CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.
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Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
R&D Systems | Catalog # DY466
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Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
62.5-4000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse
Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
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Product Summary for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CXCL10/IP-10/CRG-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
Mouse CXCL10 / IP-10 / CRG-2 ELISA Standard Curve
Kit Contents for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)
Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)
Microplates: (Catalog # DY990), or equivalent
Plate Sealers: (Catalog # DY992), or equivalent
*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL10/IP-10/CRG-2
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
For research use only
Citations for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
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Customer Reviews for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA (10)
4.7 out of 5
10 Customer Ratings
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Sample Tested: mouse serumSpecies: MouseVerified Customer | Posted 03/26/2026The DuoSet ELISA CXCL10 kit was used to quantify CXCL10 levels in mouse serum samples. Blood was collected from mice and allowed to clot before centrifugation to obtain serum. Samples were stored at -80°C until analysis. Prior to the assay, serum samples were thawed and diluted appropriately to fall within the dynamic range of the standard curve. The ELISA was performed according to the manufacturer’s instructions, including plate coating, blocking, incubation with standards and samples, and detection using the provided antibody system. All samples and standards were run in duplicates, and absorbance was measured using a microplate reader at the recommended wavelength. The assay enabled reliable quantification of CXCL10 levels across the experimental groups.
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Sample Tested: Cell culture supernatantVerified Customer | Posted 03/11/2022
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Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line and Cell culture supernatantVerified Customer | Posted 11/10/2019
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Sample Tested: MOUSE MACROPHAGEVerified Customer | Posted 09/11/2019
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Sample Tested: Serum and PlasmaVerified Customer | Posted 09/07/2019
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Sample Tested: Bone marrow cellsVerified Customer | Posted 07/04/2019Mouse Bone Marrow Derived Macrophages
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Sample Tested: Embryonic cortical glial cellsVerified Customer | Posted 06/20/2019
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Sample Tested: Bone marrow derived macrophages- supernatants and Cell culture supernatantVerified Customer | Posted 10/16/2016The protocol was clear to follow and all the reagents were marked, but I wish the resuspension volumes were on the protocol sheet and not the Certificate of Analysis.
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Sample Tested: Cell culture supernatantVerified Customer | Posted 10/06/2016
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Sample Tested: Purified murine IP-10 proteinVerified Customer | Posted 10/26/201550ul of murine IL-10 Capture antibody (2ug/mL) was coated per well of a 96 well plate at 4 degrees overnight. Purified mIP-10 standard was diluted (7 fold) and assayed to ensure the quality of the ELISA. Mouse IP-10 Detector antibody was added (2h @ RT) followed by a 20 min incubation with Streptavidin-HRP. Substrate solution was added to measure mIP-10 concentration according to the manufactures protocol. The standard curve looked great as can be seen by calculation of the R- squared value. Highly recommend doing ELISA's using kits from R&D. <br />Buffer: 7-point standard curve
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Protocols
View specific protocols for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA (DY466):
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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