Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL2/JE/MCP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL2/JE/MCP-1 (R&D Systems, Catalog # 479-JE)
Gln24-Arg96
Accession # Q5SVU3

Specificity

Detects mouse CCL2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross‑reactivity with rhMCP‑1, rhMCP‑2, rhMCP‑3, rmMARC, rhMCP‑4, rmMCP‑5, rmMDC, rmMIP‑1a, rmMIP‑1b, rmMIP‑1g, rmMIP‑2, rmMIP‑3b, rmRANTES, rmTARC and rmTECK is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE)

Mouse CCL2/JE/MCP-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse CCL2/JE/MCP‑1 Antibody (Catalog # AF-479-NA)
  • Standard: Recombinant Mouse CCL2/JE/MCP-1 Protein (Catalog # 479-JE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using BAF479 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

References

  1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738.
  2. Gu, L. et al. (1999) Chem. Immunol. 72:7.
  3. Luini, W. et al. (1994) Cytokine 6:28.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

Q5SVU3

Additional CCL2/JE/MCP-1 Products

Product Documents for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody

For research use only

Citations for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody

Customer Reviews for Mouse CCL2/JE/MCP‑1 Biotinylated Antibody (1)

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  • Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: See PMID: 23410977
    Species: Mouse
    Verified Customer | Posted 01/15/2015

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