Mouse CCL20/MIP-3 alpha Antibody MAB7601-100: R&D Systems

Mouse CCL20/MIP-3 alpha Antibody

(8 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL20/MIP-3 alpha in ELISAs. Does not cross-react with recombinant human (rh) CCL19, rhCCL20, recombinant mouse (rm) CCL3, 4, 9, 19, rmCXCL2, or recombinant rat CCL20.
  • Source
    Monoclonal Rat IgG1 Clone # 114908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL20/MIP-3 alpha
    Ala28-Met97
    Accession # O89093
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse CCL20/MIP-3 alpha Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CCL20/MIP‑3 alpha Antibody (Catalog # MAB7601)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CCL20/MIP‑3 alpha Biotinylated Antibody (Catalog # BAF760)
  • ELISA Standard
     
    Recombinant Mouse CCL20/MIP-3 alpha Protein (Catalog # 760-M3)
  • Neutralization
    Measured by its ability to neutralize CCL20/MIP‑3 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR6. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL20/MIP‑3 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL20/MIP‑3 alpha and Neutralization by Mouse CCL20/MIP‑3 alpha Antibody. Recombinant Mouse CCL20/MIP‑3 alpha (Catalog #
760-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL20/MIP‑3 alpha (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL20/MIP‑3 alpha Monoclonal Antibody (Catalog # MAB7601). The ND50 is typically 3-15 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL20/MIP-3 alpha

MIP-3 alpha, also known as LARC (Liver and Activation-regulated Chemokine) and as Exodus, is one of many novel beta chemokines identified through bioinformatics. Mouse MIP-3 alpha cDNA encodes a 97 amino acid residue precursor protein with a 27 aa residue putative signal peptide that is predicted to be cleaved to form the 70 amino acid (aa) residue mature secreted protein. MIP-3 alpha is distantly related to other beta chemokines (20-28% aa sequence identity). Mouse MIP-3 alpha shares approximately 71% and 63% amino acid sequence homology with rat and human MIP-3 alpha, respectively.

MIP-3 alpha has been shown to be expressed predominantly in lymph nodes, appendix, PBL, fetal liver, fetal lung, and epithelial cells of intestinal tissues. The expression of MIP-3 alpha is strongly up-regulated by inflammatory signals and down-regulated by the anti-inflammatory cytokine IL-10. Synthetic or recombinant MIP-3 alpha has been shown to be chemotactic for lymphocytes and dendritic cells, and inhibits proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha has now been shown to be a unique functional ligand for CCR-6 (previously referred to as GPR-CY4, CKR-L3, or STRL22 orphan receptor), a chemokine receptor that is selectively and highly expressed in human dendritic cells derived from CD34+ cord blood precursors.

  • References:
    1. Baba, M. et al. (1997) J. Biol. Chem. 272:14893.
    2. Hromas, R. et al. (1997) Blood 89:3315.
    3. Greaves, D.R. et al. (1997) J. Exp. Med. 186: 857.
    4. Tanaka, Y. et al. (1999) Eur. J. Immunol. 29:633.
  • Entrez Gene IDs:
    6364 (Human); 20297 (Mouse); 29538 (Rat)
  • Alternate Names:
    beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
  2. The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages.
    Authors: Boyle S, Faulkner J, McColl S, Kochetkova M
    Mol Cancer, 2015;14(0):115.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  3. CCR6 is required for epidermal trafficking of gammadelta-T cells in an IL-23-induced model of psoriasiform dermatitis.
    Authors: Mabuchi T, Singh T, Takekoshi T, Jia G, Wu X, Kao M, Weiss I, Farber J, Hwang S
    J Invest Dermatol, 2013;133(1):164-71.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  4. Tc17 Cells Mediate Vaccine Immunity against Lethal Fungal Pneumonia in Immune Deficient Hosts Lacking CD4+ T Cells.
    Authors: Nanjappa SG, Heninger E, Wuthrich M, Gasper DJ, Klein BS
    PLoS Pathog., 2012;8(7):e1002771.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. CCL20/CCR6 blockade enhances immunity to RSV by impairing recruitment of DC.
    Authors: Kallal LE, Schaller MA, Lindell DM, Lira SA, Lukacs NW
    Eur. J. Immunol., 2010;40(4):1042-52.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  6. Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection.
    Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR
    J. Immunol., 2009;182(5):3055-62.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008;112(2):362-73.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  8. The chemokine receptor CCR6 is an important component of the innate immune response.
    Authors: Wen H, Hogaboam CM, Lukacs NW, Cook DN, Lira SA, Kunkel SL
    Eur. J. Immunol., 2007;37(9):2487-98.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Isotype Controls
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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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