Mouse CXCL10/IP-10/CRG-2 Antibody

Catalog # Availability Size / Price Qty
MAB466-SP
MAB466-500
MAB466-100
Chemotaxis Induced by CXCL10/CRG‑2 and Neutralization by Mouse CXCL10/CRG‑2 Antibody.
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Product Details
Citations (21)
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Supplemental Products
Reviews (1)

Mouse CXCL10/IP-10/CRG-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) CXCL1, 2, 3, 5, 6, 7, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, rmCXCL1, 2, 6, 9, 11, 12/SDF-1 alpha, 13, rpCXCL8, rrCXCL1, 3/CINC-2 alpha, 3/CINC-2 beta
Source
Monoclonal Rat IgG2A Clone # 134013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2
Ile22-Pro98
Accession # P17515
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CXCL10/IP‑10/CRG‑2 (Catalog # 466-CR)

Mouse CXCL10/IP-10/CRG-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog # BAF466)

Standard: Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (Catalog # 466-CR)

Neutralization
Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 10-40 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CXCL10/IP‑10/CRG‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL10/CRG‑2 and Neutralization by Mouse CXCL10/CRG‑2 Antibody. View Larger

Chemotaxis Induced by CXCL10/CRG‑2 and Neutralization by Mouse CXCL10/CRG‑2 Antibody. Recombinant Mouse CXCL10/CRG‑2 (Catalog # 466-CR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/CRG‑2 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL10/CRG‑2 Monoclonal Antibody (Catalog # MAB466). The ND50 is typically 10-40 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL10/IP-10/CRG-2

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
  3. Sgadari, C. et al. (1996) Blood, 87:3877.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

