CCR4 (C-C chemokine receptor 4) is a 42 kDa member of the GPCR #1 family of transmembrane proteins. In rodent, functional CCR4 is expressed on fully differentiated CD4+ Th2 cells, invariant CD4+ NKT cells, and neurons. It is the receptor for CCL17/TARC, CCL22/MDC and CKLF1, and responds to these ligands by initiating chemotaxis. Mouse CCR4 is a 7-transmembrane protein that is 360 amino acids (aa) in length. It contains a 39 aa N-terminal extracellular domain, and a 52 aa C-terminal cyoplasmic tail that shows a poly-Ser motif between aa 343-346. Over aa sequences 1-39, 99-111, 176-205 and 268-284 collectively, mouse CCR4 shares 85% and 93% aa identity with human and rat CCR4, respectively.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse CCR4
Met1-Glu39, Ala99-Lys111, Ser176-Ile205, Val268-Tyr284
Accession # P51680
Met1-Glu39, Ala99-Lys111, Ser176-Ile205, Val268-Tyr284
Accession # P51680
Specificity
Detects mouse CCR4 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse CCR4 Antibody
Detection of Mouse CCR4 by Western Blot.
Western Blot shows lysates of EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/ml of Sheep Anti-Mouse CCR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5207) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR4 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 8.
Applications for Mouse CCR4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: EL‑4 mouse lymphoblast cell line
Sample: EL‑4 mouse lymphoblast cell line
Reviewed Applications
Read 3 reviews rated 4 using AF5207 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCR4
Alternate Names
CD194
Entrez Gene IDs
Gene Symbol
CCR4
UniProt
Additional CCR4 Products
Product Documents for Mouse CCR4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CCR4 Antibody
For research use only
Related Research Areas
Citations for Mouse CCR4 Antibody
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Customer Reviews for Mouse CCR4 Antibody (3)
4 out of 5
3 Customer Ratings
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Application: Western BlotSample Tested: Lung tissueSpecies: MouseVerified Customer | Posted 11/13/2024
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Application: Simple WesternSample Tested: Aortic smooth muscle cellsSpecies: MouseVerified Customer | Posted 12/05/2021
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Application: Western BlotSample Tested: HASMC human aortic smooth muscle cellsSpecies: HumanVerified Customer | Posted 06/13/2017dilution: 1:800 secondary antibody dil: 1: 6000 I use to get few non-specific bands.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
