Mouse CCL25/TECK Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL25/TECK in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse (rm) CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22 or 24, recombinant human (rh) CCL2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or 26, or recombinant rat (rr) CCL20 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 89818
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL25/TECK
    Gln24-Asn144
    Accession # O35903.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CCL25/TECK (Catalog # 481-TK)
    under non-reducing conditions only
  • Neutralization
    Measured by its ability to neutralize CCL25/TECK-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR9. The Neutralization Dose (ND50) is typically 1.5-6.0 µg/mL in the presence of 1.6 µg/mL Recombinant Mouse CCL25/TECK.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL25/TECK and Neutralization by Mouse CCL25/TECK Antibody. Recombinant Mouse CCL25/TECK (Catalog # 481‑TK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR9 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL25/TECK (1.6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL25/TECK Monoclonal Antibody (Catalog # MAB481). The ND50 is typically 1.5 ‑ 6.0 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL25/TECK

CCL25 (thymus-expressed chemokine) is a novel CC chemokine that is distantly related (twenty-some % amino acid sequence identity) to other CC chemokines. Mouse CCL25 cDNA encodes a 144 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 121 residue mature protein.  Mouse CCL25 shares 49% amino acid sequence identity with human CCL25. The expresssion of human and mouse CCL25 was shown to be highly restricted to the thymus and small intestine. Although dendritic cells have been demonstrated to be the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. The gene for mouse CCL25 has been mapped to chromosome 8 rather than chromosome 11 where many mouse CC chemokines are clustered. Recombinant mouse CCL25 has been shown to be chemotactic for activated macrophages, dendritic cells and thymocytes (1).

  • References:
    1. Vicari, A.P. et al. (1997) Immunity 7:291.
  • Entrez Gene IDs:
    6370 (Human); 20300 (Mouse)
  • Alternate Names:
    C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012;42(5):1250-60.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. A subset of interleukin-21+ chemokine receptor CCR9+ T helper cells target accessory organs of the digestive system in autoimmunity.
    Authors: McGuire HM, Vogelzang A, Ma CS, Hughes WE, Silveira PA, Tangye SG, Christ D, Fulcher D, Falcone M, King C
    Immunity, 2011;34(4):602-15.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization.
    Authors: Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y
    Blood, 2006;108(8):2531-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Isotype Controls
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Rat IgG2B Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2B Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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