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Citations for Mouse CXCL10/IP-10/CRG-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. MLKL signaling regulates macrophage polarization in acute pancreatitis through CXCL10
    Authors: C Peng, G Tu, J Wang, Y Wang, P Wu, L Yu, Z Li, X Yu
    Cell Death & Disease, 2023-02-24;14(2):155.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Lacticaseibacillus paracasei sh2020 induced antitumor immunity and synergized with anti-programmed cell death 1 to reduce tumor burden in mice
    Authors: Shi-Long Zhang, Bing Han, Yu-Qin Mao, Zheng-Yan Zhang, Zhan-Ming Li, Chao-Yue Kong et al.
    Gut Microbes
  3. MYC inhibition reprograms tumor immune microenvironment by recruiting T lymphocytes and activating the CD40/CD40L system in osteosarcoma
    Authors: Kuo Jiang, Qianfeng Zhang, Yong Fan, Jia Li, Jitao Zhang, Wentao Wang et al.
    Cell Death Discovery
  4. The selective STING inhibitor H-151 preserves myocardial function and ameliorates cardiac fibrosis in murine myocardial infarction
    Authors: S Hu, Y Gao, R Gao, Y Wang, Y Qu, J Yang, X Wei, F Zhang, J Ge
    International immunopharmacology, 2022-03-09;107(0):108658.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  5. Chronic kidney disease mediates cardiac dysfunction associated with increased resident cardiac macrophages
    Authors: MA Mawhin, RG Bright, JD Fourre, EI Vloumidi, J Tomlinson, A Sardini, CD Pusey, KJ Woollard
    Bmc Nephrology, 2022-01-28;23(1):47.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis
    Authors: MH Mursalin, PS Coburn, FC Miller, ET Livingston, R Astley, MC Callegan
    Investigative Ophthalmology & Visual Science, 2021-11-01;62(14):14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. CXCL10/CXCR3 signaling contributes to an inflammatory microenvironment and its blockade enhances progression of murine pancreatic precancerous lesions
    Authors: Veethika Pandey, Alicia Fleming-Martinez, Ligia Bastea, Heike R Doeppler, Jillian Eisenhauer, Tam Le et al.
    eLife
  8. circFAT1 Promotes Cancer Stemness and Immune Evasion by Promoting STAT3 Activation
    Authors: Lingfei Jia, Yilun Wang, Cun‐Yu Wang
    Advanced Science
  9. Cytotoxic T cells swarm by homotypic chemokine signalling
    Authors: Jorge Luis Galeano Niño, Sophie V Pageon, Szun S Tay, Feyza Colakoglu, Daryan Kempe, Jack Hywood et al.
    eLife
  10. CDK4/6 inhibition promotes immune infiltration in ovarian cancer and synergizes with PD-1 blockade in a B cell-dependent manner
    Authors: QF Zhang, J Li, K Jiang, R Wang, JL Ge, H Yang, SJ Liu, LT Jia, L Wang, BL Chen
    Theranostics, 2020-08-25;10(23):10619-10633.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Migration Assay
  11. Early growth response 1 deficiency protects host against Pseudomonas aeruginosa lung infection
    Authors: Z Pang, R Raudonis, C McCormick, Z Cheng
    Infect. Immun., 2019-12-17;0(0):.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Capture
  12. Translational repression of Ccl5 and Cxcl10 by 4E-BP1 and 4E-BP2 restrains the ability of mouse macrophages to induce migration of activated T�cells
    Authors: M William, LP Leroux, V Chaparro, TE Graber, T Alain, M Jaramillo
    Eur. J. Immunol., 2019-05-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Nitrosporeusine analogue ameliorates Chandipura virus induced inflammatory response in CNS via NF?b inactivation in microglia
    Authors: AK Verma, TS Waghmare, GR Jachak, SC Philkhana, DS Reddy, A Basu
    PLoS Negl Trop Dis, 2018-07-12;12(7):e0006648.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  14. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  15. Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease.
    Authors: Ioannidis L, Nie C, Ly A, Ryg-Cornejo V, Chiu C, Hansen D
    J Immunol, 2015-12-30;196(3):1227-38.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  16. Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy.
    Authors: Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M
    Nat Immunol, 2015-06-15;16(8):850-8.
    Species: Mouse
    Sample Types: In Vivo, Plasma
    Applications: ELISA Development (Capture), Neutralization
  17. The Early Activation Marker CD69 Regulates the Expression of Chemokines and CD4 T Cell Accumulation in Intestine
    Authors: Katarina Radulovic, Valerio Rossini, Calin Manta, Karlheinz Holzmann, Hans A. Kestler, Jan Hendrik Niess
    PLoS ONE
  18. Atherosclerotic Plaque Stability Is Affected by the Chemokine CXCL10 in Both Mice and Humans
    Authors: Dolf Segers, Jonathan A. Lipton, Pieter J. M. Leenen, Caroline Cheng, Dennie Tempel, Gerard Pasterkamp et al.
    International Journal of Inflammation
  19. T Cell-, Interleukin-12-, and Gamma Interferon-Driven Viral Clearance in Measles Virus-Infected Brain Tissue
    Authors: Samantha R. Stubblefield Park, Mi Widness, Alan D. Levine, Catherine E. Patterson
    Journal of Virology
  20. Tumor-infiltrating myeloid-derived suppressor cells are pleiotropic-inflamed monocytes/macrophages that bear M1- and M2-type characteristics.
    Authors: Umemura N, Saio M, Suwa T, Kitoh Y, Bai J, Nonaka K, Ouyang GF, Okada M, Balazs M, Adany R, Shibata T, Takami T
    J. Leukoc. Biol., 2008-02-19;83(5):1136-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. CXC Motif Chemokine 10 Impairs Autophagy and Autolysosome Formation in Non-alcoholic Steatohepatitis
    Authors: Zhang X, Wu WK, Xu W et al.
    Theranostics. 

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Mouse CXCL10/IP-10/CRG-2 Antibody
By Anonymous on 09/06/2021
Application: WB Sample Tested: BaF3 mouse pro‑B cell line Species: Mouse