Mouse CX3CR1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (4)
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Mouse CX3CR1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Detects human and mouse CX3CR1 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)


Recommended Concentration
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CX3CR1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Hamster Anti-Mouse CD11c PE-conjugated Monoclonal Antibody (Catalog # FAB69501P) and either (A) Goat Anti-Mouse CX3CR1 Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825G) or (B) Normal Goat IgG Alexa Fluor 488 Control (Catalog # IC108G). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CR1

CX3CR1 (CX3C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.

Long Name
CX3C Chemokine Receptor 1
Entrez Gene IDs
1524 (Human); 13051 (Mouse); 171056 (Rat)
Alternate Names
Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3CR1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Mouse CX3CR1 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage differentiation in the colon
    Authors: A Nakamura, S Kurihara, D Takahashi, W Ohashi, Y Nakamura, S Kimura, M Onuki, A Kume, Y Sasazawa, Y Furusawa, Y Obata, S Fukuda, S Saiki, M Matsumoto, K Hase
    Nature Communications, 2021-04-08;12(1):2105.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. SOCS3 deficiency in myeloid cells promotes retinal degeneration and angiogenesis through arginase-1 up-regulation in experimental autoimmune uveoretinitis
    Authors: M Chen, J Zhao, IH Ali, S Marry, J Augustine, M Bhuckory, A Lynch, A Kissenpfen, H Xu
    Am. J. Pathol., 2018-02-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Comprehensive overview of murine epididymal mononuclear phagocytes and lymphocytes: Unexpected populations arise
    Authors: A Voisin, M Whitfield, C Damon-Soub, C Goubely, J Henry-Berg, F Saez, A Kocer, JR Drevet, R Guiton
    J. Reprod. Immunol., 2018-01-31;126(0):11-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Atorvastatin improves plaque stability in ApoE-knockout mice by regulating chemokines and chemokine receptors.
    Authors: Nie P, Li D, Hu L, Jin S, Yu Y, Cai Z, Shao Q, Shen J, Yi J, Xiao H, Shen L, He B
    PLoS ONE, 2014-05-09;9(5):e97009.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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