Mouse CCL9/10/MIP‑1 gamma Biotinylated Antibody

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Product Details
Citations (2)
Supplemental Products

Mouse CCL9/10/MIP‑1 gamma Biotinylated Antibody Summary

Species Reactivity
Detects mouse CCL9/10/MIP-1 gamma in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant viral (rv) MIP-I, rvMIP-II, recombinant human (rh) MIP-1 alpha, rhMIP-1 beta, rhMIP-1δ, rhMIP-3 alpha, rhMIP-3 beta, recombinant mouse (rm) MIP‑1 alpha, rmMIP-1 beta, recombinant rat MIP-3 alpha, and rmMIP-3 beta is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse CCL9/10/MIP-1 gamma (R&D Systems, Catalog # 463-MG)
Accession # Q3U9T8
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse CCL9/10/MIP‑1 gamma (Catalog # 463-MG)

Mouse CCL9/10/MIP-1 gamma Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse CCL9/10/MIP‑1 gamma Antibody (Catalog # MAB463)

Standard: Recombinant Mouse CCL9/10/MIP-1 gamma Protein (Catalog # 463-MG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL9/10/MIP-1 gamma

Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1 - 3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3 - 7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71 - 73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full-length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).

  1. Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
  2. Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
  3. Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
  4. Haelens, A. et al. (1996) Immunobiology 195:499.
  5. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
  6. Youn, B-S. et al. (1995) J. Immunol. 155:2661.
  7. Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.
Entrez Gene IDs
20308 (Mouse)
Alternate Names
CCF18; CCL9/10; MIP1 gamma; MIP-1 gamma

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Citations for Mouse CCL9/10/MIP‑1 gamma Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  2. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Yang L, Mosmann T
    Eur. J. Immunol., 2004;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development


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