PC, Fischetti CA, Hassman LM, Nikolaidis NM, Rothenberg ME
      Am. J. Respir. Cell Mol. Biol., 2006;35(3):337-46.
      Species: Mouse
      Sample Type: BALF
    40. Formulation with CpG oligodeoxynucleotides prevents induction of pulmonary immunopathology following priming with formalin-inactivated or commercial killed bovine respiratory syncytial virus vaccine.
      Authors: Oumouna M, Mapletoft JW, Karvonen BC, Babiuk LA
      J. Virol., 2005;79(4):2024-32.
      Species: Mouse
      Sample Type: BALF
    41. Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
      Authors: Shibamori M, Ogino K, Kambayashi Y, Ishiyama H
      Life Sci., 2005;78(9):987-94.
      Species: Mouse
      Sample Type: BALF
    42. Vbeta14(+) T cells mediate the vaccine-enhanced disease induced by immunization with respiratory syncytial virus (RSV) G glycoprotein but not with formalin-inactivated RSV.
      Authors: Johnson TR, Varga SM, Braciale TJ, Graham BS
      J. Virol., 2004;78(16):8753-60.
      Species: Mouse
      Sample Type: Tissue Homogenates
    43. Kinetics of change in the eotaxin concentration in serum and cerebrospinal fluid of mice infected with Angiostrongylus cantonensis.
      Authors: Chang EE, Chung LY, Yen CM
      Parasitol. Res., 2003;92(2):137-41.
      Species: Mouse
      Sample Type: CSF
    44. The effects of intranasal budesonide on allergen-induced production of interleukin-5 and eotaxin, airways, blood, and bone marrow eosinophilia, and eosinophil progenitor expansion in sensitized mice.
      Authors: O&amp;amp;apos;Byrne PM
      Am. J. Respir. Crit. Care Med., 2002;166(2):146-53.
      Species: Mouse
      Sample Type: Bone Marrow Fluid
    45. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
      Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
      Eur. J. Immunol., 2001;31(7):2104-15.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    46. Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
      Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
      J. Immunol., 2001;166(2):1233-40.
      Species: Mouse
      Sample Type: Tissue Homogenates
    47. Regulatory effects of eotaxin on acute lung inflammatory injury.
      Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
      J. Immunol., 2001;166(8):5208-18.
      Species: Mouse
      Sample Type: BALF
    Expand to show all 47 Citations
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