Mouse CD69 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2386F
Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry.
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Mouse CD69 Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.
Source
Monoclonal Rat IgG2B Clone # 310106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD69
Gly64-Arg199
Accession # P37217
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry. View Larger

Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry. CTLL‑2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2386F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD69

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a 23-34 kDa member of the NK receptor family. It is a homodimeric type II transmembrane protein containing a Ca++-independent C-type lectin domain that appears very early following leukocyte activation. Leukocytes known to express CD69 include T cells, B cells, Neutrophils, NK cells and monocytes. Although CD69 ligation has been described as promoting inflammation, some studies suggest that CD69 regulates inflammation by interfering with Th17 differentiation. This may be accomplished by Galectin-1 expression on DC binding to CD69 expressed on CD4+ T cells. Over amino acids (aa) 64-199, mouse CD69 shares 78% and 57% aa sequence identity with rat and human CD69, respectively.

Entrez Gene IDs
969 (Human); 12515 (Mouse)
Alternate Names
activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60

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