Mouse CCL2/JE/MCP-1 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY479
DY479-05
Ancillary Products Available
Mouse CCL2 / JE / MCP-1 ELISA Standard Curve
1 Image
Product Details
Procedure
Citations (91)
FAQs
Supplemental Products
Reviews (7)

Mouse CCL2/JE/MCP-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CCL2/JE. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Mouse CCL2 / JE / MCP-1 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL2/JE/MCP-1

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

Entrez Gene IDs:
6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Alternate Names:
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse CCL2/JE/MCP-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

91 Citations: Showing 1 - 10
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  1. The role of Staphylococcus aureus lipoproteins in hematogenous septic arthritis
    Authors: M Mohammad, Z Hu, A Ali, PK Kopparapu, M Na, A Jarneborn, MDN Stroparo, MT Nguyen, A Karlsson, F Götz, R Pullerits, T Jin
    Sci Rep, 2020;10(1):7936.
    Species: Mouse
    Sample Types: Serum
  2. The Role of MKP-5 in Adipocyte-Macrophage Interactions during Obesity
    Authors: Y Lu, J Ma, J Zhao, Z Song, C Zhou, X Liu, W Teng, W Wang, Q Zhang, W Yan, P Jiao
    Obes Facts, 2020;0(0):1-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. EphB2-dependent signaling promotes neuronal excitotoxicity and inflammation in the acute phase of ischemic stroke
    Authors: AS Ernst, LI Böhler, AM Hagenston, A Hoffmann, S Heiland, C Sticht, M Bendszus, M Hecker, H Bading, HH Marti, T Korff, R Kunze
    Acta Neuropathol Commun, 2019;7(1):15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Rupintrivir reduces RV-induced TH-2 cytokine IL-4 in precision-cut lung slices (PCLS) of HDM-sensitized mice ex vivo
    Authors: O Danov, L Lasswitz, H Obernolte, C Hesse, A Braun, S Wronski, K Sewald
    Respir. Res., 2019;20(1):228.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Elevated circulating TGF?1 during acute liver failure activates TGF?R2 on cortical neurons and exacerbates neuroinflammation and hepatic encephalopathy in mice
    Authors: M McMillin, S Grant, G Frampton, AD Petrescu, E Williams, B Jefferson, A Thomas, A Brahmarout, S DeMorrow
    J Neuroinflammation, 2019;16(1):69.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. NLRX1 does not play a role in diabetes nor the development of diabetic nephropathy induced by multiple low doses of streptozotocin
    Authors: AML Scantleber, M Uil, LM Butter, R Poelman, N Claessen, SE Girardin, S Florquin, JJTH Roelofs, JC Leemans
    PLoS ONE, 2019;14(3):e0214437.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Adipocyte-secreted exosomal microRNA-34a inhibits M2 macrophage polarization to promote obesity-induced adipose inflammation
    Authors: Y Pan, X Hui, RLC Hoo, D Ye, CYC Chan, T Feng, Y Wang, KSL Lam, A Xu
    J. Clin. Invest., 2019;129(2):834-849.
    Species: Mouse
    Sample Types: Serum
  8. In-depth characterization of congenital Zika syndrome in immunocompetent mice: Antibody-dependent enhancement and an antiviral peptide therapy
    Authors: VN Camargos, G Foureaux, DC Medeiros, VT da Silveir, CM Queiroz-Ju, ALB Matosinhos, AFA Figueiredo, CDF Sousa, TP Moreira, VF Queiroz, ACF Dias, KTO Santana, I Passos, ALCV Real, LC Silva, FAG Mourão, NT Wnuk, MAP Oliveira, S Macari, T Silva, GP Garlet, JA Jackman, FM Soriani, MFD Moraes, EMAM Mendes, FM Ribeiro, GMJ Costa, AL Teixeira, NJ Cho, ACP Oliveira, MM Teixeira, VV Costa, DG Souza
    EBioMedicine, 2019;0(0):.
