Mouse CD8 alpha MAb (Clone 53-6.7)

Name: Mouse CD8 alpha MAb (Clone 53-6.7)
Brand: R&D Systems
SKU: MLDP4

Catalog #: MLDP4 Datasheet / COA / SDS

Newer Version Available: MAB116

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Discontinued Product

MLDP4 has been discontinued and is replaced by MAB116.

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Mouse CD8 alpha MAb (Clone 53-6.7) Summary

Species Reactivity

Mouse

Specificity

This antibody has been shown to react with both the alpha and alpha  chains of mouse CD8 (Ly-2 or Lyt-2), an antigen co-receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells.² CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells.^3,4 CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells,⁴ intestinal intrathymic lymphocytes^6,7 and dendritic cells.^8,9

Source

Monoclonal Rat IgG2a Clone # 53-6.7

Label

Unconjugated

Applications

Recommended Concentration

Sample

Cell depletion

This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete CD8+ cells from 1 x 10⁹ bone marrow cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Cell Depletion

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Cell Depletion

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Figure 1:Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, CD11b/MAC-1 alpha, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems, Catalog # MAB1356; dotted histogram) in bone marrow-progenitor cells after enrichment using the mouse lineage panel and bead depletion.

Cell Depletion

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Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2