Mouse CCR7 APC‑conjugated Antibody

Detection of CCR7 in Mouse CD4⁺Splenocytes by Flow Cytometry.

Mouse CD4⁺splenocytes were stained with Rat Anti-Mouse CCR7 APC-conjugated Monoclonal Antibody (Catalog # FAB3477A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Mouse CCR7 by Flow Cytometry

B6 and CD69−/− CD4 T cells differ in the expression of naïve and memory surface cell markers.Cells were isolated from the spleen (spl), mesenteric lymph nodes (MLN), small intestinal lamina propria (siLP), colonic lamina propria (cLP) and blood (bld) of non-treated B6 and CD69−/− mice and analyzed by flow cytometry. The surface expression of CD44 (A), CD103 (B), CD62L (C), CCR-7 (D), CCR-9 (E) and alpha 4 beta 7 integrin (F) by CD4 T cells were analysed. Graphs represent mean (± SEM) of CD4 T cell fraction expressing the indicated molecule for four mice per each strain per tissue. Black bars represent data for B6 and white bars for CD69−/− cells. *p≤0.05. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23776480), licensed under a CC-BY license. Not internally tested by R&D Systems.