    Species: Mouse
    Sample Types: Plasma
  9. Acute transient cognitive dysfunction and acute brain injury induced by systemic inflammation occur by dissociable IL-1-dependent mechanisms
    Authors: DT Skelly, ÉW Griffin, CL Murray, S Harney, C O'Boyle, E Hennessy, MA Dansereau, A Nazmi, L Tortorelli, JN Rawlins, DM Bannerman, C Cunningham
    Mol. Psychiatry, 2019;24(10):1533-1548.
    Species: Mouse
    Sample Types: Plasma
  10. Impact of immunization against OxLDL on the pulmonary response to cigarette smoke exposure in mice
    Authors: M Talbot, M Hamel-Auge, MJ Beaulieu, M Gazzola, A Lechasseur, S Aubin, MÈ Paré, D Marsolais, Y Bossé, MC Morissette
    Respir. Res., 2018;19(1):131.
    Species: Mouse
    Sample Types: BALF
  11. Identification of periplakin as a major regulator of lung injury and repair in mice
    Authors: V Besnard, R Dagher, T Madjer, A Joannes, M Jaillet, M Kolb, P Bonniaud, LA Murray, MA Sleeman, B Crestani
    JCI Insight, 2018;3(5):.
    Species: Mouse
    Sample Types: BALF
  12. Inhalation of the prodrug PI3K inhibitor CL27c improves lung function in asthma and fibrosis
    Authors: CC Campa, RL Silva, JP Margaria, T Pirali, MS Mattos, LR Kraemer, DC Reis, G Grosa, F Copperi, EM Dalmarco, RCP Lima-Júnio, S Aprile, V Sala, F Dal Bello, DS Prado, JC Alves-Filh, C Medana, GD Cassali, GC Tron, MM Teixeira, E Ciraolo, RC Russo, E Hirsch
    Nat Commun, 2018;9(1):5232.
    Species: Mouse
    Sample Types:
  13. CCR2 mediates increased susceptibility to post-H1N1 bacterial pneumonia by limiting dendritic cell induction of IL-17
    Authors: SJ Gurczynski, N Nathani, HI Warheit-Ni, EM Hult, A Podsiad, J Deng, RL Zemans, U Bhan, BB Moore
    Mucosal Immunol, 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma
    Authors: GM Wiedemann, C Aithal, A Kraechan, C Heise, BL Cadilha, J Zhang, P Duewell, R Ballotti, S Endres, C Bertolotto, S Kobold
    Transl Oncol, 2018;12(2):350-360.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Antibiotics with Interleukin-15 inhibition reduces joint inflammation and bone erosions but not cartilage destruction ininduced arthritis
    Authors: B Bergmann, P Jirholt, P Henning, C Lindholm, C Ohlsson, IB McInnes, UH Lerner, I Gjertsson
    Infect. Immun., 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
  16. Factor XIIIA-expressing inflammatory monocytes promote lung squamous cancer through fibrin cross-linking
    Authors: A Porrello, PL Leslie, EB Harrison, BK Gorentla, S Kattula, SK Ghosh, SH Azam, A Holtzhause, YL Chao, MC Hayward, TA Waugh, S Bae, V Godfrey, SH Randell, C Oderup, L Makowski, J Weiss, MD Wilkerson, DN Hayes, HS Earp, AS Baldwin, AS Wolberg, CV Pecot
    Nat Commun, 2018;9(1):1988.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. FOXO3-dependent apoptosis limits alcohol-induced liver inflammation by promoting infiltrating macrophage differentiation
    Authors: Z Li, J Zhao, S Zhang, SA Weinman
    Cell Death Discov, 2018;4(0):16.
    Species: Mouse
    Sample Types: Serum
  18. Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy
    Authors: KJ Katschke, H Xi, C Cox, T Truong, Y Malato, WP Lee, B McKenzie, R Arceo, J Tao, L Rangell, M Reichelt, L Diehl, J Elstrott, RM Weimer, MVL Campagne
    Sci Rep, 2018;8(1):7348.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. p53 in Bronchial Club Cells Facilitates Chronic Lung Inflammation by Promoting Senescence
    Authors: A Sagiv, A Bar-Shai, N Levi, M Hatzav, L Zada, Y Ovadya, L Roitman, G Manella, O Regev, J Majewska, E Vadai, R Eilam, SW Feigelson, M Tsoory, M Tauc, R Alon, V Krizhanovs
    Cell Rep, 2018;22(13):3468-3479.
