Detection of CD11c in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse MHC class II (I‑A/I‑E) Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB6118F) and either (A) Hamster Anti-Mouse CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB69501A) or (B) Hamster IgG Allophycocyanin Control. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Integrin alpha X subunit, also known as CD11c, is a 150 kDa type I
transmembrane protein that noncovalently heterodimerizes with the beta 2
subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement
receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages,
dendritic cells, hairy cell leukemias and some other leukocyte subsets.
The 1097 aa mouse alpha X extracellular domain shares 71% and 87% amino acid
(aa) identity with human and rat alpha X, respectively. One potential alpha X
isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are
shared with alpha M beta 2/CD11b/CD18 (complement iC3b, ICAMs, vWF and fibrinogen)
while others (osteopontin, Thy-1, plasminogen, heparin) are unique.
Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates
proliferation, degranulation, chemotactic migration, and phagocytosis of
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