Mouse CCL21/6Ckine Antibody

Catalog # Availability Size / Price Qty
AF457
AF457-SP
Mouse CCL21/6Ckine Antibody in Data
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Product Details
Citations (41)
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Reviews (2)

Mouse CCL21/6Ckine Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross reactivity with recombinant rat CCL21/6Ckine is observed and less than 15% cross-reactivity with recombinant human CCL21/6Ckine is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse CCL21/6Ckine
Ser24-Gly133
Accession # P84444
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C)
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize CCL21/6Ckine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse CCL21/6Ckine.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody. View Larger

Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody. Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL21/6Ckine (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457). The ND50is typically 0.5-2.5 µg/mL.

Immunohistochemistry CCL21/6Ckine in Mouse Thymus. View Larger

CCL21/6Ckine in Mouse Thymus. CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Intracellular Staining by Flow Cytometry Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry. View Larger

Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL21/6Ckine

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21-33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta /ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines. Mature rat CCL21 shares 84% aa sequence identity with mouse CCL21.

Entrez Gene IDs
6366 (Human); 18829 (Mouse); 298006 (Rat)
Alternate Names
6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4

Product Datasheets

Citations for Mouse CCL21/6Ckine Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. YAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells
    Authors: SY Choi, H Bae, SH Jeong, I Park, H Cho, SP Hong, DH Lee, CK Lee, JS Park, SH Suh, J Choi, MJ Yang, JY Jang, L Onder, JH Moon, HS Jeong, RH Adams, JM Kim, B Ludewig, JH Song, DS Lim, GY Koh
    Nat Commun, 2020;11(1):519.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  2. Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas
    Authors: X Jia, F Gábris, Ó Jacobsen, G Bedics, B Botz, Z Helyes, Z Kellermaye, D Vojkovics, G Berta, N Nagy, Z Jakus, P Balogh
    J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Single-Cell Analysis Reveals Heterogeneity of High Endothelial Venules and Different Regulation of Genes Controlling Lymphocyte Entry to Lymph Nodes
    Authors: K Veerman, C Tardiveau, F Martins, J Coudert, JP Girard
    Cell Rep, 2019;26(11):3116-3131.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp
    Authors: HW Cheng, L Onder, M Novkovic, C Soneson, M Lütge, N Pikor, E Scandella, MD Robinson, JI Miyazaki, A Tersteegen, U Sorg, K Pfeffer, T Rülicke, T Hehlgans, B Ludewig
    Nat Commun, 2019;10(1):1739.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Neuroinflammation-induced lymphangiogenesis near the cribriform plate contributes to drainage of CNS-derived antigens and immune cells
    Authors: M Hsu, A Rayasam, JA Kijak, YH Choi, JS Harding, SA Marcus, WJ Karpus, M Sandor, Z Fabry
    Nat Commun, 2019;10(1):229.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators
    Authors: CA Thomson, SA van de Pav, M Stakenborg, E Labeeuw, G Matteoli, AM Mowat, RJB Nibbs
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Regulation of T cell afferent lymphatic migration by targeting LT?R-mediated non-classical NF?B signaling
    Authors: W Piao, Y Xiong, K Famulski, CC Brinkman, L Li, N Toney, C Wagner, V Saxena, T Simon, JS Bromberg
    Nat Commun, 2018;9(1):3020.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19
    Authors: L Liu, L Zhao, Y Yang, J Gao, C Hu, B Guo, B Zhu
    Oncol Lett, 2018;16(4):5013-5019.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Brief Exposure of Skin to Near-Infrared Laser Modulates Mast Cell Function and Augments the Immune Response
    Authors: Y Kimizuka, W Katagiri, JJ Locascio, A Shigeta, Y Sasaki, M Shibata, K Morse, RF Sîrbulescu, M Miyatake, P Reeves, M Suematsu, J Gelfand, T Brauns, MC Poznansky, K Tsukada, S Kashiwagi