  20. Parkin deficiency modulates NLRP3 inflammasome activation by attenuating an A20-dependent negative feedback loop
    Authors: F Mouton-Lig, T Rosazza, J Sepulveda-, A Ieang, SM Hassoun, E Claire, G Mangone, A Brice, PP Michel, JC Corvol, O Corti
    Glia, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Genetic ablation of histone deacetylase 2 leads to lung cellular senescence and lymphoid follicle formation in COPD/emphysema
    Authors: IK Sundar, K Rashid, J Gerloff, J Rangel-Mor, D Li, I Rahman
    FASEB J., 2018;0(0):fj201701518R.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. The Long Pentraxin 3 Plays a Role in Bone Turnover and Repair
    Authors: D Gr?evi?, M Sironi, S Valentino, L Deban, H Cvija, A Inforzato, N Kova?i?, V Katavi?, T Kelava, I Kalajzi?, A Mantovani, B Bottazzi
    Front Immunol, 2018;9(0):417.
    Species: Mouse
    Sample Types: Serum
  23. Alternaria alternata challenge at the nasal mucosa results in eosinophilic inflammation and increased susceptibility to influenza virus infection
    Authors: M Ma, JL Redes, CM Percopo, KM Druey, HF Rosenberg
    Clin. Exp. Allergy, 2018;0(0):.
    Species: Mouse
    Sample Types: Nasal Lavage Fluid
  24. Both anti-TNF and CTLA4 Ig treatments attenuate the disease severity of staphylococcal dermatitis in mice
    Authors: M Na, W Wang, Y Fei, E Josefsson, A Ali, T Jin
    PLoS ONE, 2017;12(3):e0173492.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. Surface layer proteins from virulent Clostridium difficile ribotypes exhibit signatures of positive selection with consequences for innate immune response
    Authors: M Lynch, TA Walsh, I Marszalows, AE Webb, M MacAogain, TR Rogers, H Windle, D Kelleher, MJ O'Connell, CE Loscher
    BMC Evol. Biol, 2017;17(1):90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection
    Authors: EE Hornick, B Banoth, AM Miller, ZR Zacharias, N Jain, ME Wilson, KN Gibson-Cor, KL Legge, GA Bishop, FS Sutterwala, SL Cassel
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  27. NLRX1 dampens oxidative stress and apoptosis in tissue injury via control of mitochondrial activity
    Authors: G Stokman, L Kors, PJ Bakker, E Rampanelli, N Claessen, GJD Teske, L Butter, H van Andel, MA van den Be, PWB Larsen, MC Dessing, CJ Zuurbier, SE Girardin, S Florquin, JC Leemans
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  28. Contributions of CD8 T cells to the pathogenesis of mouse adenovirus type 1 respiratory infection
    Authors: CT Molloy, JS Andonian, HM Seltzer, MC Procario, ME Watson, JB Weinberg
    Virology, 2017;507(0):64-74.
    Species: Mouse
    Sample Types: BALF
  29. Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants
    Authors: LT Hsieh, MV Nastase, H Roedig, J Zeng-Brouw, C Poluzzi, S Schwalm, C Fork, C Tredup, RP Brandes, M Wygrecka, A Huwiler, J Pfeilschif, L Schaefer
    Int J Mol Sci, 2017;18(3):.
    Species: Mouse
    Sample Types: Plasma
  30. The GLP-1 analogue lixisenatide decreases atherosclerosis in insulin-resistant mice by modulating macrophage phenotype
    Authors: Á Vinué, J Navarro, A Herrero-Ce, M García-Cub, I Andrés-Bla, S Martínez-H, JT Real, JF Ascaso, H González-N
    Diabetologia, 2017;0(0):.
