Mouse CD27/TNFRSF7 APC-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD27/TNFRSF7

CD27 is a 50-55 kDa lymphocyte-specific member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF) and is also known as TNFRSF7. Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region. Over aa 21-182, mouse CD27 shares approximately 84% and 60% amino acid sequence identity with rat and human CD27, respectively. CD27 exists as a homodimer on the cell surface due to an extracellular disulfide bond in the membrane-proximal region. A soluble 32 kDa form of CD27 is also produced during the immune response and is found in various body fluids. CD27 is expressed on subsets of NK, T and B cells. This includes δ zeta T cells, CD25⁺ T regs, immature CD11b^lo NK cells, and resting plus effector CD8⁺ T cells. The expression of CD27 is upregulated upon T cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells. CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. Ligation of CD27 on B cells has been shown to both inhibit the terminal differentiation of activated mouse B cells into plasma cells.

Product Datasheets

Related Research Areas

• B Cell Markers
• Cancer Stem Cell Markers
• Co-stimulatory and Co-inhibitory Molecules
• gamma delta T Cells
• Isolation, Culture, and Identification of Natural Killer Cells
• Regulation of Apoptosis by TNF Superfamily Members
• Regulation of B Cell Activation and Homeostasis by TNF Superfamily Members
• Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members
• Regulation of T Cell Co-stimulation by TNF Superfamily Members
• Regulatory T Cells (Tregs)
• Search Immune Checkpoint Targets
• TNF Superfamily
• TNF Superfamily Receptors