Mouse CD200 R1 PE-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects mouse CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human CD200 R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD200 R1
    Accession # BAE42266
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD200 R1 in MC/9‑2 Mouse Cell Line by Flow Cytometry. MC/9‑2 mouse mast cell line was stained with Goat Anti-Mouse CD200 R1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2554P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD200 R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1‑3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5‑7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13‑15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

  • References:
    1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
    2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. 
    3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. 
    4. Wright, G.J. et al. (2003) J. Immunol. 171:3034. 
    5. Shiratori, I. et al. (2005) J. Immunol. 175:4441. 
    6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. 
    7. Fallarino, F. et al. (2004) J. Immunol. 173:3748. 
    8. Wright, G.J. et al. (2001) Immunology 102:173. 
    9. Hoek, R.M. et al. (2000) Science 290:1768.
    10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
    11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
    12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
    13. Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.
    14. Zhang, S. et al. (2004) J. Immunol. 173:6786.
    15. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
    16. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
  • Long Name:
    CD200 Receptor 1
  • Entrez Gene IDs:
    131450 (Human); 57781 (Mouse)
  • Alternate Names:
    CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Airway epithelial cells condition dendritic cells to express multiple immune surveillance genes.
    Authors: Rate A, Bosco A, McKenna K, Holt P, Upham J
    PLoS ONE, 2012;7(9):e44941.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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