    Species: Mouse
    Sample Types: Plasma
  31. The TRAIL-Induced Cancer Secretome Promotes a Tumor-Supportive Immune Microenvironment via CCR2
    Authors: T Hartwig, A Montinaro, S von Karste, A Sevko, S Surinova, A Chakravart, L Taraborrel, P Draber, E Lafont, F Arce Varga, MA El-Bahrawy, SA Quezada, H Walczak
    Mol. Cell, 2017;65(4):730-742.e5.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. Impacts of allergic airway inflammation on lung pathology in a mouse model of influenza A virus infection
    Authors: A Kawaguchi, T Suzuki, Y Ohara, K Takahashi, Y Sato, A Ainai, N Nagata, M Tashiro, H Hasegawa
    PLoS ONE, 2017;12(2):e0173008.
    Species: Mouse
    Sample Types: BALF
  33. Antibodies against nonstructural protein 1 protect mice from dengue virus-induced mast cell activation
    Authors: YT Chu, SW Wan, YC Chang, CK Lee, BA Wu-Hsieh, R Anderson, YS Lin
    Lab. Invest, 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  34. Fetuin-A aggravates lipotoxicity in podocytes via interleukin-1 signaling
    Authors: JM Orellana, K Kampe, F Schulze, J Sieber, AW Jehle
    Physiol Rep, 2017;5(10):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  35. Intramembrane attenuation of the TLR4-TLR6 dimer impairs receptor assembly and reduces microglia mediated neurodegeneration
    Authors: L Shmuel-Gal, Y Klug, Z Porat, M Charni, B Zarmi, Y Shai
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  36. NADPH oxidase 4 regulates vascular inflammation in aging and atherosclerosis
    Authors: Andrey Lozhkin
    J. Mol. Cell. Cardiol, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  37. APPL1 prevents pancreatic beta cell death and inflammation by dampening NF?B activation in a mouse model of type 1 diabetes
    Authors: Xue Jiang
    Diabetologia, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  38. PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen
    Authors: Bryan Simons
    J. Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: BALF
  39. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2015;515(7525):130-3.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. IL-1 receptor type 2 suppresses collagen-induced arthritis by inhibiting IL-1 signal on macrophages.
    Authors: Shimizu K, Nakajima A, Sudo K, Liu Y, Mizoroki A, Ikarashi T, Horai R, Kakuta S, Watanabe T, Iwakura Y
    J Immunol, 2015;194(7):3156-68.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  41. Influenza A virus-dependent remodeling of pulmonary clock function in a mouse model of COPD.
    Authors: Sundar, Isaac K, Ahmad, Tanveer, Yao, Hongwei, Hwang, Jae-woon, Gerloff, Janice, Lawrence, B Paige, Sellix, Michael, Rahman, Irfan
    Sci Rep, 2015;4(0):9927.
    Species: Mouse
    Sample Types: BALF
  42. Alveolar macrophage-derived type I interferons orchestrate innate immunity to RSV through recruitment of antiviral monocytes.
    Authors: Goritzka M, Makris S, Kausar F, Durant L, Pereira C, Kumagai Y, Culley F, Mack M, Akira S, Johansson C
    J Exp Med, 2015;212(5):699-714.
    Species: Mouse
    Sample Types: Tissue Homogenates
  43. Autoprocessing of neutrophil elastase near its active site reduces the efficiency of natural and synthetic elastase inhibitors.
    Authors: Dau T, Sarker R, Yildirim A, Eickelberg O, Jenne D
    Nat Commun, 2015;6(0):6722.
    Species: Mouse
    Sample Types: BALF
  44. Mapping the innate signaling cascade essential for cytokine storm during influenza virus infection.
    Authors: Teijaro J, Walsh K, Rice S, Rosen H, Oldstone M
    Proc Natl Acad Sci U S A, 2014;111(10):3799-804.
    Species: Mouse
    Sample Types: BALF
  45. Hypothalamic gene transfer of BDNF inhibits breast cancer progression and metastasis in middle age obese mice.
    Authors: Liu, Xianglan, McMurphy, Travis, Xiao, Run, Slater, Andrew, Huang, Wei, Cao, Lei
    Mol Ther, 2014;22(7):1275-84.
    Species: Mouse
    Sample Types: Serum
  46. Munc18-2 and syntaxin 3 control distinct essential steps in mast cell degranulation.
    Authors: Brochetta C, Suzuki R, Vita F, Soranzo M, Claver J, Madjene L, Attout T, Vitte J, Varin-Blank N, Zabucchi G, Rivera J, Blank U
    J Immunol, 2014;192(1):41-51.
    Species: Rat
    Sample Types: Cell Culture Supernates
  47. No Impairment in host defense against Streptococcus pneumoniae in obese CPEfat/fat mice.
    Authors: Mancuso P, O Brien E, Prano J, Goel D, Aronoff D
    PLoS ONE, 2014;9(9):e106420.
    Species: Mouse
    Sample Types: Serum
  48. The role of Nox2-derived ROS in the development of cognitive impairment after sepsis.
    Authors: Hernandes M, D'Avila J, Trevelin S, Reis P, Kinjo E, Lopes L, Castro-Faria-Neto H, Cunha F, Britto L, Bozza F
    J Neuroinflammation, 2014;11(0):36.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  49. Modulation of the mevalonate pathway by akt regulates macrophage survival and development of pulmonary fibrosis.
    Authors: Larson-Casey J, Murthy S, Ryan A, Carter A
    J Biol Chem, 2014;289(52):36204-19.
    Species: Mouse
    Sample Types: BALF
  50. Bone marrow-specific knock-in of a non-activatable Ikkalpha kinase mutant influences haematopoiesis but not atherosclerosis in Apoe-deficient mice.
    Authors: Tilstam P, Gijbels M, Habbeddine M, Cudejko C, Asare Y, Theelen W, Zhou B, Doring Y, Drechsler M, Pawig L, Simsekyilmaz S, Koenen R, de Winther M, Lawrence T, Bernhagen J, Zernecke A, Weber C, Noels H
    PLoS ONE, 2014;9(2):e87452.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. MicroRNA-155 is essential for T(H)2-mediated allergen-induced eosinophilic inflammation in the lung.
    Authors: Malmhall C, Alawieh S, Lu Y, Sjostrand M, Bossios A, Eldh M, Radinger M
    J Allergy Clin Immunol, 2014;133(5):1429-38, 1438.
    Species: Mouse
    Sample Types: Tissue Homogenates
  52. Animal model of respiratory syncytial virus: CD8+ T cells cause a cytokine storm that is chemically tractable by sphingosine-1-phosphate 1 receptor agonist therapy.
    Authors: Walsh K, Teijaro J, Brock L, Fremgen D, Collins P, Rosen H, Oldstone M
    J Virol, 2014;88(11):6281-93.
    Species: Mouse
    Sample Types: BALF
  53. Circadian clock function is disrupted by environmental tobacco/cigarette smoke, leading to lung inflammation and injury via a SIRT1-BMAL1 pathway.
    Authors: Hwang J, Sundar I, Yao H, Sellix M, Rahman I
    FASEB J, 2014;28(1):176-94.
    Species: Mouse
    Sample Types: Tissue Homogenates
  54. A2B adenosine receptors prevent insulin resistance by inhibiting adipose tissue inflammation via maintaining alternative macrophage activation.
    Authors: Csoka B, Koscso B, Toro G, Kokai E, Virag L, Nemeth Z, Pacher P, Bai P, Hasko G
    Diabetes, 2014;63(3):850-66.
    Species: Mouse
    Sample Types: Plasma
  55. A TNF-alpha-CCL20-CCR6 axis regulates Nod1-induced B cell responses.
    Authors: Paradis M, Mindt B, Duerr C, Rojas O, Ng D, Boulianne B, McCarthy D, Yu M, Summers deLuca L, Ward L, Waldron J, Philpott D, Gommerman J, Fritz J
    J Immunol, 2014;192(6):2787-99.
