Mouse CD6 Antibody

Catalog # Availability Size / Price Qty
MAB727
MAB727-SP
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Mouse CD6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD6 in direct ELISAs and Western blots. Does not cross-react with human CD6.
Source
Monoclonal Rat IgG2A Clone # 96123
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD6
Leu18-Gly396
Accession # Q61003
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot <PSTRONG/STRONG/P> View Larger

Detection of Mouse CD6 by Western Blot.Western blot shows lysates of mouse lymph node or thymus in reducing (R) or non-reducing (NR) sample buffer. PVDF membrane was probed with 2 µg/mL of Mouse CD6 Monoclonal Antibody (Catalog # MAB727) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CD6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of CD6 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD6 Monoclonal Antibody (Catalog # MAB727) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) and Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD6

CD6 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily. CD6 is a type I membrane glycoprotein and contains three extracellular SRCR domains. CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. Mouse CD6 is a 626 amino acid (aa) protein with a 24 aa signal sequence, a 372 aa extracellular domain, and a 204 aa cytoplasmic region. The 668 aa human homolog has also been identified. The human and murine proteins share 70% aa identity over their full-lengths.

The role of CD6 has not been fully elucidated. However, it appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co-stimulatory molecule. Additionally, anti-CD6 monoclonal antibody has been used as an immunosuppressive agent for patients undergoing kidney or bone marrow allograft rejection. It has also been used to remove CD6+ T cells from donor bone marrow prior to allogenic bone marrow transplantation. Other studies have demonstrated an adhesive role for CD6, it has been demonstrated to bind the activated leukocyte cell adhesion molecule (ALCAM, CD166). CD6/ALCAM interactions have been postulated to play a role in thymocyte development. Additionally, the presence of ALCAM on neuronal cells may provide a mechanism of interaction between CD6+ T cell and ALCAM+ neuronal cells. Phosphorylation of the CD6 molecule appears to play a role in CD6-mediated signal transduction. Serine and threonine residues become hyperphosphorylated and tyrosine residues become phosphorylated when T cells are activated with anti-CD6 mAb in conjunction with PMA, anti-CD2, or anti-CD3 mAb. The CD6 intracellular domain contains regions that can interact with SH2 or SH3 containing proteins. However, the signaling pathways have not been elucidated.

References
  1. Gangemi, R. et al. (1989) J. Immunol. 143:2439.
  2. Aruffo, A. et al. (1991) J. Exp. Med. 174:949.
  3. Swack, J.A. et al. (1991) J. Biol. Chem. 266:7137.
  4. Robinson, W.H. et al. (1995) Eur. J. Immunol. 25:2765.
  5. Whitney, G. et al. (1995) Mol. Immunol. 32:89.
  6. Starling, G.C. et al. (1996) Eur. J. Immunol. 26:738.
  7. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  8. Swack, J.A. et al. (1989) Mol. Immunol. 26:1037.
  9. Pawelec, G. and H.J. Buhring (1991) Human Immunol. 31:165.
  10. Osorio, L.M. et al. (1995) Cell Immunol. 166:44.
  11. Robinson, W.H. et al. (1995) J. Immunol. 155:4739.
  12. Singer, N.G. et al. (1996) Immunology 88:537.
  13. Aruffo, A. et al. (1997) Immunol. Today 18:498.
Entrez Gene IDs
923 (Human); 12511 (Mouse); 102143944 (Cynomolgus Monkey)
Alternate Names
CD6 antigenFLJ44171; CD6 molecule; CD6; T12; Tp120; TP120T-cell differentiation antigen CD6

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