Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG
View all CX3CR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9

Specificity

Detects mouse CX3CR1 in direct ELISAs.  Stains mouse CX3CR1 transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CX3CR1 PE‑conjugated Antibody

Detection of CX3CR1 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse CX3CR1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825P) and Anti-Mouse Integrin aX/CD11c FITC-conjugated Mono-clonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Detection of CX3CR1 antibody in CHO Chinese Hamster Cell Line Transfected with Mouse CX3CR1 and eGFP antibody by Flow Cytometry.

Detection of CX3CR1 in CHO Chinese Hamster Cell Line Transfected with Mouse CX3CR1 and eGFP by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with either (A) mouse CX3CR1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse CX3CR1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825P). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CX3CR1 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CHO Chinese hamster ovary cell line transfected with mouse CX3CR1 and eGFP

Reviewed Applications

Read 2 reviews rated 4.5 using FAB5825P in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CX3CR1

CX3CR1 (CX3C chemokine receptor 1; also known as fractalkine receptor, GPCR13 and V28) is a 40 kDa member of the delta -group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.

Long Name

CX3C Chemokine Receptor 1

Alternate Names

CMKbRL1, V28

Entrez Gene IDs

1524 (Human); 13051 (Mouse); 171056 (Rat)

Gene Symbol

CX3CR1

UniProt

Q9Z0D9

Additional CX3CR1 Products

Product Documents for Mouse CX3CR1 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CX3CR1 PE‑conjugated Antibody

For research use only

Citations for Mouse CX3CR1 PE‑conjugated Antibody

Customer Reviews for Mouse CX3CR1 PE‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Human/Mouse CX3CR1 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 04/04/2019
    Viable CNS mononuclear cells isolated at symptom onset of EAE and stained for CX3CR1 (blue) and FMO for CX3CR1 (red).
    Experimental autoimmune encephalomyelitis was induced in C57BL6/J mice, and mononuclear cells were isolated from the CNS when the animals were at the onset of symptoms (day 10). Cells were stained for CX3CR1, CCR2, CD45, CD11b, CD11c, CD4, CD8, CCR2, CCR5, CCR7, and CXCR4, plus for viability to exclude dead cells. Viable cells stained for CX3CR1 (blue) and FMO sample for CX3CR1 (stained for all the markers except for CX3CR1; red).
    Mouse CX3CR1 PE‑conjugated Antibody FAB5825P
  • Mouse CX3CR1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Adult brain
    Species: Mouse
    Verified Customer | Posted 08/18/2017
    Mouse CX3CR1 PE‑conjugated Antibody FAB5825P

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