Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry. CTLL‑2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2386F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a 23-34 kDa member of the NK receptor family. It is a homodimeric type II transmembrane protein containing a Ca++-independent C-type lectin domain that appears very early following leukocyte activation. Leukocytes known to express CD69 include T cells, B cells, Neutrophils, NK cells and monocytes. Although CD69 ligation has been described as promoting inflammation, some studies suggest that CD69 regulates inflammation by interfering with Th17 differentiation. This may be accomplished by Galectin-1 expression on DC binding to CD69 expressed on CD4+ T cells. Over amino acids (aa) 64-199, mouse CD69 shares 78% and 57% aa sequence identity with rat and human CD69, respectively.
Entrez Gene IDs:
969 (Human); 12515 (Mouse)
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60
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