Mouse CCL9/10/MIP-1 gamma Antibody Summary
Accession # Q3U9T8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL9/10/MIP‑1 gamma and Neutral-ization by Mouse CCL9/10/MIP‑1 gamma Antibody. Recombinant Mouse CCL9/10/MIP-1 gamma (Catalog # 463-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL9/10/MIP-1 gamma (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL9/10/ MIP-1 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF463). The ND50 is typically 0.3-1.0 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL9/10/MIP-1 gamma
Mouse CCL9/10 (also named MIP-1 gamma and MRP-2) is an 11 kDa, secreted, monomeric polypeptide that belongs to the beta (or CC) intercrine family of chemokines (1‑3). Based on its activity and amino acid (aa) sequence, it is further classified as a member of the NC6 or six cysteine-containing CC subfamily of chemokines (2, 4, 5). This subfamily contains four N-terminally extended chemokines, two human (CCL15 and CCL23) and two mouse (CCL9 and CCL10). Within this subfamily, there are no human-to-rodent interspecies orthologs. Mouse CCL9/10 is synthesized as a 122 aa precursor that contains a 21 aa signal sequence and a 101 aa mature region with six cysteines. As noted, the mature region has an expanded N-terminus relative to other CC family members, and it forms a third intrachain disulfide bond with its two extra cysteines (3‑7). Mouse CCL9/10 is 75% aa identical to rat CCL9/10 (8). Chemokines are known to undergo proteolytic processing to generate multiple isoforms. NC6 chemokines are usually only marginally active at full length, but are converted to highly active forms upon N-terminal truncation. Mature CCL9, in the presence of inflammatory fluids, is naturally truncated by 28, 29 or 30 aa at the N-terminus, generating a highly active, 8 kDa, 71‑73 aa CCR1 ligand. In contrast, other CCR1 ligands, CCL3/MIP-1 alpha and CCL5/RANTES, lose their potency when proteolytically processed. CCL9/10 is constitutively secreted, and circulates as a full‑length molecule. Any onset of inflammation with subsequent enzyme release may act on local NC6 chemokines, generating early, potent leukocyte chemoattractants (5, 7).
- Zlotnik, A. and O. Yoshie (2000) Immunity 12:121.
- Zlotnik, A. et al. (1999) Crit. Rev. Immunol. 19:1
- Mohamadzadeh, M. et al. (1996) J. Immunol. 156:3102.
- Haelens, A. et al. (1996) Immunobiology 195:499.
- Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
- Youn, B-S. et al. (1995) J. Immunol. 155:2661.
- Poltorak, A.N. et al. (1995) J. Inflamm. 45:207.
Citations for Mouse CCL9/10/MIP-1 gamma Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Chemokine CXCL16 mediates acinar cell necrosis in cerulein induced acute pancreatitis in mice
Authors: Y Sakuma, Y Kodama, T Eguchi, N Uza, Y Tsuji, M Shiokawa, T Maruno, K Kuriyama, Y Nishikawa, Y Yamauchi, M Tsuda, T Ueda, T Matsumori, T Morita, T Tomono, N Kakiuchi, A Mima, Y Sogabe, S Marui, T Kuwada, A Okada, T Watanabe, H Nakase, T Chiba, H Seno
Sci Rep, 2018;8(1):8829.
Sample Types: Whole Tissue
Changes in gene expression of pial vessels of the blood brain barrier during murine neurocysticercosis.
Authors: Mishra, Pramod K, Teale, Judy M
PLoS Negl Trop Dis, 2013;7(3):e2099.
Sample Types: Whole Tissue
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010;120(3):907-23.
Sample Types: Whole Cells
Applications: Flow Cytometry
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