Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
0.25 µg/106 cells
Spitzer, M. et al. (2015) Science 349: 1259425. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR7. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 25 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CCR7 in Mouse Splenocytes. CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCR7 Antibody. Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND50 is typically 2-10 µg/mL.
Detection of CCR7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR7 is a 7TM G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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