    Species: Mouse
    Sample Types: Serum
  56. Defining the range of pathogens susceptible to Ifitm3 restriction using a knockout mouse model.
    Authors: Everitt, Aaron R, Clare, Simon, McDonald, Jacqueli, Kane, Leanne, Harcourt, Katherin, Ahras, Malika, Lall, Amar, Hale, Christin, Rodgers, Angela, Young, Douglas, Haque, Ashraful, Billker, Oliver, Tregoning, John S, Dougan, Gordon, Kellam, Paul
    PLoS ONE, 2013;8(11):e80723.
    Species: Mouse
    Sample Types: BALF
  57. Syntaxin binding protein 1 is not required for allergic inflammation via IgE-mediated mast cell activation.
    Authors: Wu Z, MacNeil A, Berman J, Lin T
    PLoS ONE, 2013;8(3):e58560.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  58. Effect of secretin on preadipocyte, differentiating and mature adipocyte functions.
    Authors: Miegueu P, Cianflone K, Richard D, St-Pierre D
    Int J Obes (Lond), 2013;37(3):366-74.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  59. The novel cytokine interleukin-33 activates acinar cell proinflammatory pathways and induces acute pancreatic inflammation in mice.
    Authors: Kempuraj D, Twait E, Williard D, Yuan Z, Meyerholz D, Samuel I
    PLoS ONE, 2013;8(2):e56866.
    Species: Mouse
    Sample Types: Tissue Homogenates
  60. Deficiency of C5L2 increases macrophage infiltration and alters adipose tissue function in mice.
    Authors: Gauvreau D, Gupta A, Fisette A, Tom F, Cianflone K
    PLoS ONE, 2013;8(4):e60795.
    Species: Mouse
    Sample Types: Plasma
  61. A novel murine model of rhinoscleroma identifies Mikulicz cells, the disease signature, as IL-10 dependent derivatives of inflammatory monocytes.
    Authors: Fevre C, Almeida A, Taront S, Pedron T, Huerre M, Prevost M, Kieusseian A, Cumano A, Brisse S, Sansonetti P, Tournebize R
    EMBO Mol Med, 2013;5(4):516-30.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2012;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  63. Suppression of cytokine storm with a sphingosine analog provides protection against pathogenic influenza virus.
    Authors: Walsh KB, Teijaro JR, Wilker PR
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(29):12018-23.
    Species: Mouse
    Sample Types: BALF
  64. Phosphoinositide 3-kinase gamma plays a critical role in bleomycin-induced pulmonary inflammation and fibrosis in mice.
    Authors: Russo RC, Garcia CC, Barcelos LS, Rachid MA, Guabiraba R, Roffe E, Souza AL, Sousa LP, Mirolo M, Doni A, Cassali GD, Pinho V, Locati M, Teixeira MM
    J. Leukoc. Biol., 2011;89(2):269-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
  65. Lung-targeted overexpression of the NF-kappaB member RelB inhibits cigarette smoke-induced inflammation.
    Authors: McMillan DH, Baglole CJ, Thatcher TH, Maggirwar S, Sime PJ, Phipps RP
    Am. J. Pathol., 2011;179(1):125-33.
    Species: Mouse
    Sample Types: BALF
  66. The Therapeutic Potential of the Humoral Pattern Recognition Molecule PTX3 in Chronic Lung Infection Caused by Pseudomonas aeruginosa.
    Authors: Moalli F, Paroni M, Veliz Rodriguez T, Riva F, Polentarutti N, Bottazzi B, Valentino S, Mantero S, Nebuloni M, Mantovani A, Bragonzi A, Garlanda C
    J. Immunol., 2011;186(9):5425-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
  67. Inflammatory chemokine release of astrocytes in vitro is reduced by all-trans retinoic acid.
    Authors: van Neerven S, Regen T, Wolf D, Nemes A, Johann S, Beyer C, Hanisch UK, Mey J
    J. Neurochem., 2010;114(5):1511-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  68. Latent herpesvirus infection augments experimental pulmonary fibrosis.
    Authors: Vannella KM, Luckhardt TR, Wilke CA, van Dyk LF, Toews GB, Moore BB
    Am. J. Respir. Crit. Care Med., 2010;181(5):465-77.
