Mouse CD27/TNFRSF7 APC-conjugated Antibody

FAB5741A has been discontinued. View all CD27/TNFRSF7 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD27 in direct ELISAs and Western blots. In Western blots; does not cross-react with recombinant human (rh) CD27, recombinant mouse (rm) CD40, rmGITR, rhDR6, rhTRAIL R4, rmHVEM, rmCD30, rmRANK, rmFas, rmTRAIL R2, rhTRADD, rm4-1BB, rmLT beta R, rmOPG, rmNGFR, rmDR3, rmBAFF R, rhTRAIL R3, rmTAJ, rmEDAR, rmOX-40, rmTWEAK R, rmTNF R1, rhRELT,  or rmTRAILR1.
  • Source
    Monoclonal Rat IgG2A Clone # 137915
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD27/TNFRSF7
    Thr21-Arg182
    Accession # P41272
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD27/TNFRSF7 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD27/TNFRSF7 APC‑conjugated Monoclonal Antibody (Catalog # FAB5741A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD27/TNFRSF7
CD27 is a 50-55 kDa lymphocyte-specific member of the Tumor Necrosis Factor Receptor Superfamily (TNFRSF) and is also known as TNFRSF7. Mouse CD27 cDNA encodes a 250 amino acid (aa) residue type I transmembrane protein with a 20 aa putative signal peptide, a 162 aa extracellular region containing three TNFR cysteine-rich repeats, a 21 aa transmembrane domain and a 47 aa cytoplasmic region. Over aa 21-182, mouse CD27 shares approximately 84% and 60% amino acid sequence identity with rat and human CD27, respectively. CD27 exists as a homodimer on the cell surface due to an extracellular disulfide bond in the membrane-proximal region. A soluble 32 kDa form of CD27 is also produced during the immune response and is found in various body fluids. CD27 is expressed on subsets of NK, T and B cells. This includes δ zeta T cells, CD25+ T regs, immature CD11blo NK cells, and resting plus effector CD8+ T cells. The expression of CD27 is upregulated upon T cell activation. Although CD27 appears to be a marker for human memory B cells, it is only expressed in a small population of mouse B cells in germinal centers and at sites of B cell stimulation, suggesting that mouse CD27 may be a marker for activated B cells. CD27 interacts with CD27 ligand (also named CD70 and TNFSF7), which is a member of the TNF ligand superfamily. Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. Ligation of CD27 on B cells has been shown to both inhibit the terminal differentiation of activated mouse B cells into plasma cells.
  • Entrez Gene IDs:
    939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)
  • Alternate Names:
    CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
Related Research Areas

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