Mouse CCR7 Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCR7.
  • Source
    Monoclonal Rat IgG2A Clone # 4B12
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    RBC-2H3 cells expressing mouse CCR7
    Accession # NP_031745
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-reported
    Spitzer, M. et al. (2015) Science 349: 1259425. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR7. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 25 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CCR7 in Mouse Splenocytes. CCR7 was detected in immersion fixed non-stimulated mouse splenocytes using 25 µg/mL Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCR7 Antibody. Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477). The ND50 is typically 2-10 µg/mL.
Detection of CCR7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CCR7 Monoclonal Antibody (Catalog # MAB3477) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCR7

CCR7 is a 7TM G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7.

  • Entrez Gene IDs:
    1236 (Human); 12775 (Mouse); 287673 (Rat)
  • Alternate Names:
    BLR2C-C chemokine receptor type 7; C-C CKR-7; CC-CKR-7; CCR7; CCR-7; CD197 antigen; CD197; CDw197CC chemokine receptor 7; chemokine (C-C motif) receptor 7; CMKBR7chemokine (C-C) receptor 7; EBI1EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr virus induced G-protein coupled receptor; Epstein-Barr virus-induced G-protein coupled receptor 1; EVI1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 beta receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.
    Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J
    Oncogene, 2015;35(6):748-60.
  2. FOXO1 regulates dendritic cell activity through ICAM-1 and CCR7.
    Authors: Dong G, Wang Y, Xiao W, Pacios Pujado S, Xu F, Tian C, Xiao E, Choi Y, Graves D
    J Immunol, 2015;194(8):3745-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IF
  3. CC-Chemokine receptor CCR7: a key molecule for lymph node metastasis in esophageal squamous cell carcinoma.
    Authors: Irino, Tomoyuki, Takeuchi, Hiroya, Matsuda, Sachiko, Saikawa, Yoshiro, Kawakubo, Hirofumi, Wada, Norihito, Takahashi, Tsunehir, Nakamura, Rieko, Fukuda, Kazumasa, Omori, Tai, Kitagawa, Yuko
    BMC Cancer, 2014;14(0):291.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Development
  4. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
    Authors: Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P
    Infect Immun, 2014;82(8):3098-112.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Direct B cell stimulation by dendritic cells in a mouse model of lupus.
    Authors: Wan S, Zhou Z, Duan B, Morel L
    Arthritis Rheum., 2008;8(0):1741-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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