    Species: Mouse
    Sample Types: Tissue Homogenates
  69. Reduction in dietary omega-6 polyunsaturated fatty acids: eicosapentaenoic acid plus docosahexaenoic acid ratio minimizes atherosclerotic lesion formation and inflammatory response in the LDL receptor null mouse.
    Authors: Wang S, Wu D, Matthan NR, Lamon-Fava S, Lecker JL, Lichtenstein AH
    Atherosclerosis, 2009;204(1):147-55.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  70. Modulation of macrophage infiltration and inflammatory activity by the phosphatase SHP-1 in virus-induced demyelinating disease.
    Authors: Christophi GP, Hudson CA, Panos M, Gruber RC, Massa PT
    J. Virol., 2009;83(2):522-39.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  71. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400.
    Species: Mouse
    Sample Types: BALF
  72. E-prostanoid 3 receptor deletion improves pulmonary host defense and protects mice from death in severe Streptococcus pneumoniae infection.
    Authors: Aronoff DM, Lewis C, Serezani CH, Eaton KA, Goel D, Phipps JC, Peters-Golden M, Mancuso P
    J. Immunol., 2009;183(4):2642-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  73. Matrix metalloproteinase-9 deficiency worsens lung injury in a model of bronchopulmonary dysplasia.
    Authors: Lukkarinen H, Hogmalm A, Lappalainen U, Bry K
    Am. J. Respir. Cell Mol. Biol., 2009;41(1):59-68.
    Species: Mouse
    Sample Types: Tissue Homogenates
  74. Scavenging roles of chemokine receptors: chemokine receptor deficiency is associated with increased levels of ligand in circulation and tissues.
    Authors: Cardona AE, Sasse ME, Liu L, Cardona SM, Mizutani M, Savarin C, Hu T, Ransohoff RM
    Blood, 2008;112(2):256-63.
    Species: Mouse
    Sample Types: Tissue Homogenates
  75. Central role of MyD88-dependent dendritic cell maturation and proinflammatory cytokine production to control Brucella abortus infection.
    Authors: Macedo GC, Magnani DM, Carvalho NB, Bruna-Romero O, Gazzinelli RT, Oliveira SC
    J. Immunol., 2008;180(2):1080-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  76. Surfactant protein D alters allergic lung responses in mice and human subjects.
    Authors: Brandt EB, Mingler MK, Stevenson MD, Wang N, Khurana Hershey GK, Whitsett JA, Rothenberg ME
    J. Allergy Clin. Immunol., 2008;121(5):1140-1147.e2.
    Species: Mouse
    Sample Types: BALF
  77. Distinct roles for IL-13 and IL-4 via IL-13 receptor alpha1 and the type II IL-4 receptor in asthma pathogenesis.
    Authors: Munitz A, Brandt EB, Mingler M, Finkelman FD, Rothenberg ME
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7240-5.
    Species: Mouse
    Sample Types: BALF
  78. Neurokinin A engages neurokinin-1 receptor to induce NF-kappaB-dependent gene expression in murine macrophages: implications of ERK1/2 and PI 3-kinase/Akt pathways.
    Authors: Sun J, Ramnath RD, Tamizhselvi R, Bhatia M
    Am. J. Physiol., Cell Physiol., 2008;295(3):C679-91.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  79. Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
    Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
    J. Neurosci., 2008;28(13):3277-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  80. Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
    Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
    Immunity, 2007;26(4):445-59.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  81. Infiltrating neutrophils induce allospecific CTL in response to immunization with apoptotic cells via MCP-1 production.
    Authors: Shiratsuchi Y, Iyoda T, Tanimoto N, Kegai D, Nagata K, Kobayashi Y
    J. Leukoc. Biol., 2007;81(2):412-20.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  82. Neurokinin-1 receptor antagonist treatment protects mice against lung injury in polymicrobial sepsis.
    Authors: Hegde A, Zhang H, Moochhala SM, Bhatia M
    J. Leukoc. Biol., 2007;82(3):678-85.
    Species: Mouse
    Sample Types: Plasma
  83. Mycobacterium tuberculosis antigens specifically modulate CCR2 and MCP-1/CCL2 on lymphoid cells from human pulmonary hilar lymph nodes.
