Detects mouse CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse (rm) CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22 or 24, recombinant human (rh) CCL2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26, or recombinant rat (rr) CCL20 is observed.
Monoclonal Rat IgG2B Clone # 89818
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse CCL25/TECK Gln24-Asn144 Accession # O35903.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Mouse CCL25/TECK (Catalog # 481-TK) under non-reducing conditions only
Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR9. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 1.6 µg/mL Recombinant Mouse CCL25/TECK.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL25/TECK and Neutralization by Mouse CCL25/TECK Antibody. Recombinant Mouse CCL25/TECK (Catalog # 481‑TK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL25/TECK (1.6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL25/TECK Monoclonal Antibody (Catalog # MAB481). The ND50 is typically 1.5 ‑ 6.0 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein. Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant mouse CCL25 has been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes (1).
Vicari, A.P. et al. (1997) Immunity 7:291.
Entrez Gene IDs:
6370 (Human); 20300 (Mouse)
C-C motif chemokine 25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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