CX3CR1 Antibody

R&D Systems | Catalog # AF5825

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CX3CR1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CX3CR1
Met1-Thr32, Leu92-Lys104, Thr169-Val196, Lys258-Leu274
Accession # Q9Z0D9

Specificity

Detects human and mouse CX3CR1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CX3CR1 Antibody

Detection of Human and Mouse CX3CR1 antibody by Western Blot.

Detection of Human and Mouse CX3CR1 by Western Blot.

Western blot shows lysates of M1 mouse myeloid leukemia cell line and NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CX3CR1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CX3CR1 antibody in M1 Mouse Cell Line antibody by Flow Cytometry.

Detection of CX3CR1 in M1 Mouse Cell Line by Flow Cytometry.

M1 mouse myeloid leukemia cell line was stained with Human/Mouse CX3CR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5825, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for CX3CR1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: M1 mouse myeloid leukemia cell line

Western Blot

1 µg/mL
Sample: M1 mouse myeloid leukemia cell line and NK‑92 human natural killer lymphoma cell line

Reviewed Applications

Read 2 reviews rated 4.5 using AF5825 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CR1

CX3CR1 (CX3C chemokine receptor 1; also fractalkine receptor, GPCR13 and V28) is a 40 kDa (predicted) member of the delta -group of rhodopsin GPCRs. It is expressed on astrocytes, microglia, macrophages, Th1 and Tc1 T cells, NK cells, mouse Gr1lo monocytes plus smooth muscle and mast cells. CX3CR1 mediates adhesion to fractalkine, promotes avid binding of integrins to their ligands, and extends the life of monocytes. Mouse CX3CR1 is a 7-transmembrane protein that is 354 amino acids (aa) in length. It contains a 32 aa N-terminal extracellular region that shows no glycosylation, and a 56 aa C-terminal cytoplasmic domain. Over aa 1‑32, 91‑104, 169‑196 and 258‑274 collectively, mouse CX3CR1 shares 96% and 77% aa identity with rat and human CX3CR1, respectively.

Long Name

CX3C Chemokine Receptor 1

Alternate Names

CMKbRL1, V28

Entrez Gene IDs

1524 (Human); 13051 (Mouse); 171056 (Rat)

Gene Symbol

CX3CR1

UniProt

Q9Z0D9

Additional CX3CR1 Products

Product Documents for CX3CR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for CX3CR1 Antibody

For research use only

Citations for CX3CR1 Antibody

Customer Reviews for CX3CR1 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Human/Mouse CX3CR1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HMC3 (human microglia cell line) lysate
    Species: Human
    Verified Customer | Posted 09/11/2019
    CX3CR1 Antibody AF5825
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21888931
    Species: Human
    Verified Customer | Posted 01/08/2015

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