Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Goat Anti-Mouse CX3CR1 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A) and Anti-Mouse Integrin alpha X/CD11c PE-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108A). View our protocol for Staining Membrane-associated Proteins.
Detection of CX3CR1 in CHO Chinese Hamster Cell Line Transfected with Mouse CX3CR1 and eGFP by Flow Cytometry.
CHO Chinese hamster ovary cell line transfected with either (A) mouse CX3CR1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse CX3CR1 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CX3CR1 (CX3C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.
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