Mouse CX3CR1 PE-conjugated Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CX3CR1 in direct ELISAs.  Stains mouse CX3CR1 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CX3CR1
    Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
    Accession # Q9Z0D9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CX3CR1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CX3CR1 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825P) and Anti-Mouse Integrin alpha X/CD11c FITC-conjugated Mono­clonal Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Detection of CX3CR1 in CHO Chinese Hamster Cell Line Transfected with Mouse CX3CR1 and eGFP by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with either
(A) mouse CX3CR1 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Mouse CX3CR1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB5825P). Quadrant markers were set based on control antibody staining (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CX3CR1
CX3CR1 (CX3C chemokine receptor 1; also known as fractalkine receptor, GPCR13 and V28) is a 40 kDa member of the δ-group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.
  • Long Name:
    CX3C Chemokine Receptor 1
  • Entrez Gene IDs:
    1524 (Human); 13051 (Mouse); 171056 (Rat)
  • Alternate Names:
    Beta chemokine receptor-like 1; CCRL1; chemokine (C-C) receptor-like 1; chemokine (C-X3-C motif) receptor 1; chemokine (C-X3-C) receptor 1; CMKbRL1; CMK-BRL1; CMK-BRL-1; CMKDR1; CX3C chemokine receptor 1; CX3CR1; Fractalkine receptor; G protein-coupled receptor 13; GPR13G-protein coupled receptor 13; GPRV28; V28; V28C-X3-C CKR-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Gut microbiota influences pathological angiogenesis in obesity-driven choroidal neovascularization
    EMBO Mol Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Monocyte-Derived Macrophages Contribute to Spontaneous Long-Term Functional Recovery after Stroke in Mice
    Authors: S Wattananit, D Tornero, N Graubardt, T Memanishvi, E Monni, J Tatarishvi, G Miskinyte, R Ge, H Ahlenius, O Lindvall, M Schwartz, Z Kokaia
    J Neurosci, 2016;36(15):4182-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Transgenic expression of GM-CSF in T cells causes disseminated histiocytosis.
    Authors: van Nieuwenhuijze A, Coghill E, Gray D, Prato S, Metcalf D, Alexander W, Wicks I
    Am J Pathol, 2014;184(1):184-99.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 8  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Mouse CX3CR1 PE-conjugated Antibody FAB5825P
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 Flow Mouse Anonymous 08/18/2017
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Flow Cytometry Mouse CX3CR1 PE-conjugated Antibody FAB5825P
Flow Cytometry: Mouse CX3CR1 PE-conjugated Antibody [FAB5825P]

Summary

ApplicationFlow Cytometry
Sample TestedAdult brain
SpeciesMouse

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