KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulants for CFU-GM. As a chemoattractant for hCXCR2 transfected mouse Baf/3 cells, the truncated form of mouse KC (aa 29-96) is also approximately 5-fold more active than 77 aa residue form of KC (aa 20-96, R&D Systems, Catalog # 453-KC) (1-4).
Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody
R&D Systems | Catalog # BAF453
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
Flow Cytometry, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse CXCL1/KC
Arg20-Lys96
Accession # P12850
Arg20-Lys96
Accession # P12850
Specificity
Detects mouse CXCL1/GRO alpha /KC/CINC-1 in ELISAs and Western blots. In sandwich immunoassays, less than 40% cross-reactivity with recombinant rat (rr) CINC-1 is observed and less than 0.05% cross-reactivity with rrCINC-2 alpha, rrCINC-2 beta, recombinant mouse (rm) CRG-2, rmMIG, and rmMIP-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)
Sample: Recombinant Mouse CXCL1/GRO alpha /KC/CINC‑1 aa 20-96 (Catalog # 453-KC)
Mouse CXCL1/KC Sandwich Immunoassay
ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
Use in combination with these reagents:
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using BAF453 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CXCL1/GRO alpha/KC/CINC-1
References
- King, A.G. et al. (2000) J. Immunol. 164:3774.
- Oquendo, P. et al. (1989) J. Biol. Chem. 264:4133.
- Huo, Y. et al. (2001) J. Clin. Invest. 108:1307.
- Xia, M. et al. (2002) J. Neuroimmunol. 122:55.
Alternate Names
CINC-1, CINC1, GRO alpha, KC, MGSA-alpha
Gene Symbol
CXCL1
UniProt
Additional CXCL1/GRO alpha/KC/CINC-1 Products
Product Documents for Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody
For research use only
Related Research Areas
Citations for Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody
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Customer Reviews for Mouse CXCL1/GRO alpha /KC/CINC‑1 Biotinylated Antibody (2)
5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: SerumSpecies: MouseVerified Customer | Posted 11/29/2022Used as ELISA detection
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Application: ELISASample Tested: Serum and PlasmaSpecies: MouseVerified Customer | Posted 01/05/2018This antibody was used as a matched pair with MAB453 in an ELISA measuring KC in mouse samples.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
