Mouse CD11c APC-conjugated Antibody

FAB69501A has been discontinued. View all CD11c products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin alpha X/CD11c.
  • Source
    Monoclonal Hamster IgG Clone # N418
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse spleen dendritic cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD11c in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse MHC class II (I‑A/I‑E) Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB6118F) and either (A) Hamster Anti-Mouse CD11c APC‑conjugated Monoclonal Antibody (Catalog # FAB69501A) or (B) Hamster IgG Allophycocyanin Control. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD11c
The Integrin alpha X subunit, also known as CD11c, is a 150 kDa type I transmembrane protein that noncovalently heterodimerizes with the beta 2 subunit (CD18) to form alpha X/ beta 2, also known as p150/p95 and complement receptor type 4 (CR4). Integrin alpha X beta 2 is expressed on macrophages, dendritic cells, hairy cell leukemias and some other leukocyte subsets. The 1097 aa mouse alpha X extracellular domain shares 71% and 87% amino acid (aa) identity with human and rat alpha X, respectively. One potential alpha X isoform is truncated at aa 828. Some adhesion partners of alpha X beta 2 are shared with alpha M beta 2/CD11b/CD18 (complement iC3b, ICAMs, vWF and fibrinogen) while others (osteopontin, Thy-1, plasminogen, heparin) are unique. Unlike alpha M beta 2, it is not constitutively active. alpha X beta 2 adhesion mediates proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles.
  • References:
    1. Metlay, J.P. et al. (1990) J. Exp. Med. 171:1753.
  • Entrez Gene IDs:
    3687 (Human); 16411 (Mouse); 64350 (Rat)
  • Alternate Names:
    95 alpha chain; 95; 95, alpha subunit; CD11 antigen-like family member C; CD11c antigen; CD11c; CD11Cleu M5, alpha subunit; Integrin alpha X; integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); ITGAX; Leu M5; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150; leukocyte surface antigen p150; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain; p150,95 alpha; SLEB6
Related Research Areas

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