Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CCL21/6Ckine Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CCL21/6Ckine
Ser24-Gly133
Accession # P84444

Specificity

Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross reactivity with recombinant rat CCL21/6Ckine is observed and less than 15% cross-reactivity with recombinant human CCL21/6Ckine is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CCL21/6Ckine Antibody

Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody.

Chemotaxis Induced by CCL21/6Ckine and Neutralization by Mouse CCL21/6Ckine Antibody.

Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL21/6Ckine (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457). The ND50 is typically 0.5-2.5 µg/mL.
CCL21/6Ckine antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr).

CCL21/6Ckine in Mouse Thymus.

CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of CCL21/6Ckine antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse CCL21/6Ckine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF457, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Mouse CCL21/6Ckine by Immunohistochemistry

Detection of Mouse CCL21/6Ckine by Immunohistochemistry

Afferent lymph vessels in chronically inflamed skin express CXCL12.Immunofluorescence staining of frozen sections of chronically inflamed skin (21 days after inflammation elicited by subcutaneous injection of CFA) for CXC12 and CCL21 by LYVE-1+ lymph vessels using DAPI counterstaining. One representative image of lymph vessels analyzed in 5 mice is shown. Scale bars, 10 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24752354), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CCL21/6Ckine Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: D3 mouse embryonic stem cell line fixed with paraformaldehyde and permeabilized with saponin.

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C)

Neutralization

Measured by its ability to neutralize CCL21/6Ckine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse CCL21/6Ckine.

Reviewed Applications

Read 3 reviews rated 4.7 using AF457 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL21/6Ckine

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21-33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta /ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines. Mature rat CCL21 shares 84% aa sequence identity with mouse CCL21.

Alternate Names

6Ckine, exodus-2, SCYA21, SLC, TCA-4

Entrez Gene IDs

6366 (Human); 18829 (Mouse); 298006 (Rat)

Gene Symbol

CCL21

UniProt

P84444

Additional CCL21/6Ckine Products

Product Documents for Mouse CCL21/6Ckine Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CCL21/6Ckine Antibody

For research use only

Citations for Mouse CCL21/6Ckine Antibody

Customer Reviews for Mouse CCL21/6Ckine Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mouse CCL21/6Ckine Antibody
    Name: Leslie Priddy
    Application: Immunohistochemistry
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 04/13/2018
  • Mouse CCL21/6Ckine Antibody
    Name: Sonia Rehal
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Small intestine tissue
    Species: Mouse
    Verified Customer | Posted 04/07/2016
    Red staining is CCL21
    Mouse CCL21/6Ckine Antibody AF457
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 24006262
    Species: Mouse
    Verified Customer | Posted 01/08/2015

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Protocols

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