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal-to-Noise Ratio and Dependent on GRK6
    Authors: J Schwarz, V Bierbaum, K Vaahtomeri, R Hauschild, M Brown, I de Vries, A Leithner, A Reversat, J Merrin, T Tarrant, T Bollenbach, M Sixt
    Curr. Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: IHC
  11. Increased migration of antigen presenting cells to newly-formed lymphatic vessels in transplanted kidneys by glycol-split heparin
    Authors: DT Talsma, K Katta, M Boersema, S Adepu, A Naggi, G Torri, C Stegeman, G Navis, H van Goor, JL Hillebrand, S Yazdani, J van den Bo
    PLoS ONE, 2017;12(6):e0180206.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Functional Assay
  12. Role of SIRP? in Homeostatic Regulation of T Cells and Fibroblastic Reticular Cells in the Spleen
    Authors: D Respatika, Y Saito, K Washio, S Komori, T Kotani, H Okazawa, Y Murata, T Matozaki
    Kobe J Med Sci, 2017;63(1):E22-E29.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration
    Authors: C Colin Brinkman
    Nat Commun, 2016;7(0):12021.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Functional Assay
  14. Collecting lymphatic vessel permeability facilitates adipose tissue inflammation and distribution of antigen to lymph node-homing adipose tissue dendritic cells.
    Authors: Kuan E, Ivanov S, Bridenbaugh E, Victora G, Wang W, Childs E, Platt A, Jakubzick C, Mason R, Gashev A, Nussenzweig M, Swartz M, Dustin M, Zawieja D, Randolph G
    J Immunol, 2015;194(11):5200-10.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Structural and functional features of central nervous system lymphatic vessels.
    Authors: Louveau A, Smirnov I, Keyes T, Eccles J, Rouhani S, Peske J, Derecki N, Castle D, Mandell J, Lee K, Harris T, Kipnis J
    Nature, 2015;523(7560):337-41.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. The Schlemm's canal is a VEGF-C/VEGFR-3-responsive lymphatic-like vessel.
    Authors: Aspelund A, Tammela T, Antila S, Nurmi H, Leppanen V, Zarkada G, Stanczuk L, Francois M, Makinen T, Saharinen P, Immonen I, Alitalo K
    J Clin Invest, 2014;124(9):3975-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  17. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
    Authors: Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P
    Infect Immun, 2014;82(8):3098-112.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. CLEC-2 is required for development and maintenance of lymph nodes.
    Authors: Benezech C, Nayar S, Finney B, Withers D, Lowe K, Desanti G, Marriott C, Watson S, Caamano J, Buckley C, Barone F
    Blood, 2014;123(20):3200-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. CXCR4 is dispensable for T cell egress from chronically inflamed skin via the afferent lymph.
    Authors: Geherin, Skye A, Wilson, R Paul, Jennrich, Silke, Debes, Gudrun F
    PLoS ONE, 2014;9(4):e95626.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. TWEAK promotes peritoneal inflammation.
    Authors: Sanz A, Aroeira L, Bellon T, del Peso G, Jimenez-Heffernan J, Santamaria B, Sanchez-Nino M, Blanco-Colio L, Lopez-Cabrera M, Ruiz-Ortega M, Egido J, Selgas R, Ortiz A
    PLoS ONE, 2014;9(3):e90399.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers.
    Authors: Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F
    Clin Cancer Res, 2014;20(8):2147-58.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Increased expression of the homeostatic chemokines CCL19 and CCL21 in clinical and experimental Rickettsia conorii infection.
    Authors: Astrup E, Ranheim T, Damas J, Davi G, Santilli F, Jensenius M, Vitale G, Aukrust P, Olano J, Otterdal K
    BMC Infect Dis, 2014;14(0):70.
  23. Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
    Authors: Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D
    Am J Pathol, 2014;184(5):1577-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Remodeling of the lymphatic vasculature during mouse mammary gland morphogenesis is mediated via epithelial-derived lymphangiogenic stimuli.
    Authors: Betterman K, Paquet-Fifield S, Asselin-Labat M, Visvader J, Butler L, Stacker S, Achen M, Harvey N
