Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit

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(35 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    4.2 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse IP-10
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IP-10 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IP-10 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IP-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IP-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IP-10.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean106217836107250874
Standard Deviation4.21050112067
CV%44.6610.387.7

Recovery

The recovery of mouse IP-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 97 88-104
Heparin Plasma (n=6) 109 93-118
Serum (n=5) 97 81-118
Linearity
To assess the linearity of the assay, four samples containing and/or spiked with high concentrations of mouse IP-10 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CXCL10/IP-10/CRG-2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL10/IP-10/CRG-2
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
    • Entrez Gene IDs
      3627 (Human); 15945 (Mouse);
    • Alternate Names
      C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    35 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas
      Authors: BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu
      J. Immunol, 2017;0(0):.
      Species: Mouse
      Sample Type: Tissue Homogenates
    2. An RNA Molecule Derived From Sendai Virus DI Particles Induces Antitumor Immunity and Cancer Cell-selective Apoptosis.
      Authors: Liu L, Nishikawa T, Kaneda Y
      Mol Ther, 2016;24(1):135-45.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    3. IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
      Authors: Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F
      PLoS Pathog, 2015;11(2):e1004607.
      Species: Mouse
      Sample Type: Serum
    4. Antigen expression determines adenoviral vaccine potency independent of IFN and STING signaling.
      Authors: Quinn, Kylie M, Zak, Daniel E, Costa, Andreia, Yamamoto, Ayako, Kastenmuller, Kathrin, Hill, Brenna J, Lynn, Geoffrey, Darrah, Patricia, Lindsay, Ross W B, Wang, Lingshu, Cheng, Cheng, Nicosia, Alfredo, Folgori, Antonell, Colloca, Stefano, Cortese, Riccardo, Gostick, Emma, Price, David A, Gall, Jason G, Roederer, Mario, Aderem, Alan, Seder, Robert A
      J Clin Invest, 2015;125(3):1129-46.
      Species: Mouse
      Sample Type: Serum
    5. CXCR3 promotes plaque formation and behavioral deficits in an Alzheimer's disease model.
      Authors: Krauthausen M, Kummer M, Zimmermann J, Reyes-Irisarri E, Terwel D, Bulic B, Heneka M, Muller M
      J Clin Invest, 2015;125(1):365-78.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. Activation of A1-adenosine receptors promotes leukocyte recruitment to the lung and attenuates acute lung injury in mice infected with influenza A/WSN/33 (H1N1) virus.
      Authors: Aeffner F, Woods P, Davis I
      J Virol, 2014;88(17):10214-27.
      Species: Mouse
      Sample Type: BALF
    7. Interferon gamma-induced intratumoral expression of CXCL9 alters the local distribution of T cells following immunotherapy with
      Authors: Guirnalda P, Wood L, Goenka R, Crespo J, Paterson Y
      Oncoimmunology, 2013;2(8):e25752.
      Species: Mouse
      Sample Type: Tissue Homogenates
    8. Phenylmethimazole suppresses dsRNA-induced cytotoxicity and inflammatory cytokines in murine pancreatic beta cells and blocks viral acceleration of type 1 diabetes in NOD mice.
      Authors: McCall K, Schmerr M, Thuma J, James C, Courreges M, Benencia F, Malgor R, Schwartz F
      Molecules, 2013;18(4):3841-58.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
      Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
      Am. J. Pathol., 2012;179(1):302-14.
      Species: Mouse
      Sample Type: Tissue Homogenates
    10. Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
      Authors: Gottfries J, Melgar S, Michaelsson E
      PLoS ONE, 2012;7(1):e30005.
      Species: Mouse
      Sample Type: Tissue Homogenates
    11. Deregulation of type I IFN-dependent genes correlates with increased susceptibility to cytomegalovirus acute infection of dicer mutant mice.
      Authors: Ostermann E, Tuddenham L, Macquin C, Alsaleh G, Schreiber-Becker J, Tanguy M, Bahram S, Pfeffer S, Georgel P
      PLoS ONE, 2012;7(8):e43744.
      Species: Mouse
      Sample Type: Serum
    12. