Mouse CCR3 Alexa Fluor® 700-conjugated Antibody

(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.
  • Source
    Monoclonal Rat IgG2A Clone # 83101
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with mouse CCR3
    Met1-Phe359
    Accession # P51678
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mousesplenocytes were stained with Rat Anti-Mouse CCR3 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB729N) and Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F). Quadrant markers were set based on control antibody staining (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR3
CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.
  • Entrez Gene IDs:
    1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
  • Alternate Names:
    b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse CCR3 Alexa Fluor® 700-conjugated Antibody FAB729N
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 Flow Mouse Anonymous 08/14/2016
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Flow Cytometry Mouse CCR3 Alexa Fluor® 700-conjugated Antibody FAB729N
Flow Cytometry: Mouse CCR3 Alexa Fluor® 700-conjugated Antibody [FAB729N]

Summary

ApplicationFlow Cytometry
Sample TestedBronchoalveolar lavage fluid
SpeciesMouse

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