Mouse CD69 Fluorescein-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD69 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 310106
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD69
    Gly64-Arg199
    Accession # P37217
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD69 in CTLL‑2 Mouse Cell Line by Flow Cytometry. CTLL‑2 mouse cytotoxic T cell line was stained with Rat Anti-Mouse CD69 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2386F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD69
CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a 23-34 kDa member of the NK receptor family. It is a homodimeric type II transmembrane protein containing a Ca++-independent C-type lectin domain that appears very early following leukocyte activation. Leukocytes known to express CD69 include T cells, B cells, Neutrophils, NK cells and monocytes. Although CD69 ligation has been described as promoting inflammation, some studies suggest that CD69 regulates inflammation by interfering with Th17 differentiation. This may be accomplished by Galectin-1 expression on DC binding to CD69 expressed on CD4+ T cells. Over amino acids (aa) 64-199, mouse CD69 shares 78% and 57% aa sequence identity with rat and human CD69, respectively.
  • Entrez Gene IDs:
    969 (Human); 12515 (Mouse)
  • Alternate Names:
    activation inducer molecule (AIM/CD69); Activation inducer molecule; AIM; BL-AC/P26; CD69 antigen (p60, early T-cell activation antigen); CD69 antigen; CD69 molecule; CD69; CLEC2CC-type lectin domain family 2 member C; C-type lectin domain family 2, member C; EA1; EA-1; early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leu23; Leukocyte surface antigen Leu-23; MLR-3; p60
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Fluorescein-conjugated Antibody

Ctrl IC013F 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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