Mouse CCR7 Antibody

Catalog #: MAB3477
9 Citations Datasheet / COA / SDS

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MAB3477
MAB3477-SP

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Citations (9)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse CCR7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CCR7.

Source

Monoclonal Rat IgG_2A Clone # 4B12

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

RBC-2H3 cells expressing mouse CCR7
Accession # NP_031745

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-reported

Spitzer, M. et al. (2015) Science 349: 1259425. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Neutralization

Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR7. The Neutralization Dose (ND₅₀) is typically 2-10 µg/mL in the presence of 25 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CCR7 in Mouse Splenocytes. CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization

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Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCR7 Antibody. Recombinant Mouse CCL19/MIP-3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP-3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND₅₀ is typically 2-10 µg/mL.

Flow Cytometry

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Detection of CCR7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG_2AIsotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR7

CCR7 (Chemokine Receptor 7; also CD197) is a 7 transmembrane (7TM) G protein-coupled chemokine receptor for the homeostatic chemokines CCL19/MIP-3 beta and CCL21/6Ckine. CCL19 and CCL21 are constitutively expressed by high endothelial venule epithelial cells or fibroblastic reticular cells in secondary lymphoid organs. CCR7 is upregulated on dendritic cells, naïve and memory T cells, Treg, NK cells, and B cells following inflammatory stimulation. Its expression enables the function of immune cell trafficking to and retention in regional lymph nodes for expansion of the adaptive immune response. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.

Entrez Gene IDs

1236 (Human); 12775 (Mouse); 287673 (Rat)

Alternate Names

BLR2; BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7; CMKBR7chemokine (C-C) receptor 7; EBI1; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse CCR7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Ligand-competent fractalkine receptor is expressed on exosomes
Authors: EJ Park, PK Myint, MG Appiah, P Worawattan, J Inprasit, O Prajuabjin, ZY Soe, A Gaowa, E Kawamoto, M Shimaoka
Biochemistry and Biophysics Reports, 2021;26(0):100932.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Helicobacter pylori-induced Rev-erb&alpha fosters gastric bacteria colonization by impairing host innate and adaptive defense
Authors: FY Mao, YP Lv, CJ Hao, YS Teng, YG Liu, P Cheng, SM Yang, W Chen, T Liu, QM Zou, R Xie, JY Xu, Y Zhuang
Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Contribution of Heparan Sulphate Binding in CCL21-Mediated Migration of Breast Cancer Cells
Authors: I Del Molino, A Meeson, K Cooke, MI Malki, B Barron-Mil, JA Kirby, S Ali
Cancers, 2021;13(14):.
Lymphangiogenesis in kidney and lymph node mediates renal inflammation and fibrosis
Authors: G Pei, Y Yao, Q Yang, M Wang, Y Wang, J Wu, P Wang, Y Li, F Zhu, J Yang, Y Zhang, W Yang, X Deng, Z Zhao, H Zhu, S Ge, M Han, R Zeng, G Xu
Sci Adv, 2019;5(6):eaaw5075.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
FOXO1 regulates dendritic cell activity through ICAM-1 and CCR7.
Authors: Dong G, Wang Y, Xiao W, Pacios Pujado S, Xu F, Tian C, Xiao E, Choi Y, Graves D
J Immunol, 2015;194(8):3745-55.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC
TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.
Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J
Oncogene, 2015;35(6):748-60.
CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma.
Authors: Irino, Tomoyuki, Takeuchi, Hiroya, Matsuda, Sachiko, Saikawa, Yoshiro, Kawakubo, Hirofumi, Wada, Norihito, Takahashi, Tsunehir, Nakamura, Rieko, Fukuda, Kazumasa, Omori, Tai, Kitagawa, Yuko
BMC Cancer, 2014;14(0):291.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development
Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
Authors: Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P
Infect Immun, 2014;82(8):3098-112.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Direct B cell stimulation by dendritic cells in a mouse model of lupus.
Authors: Wan S, Zhou Z, Duan B, Morel L
Arthritis Rheum., 2008;8(0):1741-50.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry