Mouse CD8 alpha Antibody

Catalog #: MAB11723 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11723-100
MAB11723-SP

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Detection of Mouse CD8 alpha by Western Blot.

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Mouse CD8 alpha Antibody Summary

Species Reactivity

Mouse

Specificity

Detects recombinant mouse CD8alpha protein in Direct ELISA.

Source

Monoclonal Rat IgG Clone # 1104518

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived mouse CD8
Lys28-Tyr196
Accession # P01731

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

Mouse thymus tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse CD8 alpha by Western Blot. Western Blot shows lysates of mouse thymus tissue. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB11723) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CD8 alpha at approximately 30 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References

Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
Hayakawa, K. et al. (1994) Science 263:1131.
MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
Rocha, B. et al. (1992) Immunol. Today 13:449.
Wang, J. and J.R. Klein (1994) Science 265:1860.
Vermec, D. et al. (1992) J. Exp. Med. 176:47.
Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Alternate Names

CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2