Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY466
DY466-05
Ancillary Products Available
CXCL10/IP-10/CRG-2 ELISA Kit ELISA
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Product Details
Procedure
Citations (43)
FAQs
Supplemental Products
Reviews (3)

Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CXCL10/IP-10/CRG-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Mouse CXCL10 / IP-10 / CRG-2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL10/IP-10/CRG-2

IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.

Entrez Gene IDs:
3627 (Human); 15945 (Mouse)
Alternate Names:
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines
    Authors: PLD Silva, F Lauretti-F, M Caldas de, SS Lima, AE Covarrubia, M De Franco, E Carvalho, PL Ho, RMA da Costa, EAL Martins, JB Da Silva
    BMC Microbiol., 2019;19(1):4.
    Species: Mouse
    Sample Types: Cell Culture Supernate
  2. Hu Antigen R Regulates Antiviral Innate Immune Responses through the Stabilization of mRNA for Polo-like Kinase 2
    Authors: T Sueyoshi, T Kawasaki, Y Kitai, D Ori, S Akira, T Kawai
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. DNA-PK Is Targeted by Multiple Vaccinia Virus Proteins to Inhibit DNA Sensing
    Authors: SR Scutts, SW Ember, H Ren, C Ye, CA Lovejoy, M Mazzon, DL Veyer, RP Sumner, GL Smith
    Cell Rep, 2018;25(7):1953-1965.e4.
    Species: Mouse
    Sample Types: Cell Culture Supernate
  4. Microphthalmia-Associated Transcription Factor (MITF) Regulates Immune Cell Migration into Melanoma
    Authors: GM Wiedemann, C Aithal, A Kraechan, C Heise, BL Cadilha, J Zhang, P Duewell, R Ballotti, S Endres, C Bertolotto, S Kobold
    Transl Oncol, 2018;12(2):350-360.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Lipoteichoic acid anchor triggers Mincle to drive protective immunity against invasive group A Streptococcus infection
    Authors: T Imai, T Matsumura, S Mayer-Lamb, CA Wells, E Ishikawa, SK Butcher, TC Barnett, MJ Walker, A Imamura, H Ishida, T Ikebe, T Miyamoto, M Ato, S Ohga, B Lepenies, NM van Sorge, S Yamasaki
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
  6. Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations
    Authors: A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston
    Nat Commun, 2018;9(1):2229.
    Species: Mouse
    Sample Types: BALF
  7. Interspecies cathelicidin comparison reveals divergence in antimicrobial activity, TLR modulation, chemokine induction and regulation of phagocytosis
    Authors: M Coorens, MR Scheenstra, EJ Veldhuizen, HP Haagsman
    Sci Rep, 2017;7(0):40874.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. SSeCKS/AKAP12 scaffolding functions suppress B16F10-induced peritoneal metastasis by attenuating CXCL9/10 secretion by resident fibroblasts
    Authors: M Muramatsu, L Gao, J Peresie, B Balderman, S Akakura, IH Gelman
    Oncotarget, 2017;8(41):70281-70298.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. NF-?B signaling regulates cell-autonomous regulation of CXCL10 in breast cancer 4T1 cells
    Authors: WJ Jin, B Kim, D Kim, HY Park Choo, HH Kim, H Ha, ZH Lee
    Exp. Mol. Med, 2017;49(2):e295.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. TLR7 and TLR3 Sense Brucella abortus RNA to Induce Proinflammatory Cytokine Production but They Are Dispensable for Host Control of Infection
    Authors: PC Campos, MT Gomes, ES Guimarães, G Guimarães, SC Oliveira
    Front Immunol, 2017;8(0):28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Cathelicidins Inhibit Escherichia coli-Induced TLR2 and TLR4 Activation in a Viability-Dependent Manner
    Authors: M Coorens, VAF Schneider, AM de Groot, A van Dijk, M Meijerink, JM Wells, MR Scheenstra, EJA Veldhuizen, HP Haagsman
