Mouse CD69 Fluorescein-conjugated Antibody Summary
Accession # P37217
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry. CTLL‑2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2386F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a 23-34 kDa member of the NK receptor family. It is a homodimeric type II transmembrane protein containing a Ca++-independent C-type lectin domain that appears very early following leukocyte activation. Leukocytes known to express CD69 include T cells, B cells, Neutrophils, NK cells and monocytes. Although CD69 ligation has been described as promoting inflammation, some studies suggest that CD69 regulates inflammation by interfering with Th17 differentiation. This may be accomplished by Galectin-1 expression on DC binding to CD69 expressed on CD4+ T cells. Over amino acids (aa) 64-199, mouse CD69 shares 78% and 57% aa sequence identity with rat and human CD69, respectively.
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