Mouse CCR3 Alexa Fluor® 700-conjugated Antibody

Name: Mouse CCR3 Alexa Fluor® 700-conjugated Antibody
Brand: R&D Systems
Rating: 3 (1 reviews)

FAB729N 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB729N-025
FAB729N-100

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Detection of CCR3 in Mouse Splenocytes by Flow Cytometry.

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Mouse CCR3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR3. Specifically stains mouse CCR3 transfectants but not the parental cell lines. Does not cross‑react with Y3 cells transfected with human CCR3.

Source

Monoclonal Rat IgG_2A Clone # 83101

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CCR3
Met1-Phe359
Accession # P51678

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700

Applications

Recommended Concentration

Sample

Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CCR3 in Mouse Splenocytes by Flow Cytometry. Mousesplenocytes were stained with Rat Anti-Mouse CCR3 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB729N) and Rat Anti-Mouse Gr‑1/Ly‑6G Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1037F). Quadrant markers were set based on control antibody staining (Catalog # IC006N). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane receptor that is activated by several different chemokines. It is expressed on subsets of granulocytes, monocytes, T cells and epithelial cells.

Entrez Gene IDs

1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)

Alternate Names

b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841