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Effects of fumarates on inflammatory human astrocyte responses and oligodendrocyte differentiation
    Authors: DA Galloway, JB Williams, CS Moore
    Ann Clin Transl Neurol, 2017;4(6):381-391.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Repurposed JAK1/JAK2 Inhibitor Reverses Established Autoimmune Insulitis in Non-Obese Diabetic Mice
    Authors: PM Trivedi, KL Graham, NA Scott, MR Jenkins, S Majaw, RM Sutherland, S Fynch, AM Lew, CJ Burns, B Krishnamur, TC Brodnicki, SI Mannering, TW Kay, HE Thomas
    Diabetes, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production
    Authors: P Shinde, W Liu, A Ménoret, AD Luster, AT Vella
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  15. Allogeneic Compact Bone-Derived Mesenchymal Stem Cell Transplantation Attenuates the Severity of Idiopathic Pneumonia Syndrome in a Murine Bone Marrow Transplantation Model
    Authors: Shu-Kai Qiao
    Cell. Physiol. Biochem, 2016;40(6):1656-1669.
    Species: Mouse
    Sample Types: BALF
  16. The cardiac maladaptive ATF3-dependent cross-talk between cardiomyocytes and macrophages is mediated by the IFN?-CXCL10-CXCR3 axis
    Authors: A Aronheim
    Int. J. Cardiol., 2016;228(0):394-400.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Protease-Activated Receptor 1 Enhances Poly I:C Induction of the Antiviral Response in Macrophages and Mice
    Authors: Nigel Mackman
    J Innate Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. CXCR3 signaling in BRAFWT melanoma increases IL-8 expression and tumorigenicity.
    Authors: Jenkins, Molly H, Brinckerhoff, Constanc, Mullins, David W
    PLoS ONE, 2015;10(3):e0121140.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. IRGM3 contributes to immunopathology and is required for differentiation of antigen-specific effector CD8+ T cells in experimental cerebral malaria.
    Authors: Guo J, McQuillan J, Yau B, Tullo G, Long C, Bertolino P, Roediger B, Weninger W, Taylor G, Hunt N, Ball H, Mitchell A
    Infect Immun, 2015;83(4):1406-17.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Th1-Like ICOS+ Foxp3+ Treg Cells Preferentially Express CXCR3 and Home to beta-Islets during Pre-Diabetes in BDC2.5 NOD Mice.
    Authors: Kornete M, Mason E, Girouard J, Lafferty E, Qureshi S, Piccirillo C
    PLoS ONE, 2015;10(5):e0126311.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy.
    Authors: Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M
    Nat Immunol, 2015;16(8):850-8.
    Species: Mouse
    Sample Types: Plasma
  22. Human beta-D-3 Exacerbates MDA5 but Suppresses TLR3 Responses to the Viral Molecular Pattern Mimic Polyinosinic:Polycytidylic Acid.
    Authors: Semple F, MacPherson H, Webb S, Kilanowski F, Lettice L, McGlasson S, Wheeler A, Chen V, Millhauser G, Melrose L, Davidson D, Dorin J
    PLoS Genet, 2015;11(12):e1005673.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Negative regulation of melanoma differentiation-associated gene 5 (MDA5)-dependent antiviral innate immune responses by Arf-like protein 5B.
    Authors: Kitai Y, Takeuchi O, Kawasaki T, Ori D, Sueyoshi T, Murase M, Akira S, Kawai T
    J Biol Chem, 2015;290(2):1269-80.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. The transcription factor ATF7 mediates lipopolysaccharide-induced epigenetic changes in macrophages involved in innate immunological memory.
    Authors: Yoshida K, Maekawa T, Zhu Y, Renard-Guillet C, Chatton B, Inoue K, Uchiyama T, Ishibashi K, Yamada T, Ohno N, Shirahige K, Okada-Hatakeyama M, Ishii S
    Nat Immunol, 2015;16(10):1034-43.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Animal model of respiratory syncytial virus: CD8+ T cells cause a cytokine storm that is chemically tractable by sphingosine-1-phosphate 1 receptor agonist therapy.
