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Mouse CCR3 Alexa Fluor® 700-conjugated Antibody

FAB729N 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB729N-100UG
Mouse CCR3 Alexa Fluor® 700-conjugated Antibody in Flow Cytometry
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Product Details
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.
Source
Monoclonal Rat IgG2A Clone # 83101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse CCR3
Met1-Phe359
Accession # P51678
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CCR3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB729N) and Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F). Quadrant markers were set based on control antibody staining (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

Product Datasheets

Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

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Reviews for Mouse CCR3 Alexa Fluor® 700-conjugated Antibody

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Mouse CCR3 Alexa Fluor® 700-conjugated Antibody
By Anonymous on 08/14/2016
Application: Flow Sample Tested: Bronchoalveolar lavage fluid Species: Mouse
Flow Cytometry Mouse CCR3 Alexa Fluor® 700-conjugated Antibody FAB729N