Mouse Complement Component C1ra Antibody

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Detection of Mouse Complement Component C1ra by Western Blot.
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Product Details
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Mouse Complement Component C1ra Antibody Summary

Species Reactivity
Detects mouse Complement Component C1ra in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human C1r is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Complement Component C1ra
Accession # AAH04637
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Complement Component C1ra antibody by Western Blot. View Larger

Detection of Mouse Complement Component C1ra by Western Blot. Western blot shows mouse serum. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Component C1ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Complement Component C1ra at approximately 95, 60 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C1ra

C1rA (complement component 1 subcomponent r-A) is an 85-95 kDa member of the peptidase S1 family of enzymes. It is secreted primarily hepatocytes but is also known to be expressed by monocytes, fibroblasts, keratinocytes and endothelial cells. C1r forms a key component of the complement 1 complex. Complement is a general name for a group of molecules that "complement" the antimicrobial effort associated with antibodies and phagocytic cells. The C1 complex is composed of five proteins; one C1q that binds immunoglobulin, and two copies of two inactive serine proteases termed C1r and C1s. Activation of the C1 complex is the first step in a proteolytic cascade that generates multiple modulators involved in cell lysis and phagocytosis. Following C1q binding, each 85-90 kDa C1r monomer undergoes autocleavage to generate a 60 kDa and 28-34 kDa disulfide‑linked enzymatically-active heterodimer. Each C1r heterodimer now acts on a C1s counterpart to create a globally-active complex that drives the complement cascade. In contrast to human, the mouse genes for C1r and C1s have undergone duplication. The major, or liver derived, C1r is termed C1rA, while a prostate-associated C1r is called C1rB. Mouse C1rA proenzyme is 691 amino acids (aa) in length (aa 17-707) (SwissProt #: Q8CG16). It contains one CUB domain (aa 17-140), an EGF-like motif (aa 141-189), a second CUB domain (aa 192-303), two SUSHI (or CCP) repeats (aa 306-448), and a C-terminal peptidase S1 region (aa 463-704). Autocleavage occurs between Arg462:Ile463, generating a 60 kDa N-terminal subunit that is disulfide‑linked to a 30 kDa enzymatic subunit. Over aa 17-707, mouse C1rA shares 91% and 82% aa sequence identity with rat and human C1r, respectively. Full-length mouse C1rA shares 96% aa sequence identity with mouse C1rB, with virtually all differences arising in the enzymatic, or small subunit.

Entrez Gene IDs
50909 (Mouse)
Alternate Names
C1r;Complement C1r-A subcomponent;mC1;mC1rA;Peptidase S1 domain-containing protein; C1ra; Complement Component C1ra

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