    Authors: Walsh K, Teijaro J, Brock L, Fremgen D, Collins P, Rosen H, Oldstone M
    J Virol, 2014;88(11):6281-93.
    Species: Mouse
    Sample Types: BALF
  26. Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis
    Authors: L van Bon, AJ Affandi, J Broen, RB Christmann, RJ Marijnisse, L Stawski, GA Farina, G Stifano, AL Mathes, M Cossu, M York, C Collins, M Wenink, R Huijbens, R Hesselstra, T Saxne, M DiMarzio, D Wuttge, SK Agarwal, JD Reveille, S Assassi, M Mayes, Y Deng, JP Drenth, J de Graaf, M den Heijer, CG Kallenberg, M Bijl, A Loof, WB van den Be, LA Joosten, V Smith, F de Keyser, R Scorza, C Lunardi, PL van Riel, M Vonk, W van Heerde, S Meller, B Homey, L Beretta, M Roest, M Trojanowsk, R Lafyatis, TR Radstake
    N. Engl. J. Med, 2014;370(5):433-43.
    Species: Human
    Sample Types: Plasma
  27. Mapping the innate signaling cascade essential for cytokine storm during influenza virus infection.
    Authors: Teijaro J, Walsh K, Rice S, Rosen H, Oldstone M
    Proc Natl Acad Sci U S A, 2014;111(10):3799-804.
    Species: Mouse
    Sample Types: BALF
  28. Circadian clock function is disrupted by environmental tobacco/cigarette smoke, leading to lung inflammation and injury via a SIRT1-BMAL1 pathway.
    Authors: Hwang J, Sundar I, Yao H, Sellix M, Rahman I
    FASEB J, 2014;28(1):176-94.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. Toll-like receptor 9 mediated responses in cardiac fibroblasts.
    Authors: Ohm I, Alfsnes K, Belland Olsen M, Ranheim T, Sandanger O, Dahl T, Aukrust P, Finsen A, Yndestad A, Vinge L
    PLoS ONE, 2014;9(8):e104398.
  30. A pseudopterane diterpene isolated from the octocoral Pseudopterogorgia acerosa inhibits the inflammatory response mediated by TLR-ligands and TNF-alpha in macrophages.
    Authors: Gonzalez Y, Doens D, Santamaria R, Ramos M, Restrepo C, Barros de Arruda L, Lleonart R, Gutierrez M, Fernandez P
    PLoS ONE, 2013;8(12):e84107.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Oxidative damage of DNA confers resistance to cytosolic nuclease TREX1 degradation and potentiates STING-dependent immune sensing.
    Authors: Gehrke N, Mertens C, Zillinger T, Wenzel J, Bald T, Zahn S, Tuting T, Hartmann G, Barchet W
    Immunity, 2013;39(3):482-95.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  32. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
    Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
    PLoS ONE, 2012;7(2):e32125.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  33. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2012;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  34. Suppression of cytokine storm with a sphingosine analog provides protection against pathogenic influenza virus.
    Authors: Walsh KB, Teijaro JR, Wilker PR
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(29):12018-23.
    Species: Mouse
    Sample Types: BALF
  35. Phosphoinositide 3-kinase gamma plays a critical role in bleomycin-induced pulmonary inflammation and fibrosis in mice.
    Authors: Russo RC, Garcia CC, Barcelos LS, Rachid MA, Guabiraba R, Roffe E, Souza AL, Sousa LP, Mirolo M, Doni A, Cassali GD, Pinho V, Locati M, Teixeira MM
    J. Leukoc. Biol., 2011;89(2):269-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
  36. Endothelial cells are central orchestrators of cytokine amplification during influenza virus infection.
    Authors: Teijaro JR, Walsh KB, Cahalan S, Fremgen DM, Roberts E, Scott F, Martinborough E, Peach R, Oldstone MB, Rosen H
    Cell, 2011;146(6):980-91.
    Species: Mouse
    Sample Types: BALF
