Mouse Chemerin Antibody

Catalog # Availability Size / Price Qty
Chemotaxis Induced by Chemerin and Neutralization by Mouse Chemerin Antibody.
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Product Details
Citations (10)
Supplemental Products

Mouse Chemerin Antibody Summary

Species Reactivity
Detects mouse Chemerin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human Chemerin is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse Chemerin
Accession # Q9DD06
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Chemerin (Catalog # 2325-CM)
Measured by its ability to neutralize Chemerin-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human Chem R23. The Neutralization Dose (ND50) is typically 3-9 µg/mL in the presence of 100 ng/mL Recombinant Mouse Chemerin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by Chemerin and Neutralization by Mouse Chemerin Antibody. View Larger

Chemotaxis Induced by Chemerin and Neutralization by Mouse Chemerin Antibody. Recombinant Mouse Chemerin (Catalog # 2325-CM) chemo-attracts the BaF3 mouse pro-B cell line transfected with human Chem R23 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Chemerin (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Chemerin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2325). The ND50 is typically 3-9 µg/mL.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chemerin

Mouse Chemerin, also known as Tazarotene-induced Gene-2 (TIG2), is a new, but distant member of the cystatin superfamily (1-3). Members of this superfamily contain at least two intrachain disulfide bonds and an alpha -helical structure over a distance of about 100 amino acids (2, 3). Chemerin is synthesized as a 162 amino acid (aa) precursor that contains a hydrophobic N-terminal sequence, an intervening 140 aa cystatin-fold containing domain, and a six aa C-terminal prosegment (4-6). Within the cystatin-fold domain there are three intrachain disulfide bonds that contribute to the characteristic fold (4, 7). The precursor molecule is described as undergoing proteolytic processing at both termini by unknown proteases. The N-terminal 16 residue hydrophobic segment is described as being either a signal sequence or a transmembrane (TM) segment for a type II TM protein (5, 8). In either case it gives rise to a soluble proform that undergoes further processing at the C‑terminus (5). In mouse, the C-terminal six residues are cleaved, giving rise to a monomeric, 16 kDa heparin-binding bioactive molecule (aa 17-156) (5-7). A shorter form has been described in human (7). The activity seems to be concentrated in the nine aa’s preceding the prosegment (aa 148-156). Retention of the prosegment blocks activity (4). The 140 aa mature segment is known to bind to the G-protein coupled receptor termed ChemR23 (5, 7). Binding results in macrophage and immature dendritic cell chemotaxis (5). The distribution of this receptor is limited to immune APCs, and it is assumed that Chemerin is an inflammatory molecule. It is unclear which cells are actually producing Chemerin, but keratinocytes, endothelial cells and osteoclasts are potential candidates (1, 7). Mature mouse Chemerin shares 67%, 84%, and 82% aa sequence identity to human, rat, and hamster Chemerin, respectively (6). There is apparently cross-species activity for the protein (6).

  1. Nagpal, S. et al. (1997) J. Invest. Dermatol. 109:91.
  2. Storici, P. et al. (1996) Eur. J. Biochem. 238:769.
  3. Zanetti, M. (2004) J. Leukoc. Biol. 75:39.
  4. Wittamer, V. et al. (2004) J. Biol. Chem. 279:9956.
  5. Wittamer, V. et al. (2003) J. Exp. Med. 198:977.
  6. Busmann, A. et al. (2004) J. Chromatog. B 811:217.
  7. Meder, W. et al. (2003) FEBS Lett. 555:495.
  8. Yokoyama-Kobayashi, M. et al. (1999) Gene 228:161.
Long Name
Retinoic Acid Receptor Responder Protein 2
Entrez Gene IDs
5919 (Human); 71660 (Mouse)
Alternate Names
Chemerin; RARRES2; RAR-responsive protein TIG2; retinoic acid receptor responder (tazarotene induced) 2; retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; TIG-2; TIG2HP10433

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Product Specific Notices

This product and/or its use is the subject of European Patent 1 405 083 B1, US Patents 7,332,291; 7,419,658 and 7,842,453 as well as foreign equivalents licensed to R&D Systems Inc. The purchase of this product is intended for research purposes only, not including the screening of compounds for the development of therapeutic and/or diagnostic products. Buyers may require a separate license to the patent rights for applications beyond such research purposes. For information on licensing please contact Euroscreen SA rue Adrienne Bolland n°47 B-6041 Gosselies Belgium. Phone: +32-71-348500, Fax: +32-71-348519, e-mail Attention: Dr. Vincent Lannoy.

Citations for Mouse Chemerin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Discovery of chemerin as the new chemoattractant of human mesenchymal stem cells
    Authors: I Kim, H Park, I Hwang, D Moon, H Yun, EJ Lee, HS Kim
    Cell & bioscience, 2021;11(1):120.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Astrocytes influence medulloblastoma phenotypes and CD133 surface expression
    Authors: E Gronseth, A Gupta, C Koceja, S Kumar, RG Kutty, K Rarick, L Wang, R Ramchandra
    PLoS ONE, 2020;15(7):e0235852.
    Species: Zebrafish
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Pro- and Anti-Inflammatory Role of ChemR23 Signaling in Pollutant-Induced Inflammatory Lung Responses.
    Authors: Provoost S, De Grove K, Fraser G, Lannoy V, Tournoy K, Brusselle G, Maes T, Joos G
    J Immunol, 2016;196(4):1882-90.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia
    Nat Commun, 2016;7(0):12528.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. The expression and regulation of chemerin in the epidermis.
    Authors: Banas M, Zegar A, Kwitniewski M, Zabieglo K, Marczynska J, Kapinska-Mrowiecka M, LaJevic M, Zabel B, Cichy J
    PLoS ONE, 2015;10(2):e0117830.
  6. Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis.
    Authors: Muruganandan S, Dranse H, Rourke J, McMullen N, Sinal C
    Stem Cells, 2013;31(10):2172-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. The Role of ChemR23 in the Induction and Resolution of Cigarette Smoke-Induced Inflammation.
    Authors: Demoor T, Bracke KR, Dupont LL
    J. Immunol., 2011;186(9):5457-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.
    Authors: Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ
    J. Biol. Chem., 2011;286(27):23982-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Chemerin peptides promote phagocytosis in a ChemR23- and Syk-dependent manner.
    Authors: Cash JL, Christian AR, Greaves DR
    J. Immunol., 2010;184(9):5315-24.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. Mouse ChemR23 is expressed in dendritic cell subsets and macrophages, and mediates an anti-inflammatory activity of chemerin in a lung disease model.
    Authors: Luangsay S, Wittamer V, Bondue B, De Henau O, Rouger L, Brait M, Franssen JD, de Nadai P, Huaux F, Parmentier M
    J. Immunol., 2009;183(10):6489-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC


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