    Am J Pathol, 2012;181(6):2225-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  25. Exposure to Ionizing Radiation Induces the Migration of Cutaneous Dendritic Cells by a CCR7-Dependent Mechanism.
    Authors: Cummings, Ryan J, Gerber, Scott A, Judge, Jennifer, Ryan, Julie L, Pentland, Alice P, Lord, Edith M
    J Immunol, 2012;189(9):4247-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. NK cells regulate CD8+ T cell priming and dendritic cell migration during influenza A infection by IFN-gamma and perforin-dependent mechanisms.
    J Immunol, 2012;189(5):2099-109.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs.
    Authors: Rehm A, Mensen A, Schradi K, Gerlach K, Wittstock S, Winter S, Buchner G, Dorken B, Lipp M, Hopken UE
    Blood, 2011;118(4):1020-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  28. Induction of targeted cell migration by cutaneous administration of a DNA vector encoding a biologically active chemokine CCL21.
    Authors: Jalili A, Pashenkov M, Kriehuber E, Wagner C, Nakano H, Stingl G, Wagner SN
    J. Invest. Dermatol., 2010;130(6):1611-23.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Ontogeny of stromal organizer cells during lymph node development.
    Authors: Benezech C, White A, Mader E, Serre K, Parnell S, Pfeffer K, Ware CF, Anderson G, Caamano JH
    J. Immunol., 2010;184(8):4521-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  31. Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.
    Authors: Grabner R, Lotzer K, Dopping S, Hildner M, Radke D, Beer M, Spanbroek R, Lippert B, Reardon CA, Getz GS, Fu YX, Hehlgans T, Mebius RE, van der Wall M, Kruspe D, Englert C, Lovas A, Hu D, Randolph GJ, Weih F, Habenicht AJ
    J. Exp. Med., 2009;206(1):233-48.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  32. Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines.
    Authors: St John AL, Abraham SN
    Nat. Med., 2009;15(11):1259-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  33. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
    Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
    J. Immunol., 2009;182(11):6851-60.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  34. Macrophage mannose receptor on lymphatics controls cell trafficking.
    Authors: Marttila-Ichihara F, Turja R, Miiluniemi M, Karikoski M, Maksimow M, Niemela J, Martinez-Pomares L, Salmi M, Jalkanen S
    Blood, 2008;112(1):64-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. The chemokine receptor CCR7 mediates corneal antigen-presenting cell trafficking.
    Authors: Jin Y, Shen L, Chong EM
    Mol. Vis., 2007;13(0):626-34.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  36. CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo.
    Authors: Worbs T, Mempel TR, Bolter J, von Andrian UH, Forster R
    J. Exp. Med., 2007;204(3):489-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  37. The role of lymphoid tissue inducer cells in splenic white pulp development.
    Authors: Withers DR, Kim MY, Bekiaris V, Rossi SW, Jenkinson WE, Gaspal F, McConnell F, Caamano JH, Anderson G, Lane PJ
    Eur. J. Immunol., 2007;37(11):3240-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  38. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006;203(12):2763-77.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  39. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2005;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  40. Local delivery of recombinant vaccinia virus expressing secondary lymphoid chemokine (SLC) results in a CD4 T-cell dependent antitumor response.
    Authors: Flanagan K, Glover RT, Horig H, Yang W, Kaufman HL
    Vaccine, 2004;22(21):2894-903.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
    Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
    J. Immunol., 2000;164(5):2650-9.
    Species: Rat
    Sample Types: In Vivo
    Applications: Neutralization

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Mouse CCL21/6Ckine Antibody
By Anonymous on 04/13/2018
Application: IHC Sample Tested: Serum Species: Mouse

Mouse CCL21/6Ckine Antibody
By Anonymous on 04/07/2016
Application: ICC/IF Sample Tested: Small intestine tissue Species: Mouse

Red staining is CCL21