CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.
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Mouse CCL2/JE/MCP-1 DuoSet ELISA
R&D Systems | Catalog # DY479
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Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
3.91-250 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse
Mouse CCL2/JE/MCP-1 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
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Product Summary for Mouse CCL2/JE/MCP-1 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CCL2/JE. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse CCL2/JE/MCP-1 DuoSet ELISA
Mouse CCL2 / JE / MCP-1 ELISA Standard Curve
Kit Contents for Mouse CCL2/JE/MCP-1 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CCL2/JE/MCP-1
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
Additional CCL2/JE/MCP-1 Products
Product Documents for Mouse CCL2/JE/MCP-1 DuoSet ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CCL2/JE/MCP-1 DuoSet ELISA
For research use only
Citations for Mouse CCL2/JE/MCP-1 DuoSet ELISA
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Customer Reviews for Mouse CCL2/JE/MCP-1 DuoSet ELISA (19)
4.6 out of 5
19 Customer Ratings
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Sample Tested: Peritoneal macrophagesVerified Customer | Posted 10/05/2023
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Sample Tested: Colon tissueVerified Customer | Posted 09/11/2023
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Sample Tested: Colon tissueVerified Customer | Posted 05/19/2023
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Sample Tested: Cell culture supernatantVerified Customer | Posted 11/07/2022
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Sample Tested: Colon tissueVerified Customer | Posted 06/09/2022
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Sample Tested: Cell culture supernatantVerified Customer | Posted 04/13/2022
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Sample Tested: Serum and PlasmaVerified Customer | Posted 03/11/2022Used this kit for quantifying mouse MCP-1 in serum samples, could see the detection but not strong in our samples. Standard curve looks good.
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Sample Tested: Colon tissue and SerumVerified Customer | Posted 01/19/2022stable and precise
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Sample Tested: mouse cell line conditioned mediaVerified Customer | Posted 12/21/2021
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Sample Tested: Serum and PlasmaVerified Customer | Posted 04/02/2020We used this kit for the quantification of CCL2 in human serum and plasma. Works very well and well-described protocol.
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Sample Tested: mouse BAL, Monocytes and Blood monocytesVerified Customer | Posted 09/03/2019
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Sample Tested: Serum and PlasmaVerified Customer | Posted 12/11/2018
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Sample Tested: Plasma and peritoneal fluidVerified Customer | Posted 11/21/2018
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Sample Tested: Brain tissueVerified Customer | Posted 07/13/2018
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Sample Tested: Breast cancer cellsVerified Customer | Posted 05/04/2018
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Sample Tested: Pancreatic cancer cellsVerified Customer | Posted 04/26/2018
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Sample Tested: Cell Culture SupernatesVerified Customer | Posted 10/19/2017
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Sample Tested: Cell culture supernatantVerified Customer | Posted 10/06/2016
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Sample Tested: Mouse BAL fluidVerified Customer | Posted 07/06/2016
There are no reviews that match your criteria.
Protocols
View specific protocols for Mouse CCL2/JE/MCP-1 DuoSet ELISA (DY479):
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
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- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
