Mouse CD63 PE-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD63 in direct ELISAs.
  • Source
    Monoclonal Rat IgG1 Clone # 446703
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with mouse CD63
    Met1-Met238
    Accession # P41731
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CD63 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse CD63 PE‑conjugated Monoclonal Antibody (Catalog # FAB5417P). Quadrant markers were set based on control antibody staining (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD63

CD63, also known as LAMP-3 or ME491 (melanoma-associated antigen), is a 30-60 kDa member of the tetraspanin superfamily of protein trafficking molecules. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation, is also present on the plasma membrane. Interaction of CD63 with other membrane proteins or adaptors regulates cell activities such as adhesion, migration and degranulation. Extracellular regions of mouse CD63 share 94% and 67% amino acid sequence identity with rat and human CD63, respectively.

  • Entrez Gene IDs:
    967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)
  • Alternate Names:
    CD63 antigen (melanoma 1 antigen); CD63 antigen; CD63 molecule; CD63; Granulophysin; Lamp-3; Lysosomal-associated membrane protein 3; ME491; melanoma 1 antigen; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; MLA1lysosome-associated membrane glycoprotein 3; Ocular melanoma-associated antigen; OMA81H; tetraspanin-30; Tspan30; tspan-30; TSPAN30granulophysin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 PE-conjugated Antibody

Ctrl IC005P  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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