    Authors: Arias MA, Jaramillo G, Lopez YP, Mejia N, Mejia C, Pantoja AE, Shattock RJ, Garcia LF, Griffin GE
    J. Immunol., 2007;179(12):8381-91.
    Species: Human
    Sample Types: Cell Culture Supernates
  84. CD8+ T Cells are required for inflammation and destruction in cigarette smoke-induced emphysema in mice.
    Authors: Maeno T, Houghton AM, Quintero PA, Grumelli S, Owen CA, Shapiro SD
    J. Immunol., 2007;178(12):8090-6.
    Species: Mouse
    Sample Types: BALF
  85. Osteopontin regulates hindlimb-unloading-induced lymphoid organ atrophy and weight loss by modulating corticosteroid production.
    Authors: Wang KX, Shi Y, Denhardt DT
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(37):14777-82.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  86. Differential involvement of TLR2 and TLR4 in host survival during pulmonary infection with Chlamydia pneumoniae.
    Authors: Rodriguez N, Wantia N, Fend F, Durr S, Wagner H, Miethke T
    Eur. J. Immunol., 2006;36(5):1145-55.
    Species: Mouse
    Sample Types: Tissue Homogenates
  87. Exogenous platelet-activating factor acetylhydrolase reduces mortality in mice with systemic inflammatory response syndrome and sepsis.
    Authors: Gomes RN, Bozza FA, Amancio RT, Japiassu AM, Vianna RC, Larangeira AP, Gouvea JM, Bastos MS, Zimmerman GA, Stafforini DM, Prescott SM, Bozza PT, Castro-Faria-Neto HC
    Shock, 2006;26(1):41-9.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  88. A functional comparison of canine and murine bone marrow derived cultured mast cells.
    Authors: Lin TY, London CA,
    Vet. Immunol. Immunopathol., 2006;114(3):320-34.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  89. Increased pulmonary responses to acute ozone exposure in obese db/db mice.
    Authors: Lu FL, Johnston RA, Flynt L, Theman TA, Terry RD, Schwartzman IN, Lee A, Shore SA
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;290(5):L856-65.
    Species: Mouse
    Sample Types: Serum
  90. Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
    Authors: Carpenter KJ, Hogaboam CM
    Infect. Immun., 2005;73(11):7198-207.
    Species: Mouse
    Sample Types: Tissue Homogenates
  91. Impaired inflammatory angiogenesis, but not leukocyte influx, in mice lacking TNFR1.
    Authors: Barcelos LS, Talvani A, Teixeira AS, Vieira LQ, Cassali GD, Andrade SP, Teixeira MM
    J. Leukoc. Biol., 2005;78(2):352-8.
    Species: Mouse
    Sample Types: Complex Sample Type

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Mouse CCL2/JE/MCP-1 DuoSet ELISA
By Anonymous on 04/02/2020
Application: Sample Tested: Serum and Plasma

We used this kit for the quantification of CCL2 in human serum and plasma. Works very well and well-described protocol.


Mouse CCL2/JE/MCP-1 DuoSet ELISA
By Anonymous on 09/03/2019
Application: Sample Tested: mouse BAL,Monocytes,Blood monocytes

Mouse CCL2/JE/MCP-1 DuoSet ELISA
By Anonymous on 12/11/2018
Application: Sample Tested: Serum and Plasma

Mouse CCL2/JE/MCP-1 DuoSet ELISA
By Anonymous on 07/13/2018
Application: Sample Tested: Brain tissue

Mouse CCL2/JE/MCP-1 DuoSet ELISA
By Anonymous on 10/19/2017
Application: Sample Tested: Cell Culture Supernates

Mouse CCL2/JE/MCP-1 DuoSet ELISA
By Anonymous on 10/06/2016
Application: Sample Tested: Cell culture supernatant

Mouse CCL2/JE/MCP-1 DuoSet ELISA
By Anonymous on 07/06/2016
Application: Sample Tested: Mouse BAL fluid