Mouse CD200R1 Antibody

Catalog # Availability Size / Price Qty
MAB2554
MAB2554-SP

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Product Details
Citations (1)
FAQs
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Mouse CD200R1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD200 R1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD200 R1 is observed.
Source
Monoclonal Rat IgG2A Clone # 330502
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD200 R1
Thr26-Pro238
Accession # BAE42266
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CD200 R1 Fc Chimera (Catalog # 2554-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD200R1

CD200 R1, also known as OX-2 receptor, is a 90 kDa, type I transmembrane protein that belongs to the immunoglobulin superfamily. CD200 R1 is important in the regulation of myeloid cell activity (1‑3). The mouse CD200 R1 cDNA encodes a 326 aa precursor that includes a 25 aa signal sequence, a 213 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 67 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain (4). Within the ECD, mouse CD200 R1 shares 56% and 70% aa sequence identity with human and rat CD200 R1, respectively. The ECD of mouse CD200 R1 shares 69%, 38%, 79%, and 83% aa sequence identity with the ECD of CD200 R2, 3, 4, and a CD200 R-like molecule, respectively. CD200 R1 is expressed primarily on mast cells, basophils, macrophages, and dendritic cells, (5‑7) while its ligand, CD200, is widely distributed (8). Disruption of this receptor-ligand pair by knockout of the CD200 gene leads to increased macrophage number and activation, plus a predisposition to autoimmune disorders (9). Association of CD200 with CD200 R1 takes place between their respective N-terminal Ig-like domains (10). The CD200 R-like molecules may interact differently with CD200 (11, 12). The cytoplasmic domain of CD200 R1 contains two non-ITIM tyrosine residues which are required for propagating its inhibitory signals (13‑15). CD200 R-like molecules, in contrast, are potentially activating receptors by means of their association with DAP12 (4, 16).

References
  1. Rosenblum, M.D. et al. (2006) J. Dermatol. Sci. 41:165.
  2. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483. 
  3. Barclay, A.N. et al. (2002) Trends Immunol. 23:285. 
  4. Wright, G.J. et al. (2003) J. Immunol. 171:3034. 
  5. Shiratori, I. et al. (2005) J. Immunol. 175:4441. 
  6. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348. 
  7. Fallarino, F. et al. (2004) J. Immunol. 173:3748. 
  8. Wright, G.J. et al. (2001) Immunology 102:173. 
  9. Hoek, R.M. et al. (2000) Science 290:1768.
  10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
  11. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
  12. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
  13. Zhang, S. and J.H. Phillips (2006) J. Leukoc. Biol. 79:363.
  14. Zhang, S. et al. (2004) J. Immunol. 173:6786.
  15. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
  16. Voehringer, D. et al. (2004) J. Biol. Chem. 279:54117.
Long Name
CD200 Receptor 1
Entrez Gene IDs
131450 (Human); 57781 (Mouse); 102139962 (Cynomolgus Monkey)
Alternate Names
CD200 R1; CD200 receptor 1; CD200R1; CD200RMOX2Rcell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; CRTR2; HCRTR2; MOX2 receptor; MOX2R; OX2RCD200 cell surface glycoprotein receptor

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Citation for Mouse CD200R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell-endothelium interactions.
    Authors: Ko, Ya-Chen, Chien, Hsiung-F, Jiang-Shieh, Ya-Fen, Chang, Chiu-Yun, Pai, Man-Hui, Huang, Jian-Pei, Chen, Hui-Min, Wu, Ching-Hs
    J Anat, 2009;214(1):183-95.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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