Mouse CXCL10/IP-10/CRG-2 Antibody MAB466-100: R&D Systems

Mouse CXCL10/IP-10/CRG-2 Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) CXCL1, 2, 3, 5, 6, 7, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, rmCXCL1, 2, 6, 9, 11, 12/SDF-1 alpha, 13, rpCXCL8, rrCXCL1, 3/CINC-2 alpha, 3/CINC-2 beta
  • Source
    Monoclonal Rat IgG2A Clone # 134013
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2
    Ile22-Pro98
    Accession # P17515
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CXCL10/IP‑10/CRG‑2 (Catalog # 466-CR)
    • Mouse CXCL10/IP-10/CRG-2 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CXCL10/IP‑10/CRG‑2 Antibody (Catalog # MAB466)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog # BAF466)
  • ELISA Standard
     
    Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (Catalog # 466-CR)
  • Neutralization
    Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 10-40 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CXCL10/IP‑10/CRG‑2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL10/CRG‑2 and Neutralization by Mouse CXCL10/CRG‑2 Antibody. Recombinant Mouse CXCL10/CRG‑2 (Catalog # 466-CR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/CRG‑2 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL10/CRG‑2 Monoclonal Antibody (Catalog # MAB466). The ND50 is typically 10-40 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL10/IP-10/CRG-2

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

  • References:
    1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
    2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
    3. Sgadari, C. et al. (1996) Blood, 87:3877.
  • Entrez Gene IDs:
    3627 (Human); 15945 (Mouse)
  • Alternate Names:
    C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
  2. Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy.
    Authors: Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M
    Nat Immunol, 2015;16(8):850-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease.
    Authors: Ioannidis L, Nie C, Ly A, Ryg-Cornejo V, Chiu C, Hansen D
    J Immunol, 2015;196(3):1227-38.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Capture
  4. Tumor-infiltrating myeloid-derived suppressor cells are pleiotropic-inflamed monocytes/macrophages that bear M1- and M2-type characteristics.
    Authors: Umemura N, Saio M, Suwa T, Kitoh Y, Bai J, Nonaka K, Ouyang GF, Okada M, Balazs M, Adany R, Shibata T, Takami T
    J. Leukoc. Biol., 2008;83(5):1136-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Secondary Antibodies
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Goat Anti-Rat IgG Biotinylated Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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