Mouse CX3CR1 APC‑conjugated Antibody

Name: Mouse CX3CR1 APC‑conjugated Antibody
Brand: R&D Systems
SKU: FAB5825A
Price: 469 USD
Availability: InStock

R&D Systems | Catalog # FAB5825A

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG

View all CX3CR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9

Specificity

Detects mouse CX3CR1 in direct ELISAs. Stains mouse CX3CR1 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CX3CR1 APC‑conjugated Antibody

Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A) and Anti-Mouse Integrin aX/CD11c PE-conjugated Monoclonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108A). View our protocol for Staining Membrane-associated Proteins.

Detection of CX3CR1 in CHO Chinese Hamster Cell Line Transfected with Mouse CX3CR1 and eGFP by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with either (A) mouse CX3CR1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse CX3CR1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825A). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CX3CR1 APC‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes and HEK293 cell line transfected with eGFP and mouse CX3CR1

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CR1

CX3CR1 (CX₃C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa member of the delta -group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1^lo monocytes plus smooth muscle and mast cells. CX₃CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX₃CR1 shares 96% and 77% aa identity with rat and human CX₃CR1, respectively.