Mouse CCL5/RANTES Antibody

Name: Mouse CCL5/RANTES Antibody
Brand: R&D Systems
SKU: MAB478-500
Rating: 4.7 (3 reviews)

Catalog #: MAB478
18 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB478-SP
MAB478-100
MAB478-500

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Citations (18)

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Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCL5/RANTES Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCL5/RANTES in direct ELISAs. Shows 50% cross-reactivity with recombinant human (rh) CCL5, and no cross-reactivity with recombinant feline CCL5, rhCCL3, recombinant mouse (rm) CCL3, rmCCL4, rhCCL4, rhCCL14, or rhCCL18.

Source

Monoclonal Rat IgG_2A Clone # 53405

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse CCL5/RANTES
Ser24-Ser91
Accession # P30882

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.1-0.5 µg/mL in the presence of 0.025 µg/mL Recombinant Mouse CCL5/RANTES.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CCL5/RANTES in Mouse Splenocytes. CCL5/RANTES was detected in immersion fixed mouse splenocytes using Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization

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Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. Recombinant Mouse CCL5/RANTES (Catalog # 478-MR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.025 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478). The ND₅₀ is typically 0.1-0.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% amino acid (aa) sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of pro-inflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

References

Schall, T.J. et al. (1990) Nature 347:669.
Bacon, K.B. et al. (1995) Science 269:1727.
Fischer, F.R. et al. (2001) J. Immunol. 167:1637.
Schall, T.J. et al. (1992) Eur. J. Immunol. 22:1477.
Heeger, P. et al. (1992) Kidney Int. 41:220.
Appay, V. and S.L. Rowland-Jones (2001) Trends Immunol. 22:83.
Levy, J.A. (2009) J. Immunol. 182:3945.
Randolph-Habecker, J.R. et al. (2002) Cytokine 19:37.
DeVico, A.L. and R.C. Gallo (2004) Nat. Rev. Microbiol. 2:401.
Proost, P. et al. (1998) J. Biol. Chem. 273:7222.
Appay, V. et al. (1999) J. Biol. Chem. 274:27505.
Proudfoot, A.E.I. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1885.
von Hundelshausen, P. et al. (2005) Blood 105:924.
von Hundelshausen, P. et al. (2001) Circulation 103:1772.
Zernecke, A. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1897.
Baltus, T. et al. (2003) Blood 102:1985.
Soria, G. and A. Ben-Baruch (2008) Cancer Lett. 267:271.

Entrez Gene IDs

6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)

Alternate Names

CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse CCL5/RANTES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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Transcriptional control of pancreatic cancer immunosuppression by metabolic enzyme CD73 in a tumor-autonomous and -autocrine manner
Authors: Tang, T;Huang, X;Lu, M;Zhang, G;Han, X;Liang, T;
Nature communications
Species: Rat
Sample Types: In Vivo
Applications: In Vivo
IL-3 orchestrates ulcerative colitis pathogenesis by controlling the development and the recruitment of splenic reservoir neutrophils
Authors: Bénard, A;Mittelstädt, A;Klösch, B;Glanz, K;Müller, J;Schoen, J;Nüse, B;Brunner, M;Naschberger, E;Stürzl, M;Mattner, J;Muñoz, LE;Sohn, K;Grützmann, R;Weber, GF;
Cell reports
Species: Mouse
Sample Types:
Applications: In Vivo
Runx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity
Authors: W Seo, K Shimizu, S Kojo, A Okeke, T Kohwi-Shig, SI Fujii, I Taniuchi
Nat Commun, 2020-03-26;11(1):1562.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Hepatospecific ablation of p38alpha MAPK governs liver regeneration through modulation of inflammatory response to CCl4-induced acute injury
Authors: M Fortier, M Cadoux, N Boussetta, S Pham, R Donné, JP Couty, C Desdouets, S Celton-Mor
Sci Rep, 2019-10-10;9(1):14614.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Nitrosporeusine analogue ameliorates Chandipura virus induced inflammatory response in CNS via NF?b inactivation in microglia
Authors: AK Verma, TS Waghmare, GR Jachak, SC Philkhana, DS Reddy, A Basu
PLoS Negl Trop Dis, 2018-07-12;12(7):e0006648.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control
Authors: JP Böttcher, E Bonavita, P Chakravart, H Blees, M Cabeza-Cab, S Sammicheli, NC Rogers, E Sahai, S Zelenay, C Reis E Sou
Cell, 2018-02-08;172(5):1022-1037.e14.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
The HIV co-receptor CCR5 regulates osteoclast function
Authors: JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura
Nat Commun, 2017-12-20;8(1):2226.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
CCL5 Promotes Resolution-Phase Macrophage Reprogramming in Concert with the Atypical Chemokine Receptor D6 and Apoptotic Polymorphonuclear Cells
Authors: M Aswad, S Assi, S Schif-Zuck, A Ariel
J. Immunol., 2017-07-03;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway
J Neuroinflammation, 2016-08-30;13(1):209.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, Neutralization
Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases.
Authors: Sottnik J, Dai J, Zhang H, Campbell B, Keller E
Cancer Res, 2015-04-08;75(11):2151-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes.
Authors: Herz J, Pardo J, Kashkar H, Schramm M, Kuzmenkina E, Bos E, Wiegmann K, Wallich R, Peters PJ, Herzig S, Schmelzer E, Kronke M, Simon MM, Utermohlen O
Nat. Immunol., 2009-06-14;10(7):761-8.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection.
Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR
J. Immunol., 2009-03-01;182(5):3055-62.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
J. Exp. Med., 2008-08-11;205(9):2075-84.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Joint capsule treatment with enkephalin-encoding HSV-1 recombinant vector reduces inflammatory damage and behavioural sequelae in rat CFA monoarthritis.
Authors: Lu Y, McNearney TA, Wilson SP, Yeomans DC, Westlund KN
Eur. J. Neurosci., 2008-03-01;27(5):1153-65.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Authors: Fujita H, Asahina A, Komine M, Tamaki K
Cell. Immunol., 2007-05-15;245(2):70-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
Proc. Natl. Acad. Sci. U.S.A., 2007-02-05;104(7):2319-24.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
Authors: Mosmann T
Eur. J. Immunol., 2004-06-01;34(6):1617-26.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development

NKT cell-derived RANTES recruits APCs and CD8+ T cells to the spleen during the generation of regulatory T cells in tolerance.
Authors: Faunce DE, Stein-Streilein J
J. Immunol., 2002-07-01;169(1):31-8.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization