Detection of CD27/TNFRSF7 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD27/TNFRSF7 APC‑conjugated Monoclonal Antibody (Catalog # FAB5741A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD27 is a 50-55 kDa lymphocyte-specific member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF) and is also known as TNFRSF7. Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region. Over aa 21-182, mouse CD27 shares approximately 84% and 60% amino acid sequence identity with rat and human CD27, respectively. CD27 exists as a homodimer on the cell surface due to an extracellular disulfide bond in the membrane-proximal region. A soluble 32 kDa form of CD27 is also produced during the immune response and is found in various body fluids. CD27 is expressed on subsets of NK, T and B cells. This includes δ zeta T cells, CD25+ T regs, immature CD11blo NK cells, and resting plus effector CD8+ T cells. The expression of CD27 is upregulated upon T cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells. CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. Ligation of CD27 on B cells has been shown to both inhibit the terminal differentiation of activated mouse B cells into plasma cells.
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