  37. Systemic inhibition of transforming growth factor-beta in glioma-bearing mice improves the therapeutic efficacy of glioma-associated antigen peptide vaccines.
    Authors: Ueda R, Fujita M, Zhu X, Sasaki K, Kastenhuber ER, Kohanbash G, McDonald HA, Harper J, Lonning S, Okada H
    Clin. Cancer Res., 2009;15(21):6551-9.
    Species: Mouse
    Sample Types: Plasma
  38. Scavenging roles of chemokine receptors: chemokine receptor deficiency is associated with increased levels of ligand in circulation and tissues.
    Authors: Cardona AE, Sasse ME, Liu L, Cardona SM, Mizutani M, Savarin C, Hu T, Ransohoff RM
    Blood, 2008;112(2):256-63.
    Species: Mouse
    Sample Types: Tissue Homogenates
  39. CXCR3-dependent recruitment of antigen-specific T lymphocytes to the liver during murine cytomegalovirus infection.
    Authors: Hokeness KL, Deweerd ES, Munks MW, Lewis CA, Gladue RP, Salazar-Mather TP
    J. Virol., 2007;81(3):1241-50.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. CXCR3 and IFN protein-10 in Pneumocystis pneumonia.
    Authors: McAllister F, Ruan S, Steele C, Zheng M, McKinley L, Ulrich L, Marrero L, Shellito JE, Kolls JK
    J. Immunol., 2006;177(3):1846-54.
    Species: Mouse
    Sample Types: Tissue Homogenates
  41. Mucosal administration of CpG oligodeoxynucleotide elicits strong CC and CXC chemokine responses in the vagina and serves as a potent Th1-tilting adjuvant for recombinant gD2 protein vaccination against genital herpes.
    Authors: Tengvall S, Lundqvist A, Eisenberg RJ, Cohen GH, Harandi AM
    J. Virol., 2006;80(11):5283-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
  42. MyD88-dependent and -independent murine cytomegalovirus sensing for IFN-alpha release and initiation of immune responses in vivo.
    Authors: Delale T, Paquin A, Asselin-Paturel C, Dalod M, Brizard G, Bates EE, Kastner P, Chan S, Akira S, Vicari A, Biron CA, Trinchieri G, Briere F
    J. Immunol., 2005;175(10):6723-32.
    Species: Mouse
    Sample Types: Serum
  43. Orally administered CpG oligodeoxynucleotide induces production of CXC and CC chemokines in the gastric mucosa and suppresses bacterial colonization in a mouse model of Helicobacter pylori infection.
    Authors: Raghavan S, Nystrom J, Fredriksson M, Holmgren J, Harandi AM
    Infect. Immun., 2003;71(12):7014-22.
    Species: Mouse
    Sample Types: Serum

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Reviews for Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA

Average Rating: 4.3 (Based on 3 Reviews)

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Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
By Anonymous on 10/16/2016
Sample Tested: Bone marrow derived macrophages- supernatants,Cell culture supernatant

The protocol was clear to follow and all the reagents were marked, but I wish the resuspension volumes were on the protocol sheet and not the Certificate of Analysis.


Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA
By Anonymous on 10/06/2016
Sample Tested: Cell culture supernatant

Mouse CXCL10/IP-10/CRG-2 DuoSet ELISA, 15 Plate
By Anonymous on 10/26/2015
Sample Tested: Purified murine IP-10 protein

50ul of murine IL-10 Capture antibody (2ug/mL) was coated per well of a 96 well plate at 4 degrees overnight. Purified mIP-10 standard was diluted (7 fold) and assayed to ensure the quality of the ELISA. Mouse IP-10 Detector antibody was added (2h @ RT) followed by a 20 min incubation with Streptavidin-HRP. Substrate solution was added to measure mIP-10 concentration according to the manufactures protocol. The standard curve looked great as can be seen by calculation of the R- squared value. Highly recommend doing ELISA's using kits from R&D.
Buffer: 7-point standard curve