Mouse CDNF Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CDNF in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human CDNF is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 560108
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CDNF
    Leu27-Leu187
    Accession # Q8CC36
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CDNF in Mouse Brain. CDNF was detected in perfusion fixed frozen sections of mouse brain using Rat Anti-Mouse CDNF Monoclonal Antibody (Catalog # MAB5187) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to neuronal processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CDNF
CDNF (conserved dopamine neurotrophic factor), also called Armetl1 (arginine-rich, mutated in early stage tumors-like 1), is a 17‑19 kDa secreted protein that shares 52% amino acid (aa) identity with mouse MANF (mesencephalic-astrocyte-derived neurotrophic factor), also called Armet (1). The Armet designation is not preferred, because the proteins as translated are not actually arginine-rich (1). However, both CDNF and MANF have a high proportion of charged residues, a pattern of eight cysteines shown to form intramoleculular disulfide bonds, and a C-terminal endoplasmic reticulum retention signal (shown to function in MANF) (1‑3). The mouse CDNF cDNA encodes a 187 aa protein with a 24 aa signal sequence and a 163 mature sequence (1). Mature mouse CDNF shares 80%, 87%, 83% and 82% aa identity with human, rat, equine and bovine CDNF, respectively. Although CDNF mRNA and protein are expressed in pre and postnatal mouse brain, they are mostly abundant in adult heart, skeletal muscle and testis. Transcripts within the postnatal mouse brain are concentrated in the hippocampus, thalamus, corpus callosum and optic nerve (1). Like MANF and GDNF, CDNF promotes survival of dopaminergic neurons in vitro (1, 4). In a rat Parkinson's disease model, CDNF also promotes rescue and restoration of dopaminergic neurons in vivo (1).
  • References:
    1. Lindholm, P. et al. (2007) Nature 448:73.
    2. Mizobuchi, N. et al. (2007) Cell Struct. Funct. 32:41.
    3. Raykhel, I. et al. (2007) J. Cell Biol. 179:1193.
    4. Petrova, P. et al. (2003) J. Mol. Neurosci. 20:173.
  • Long Name:
    Conserved Dopamine Neurotrophic Factor
  • Entrez Gene IDs:
    441549 (Human); 227526 (Mouse); 361276 (Rat)
  • Alternate Names:
    arginine-rich, mutated in early stage tumors-like 1; ARMETL1; ARMET-like protein 1; CDNF; cerebral dopamine neurotrophic factor; Conserved dopamine neurotrophic factor
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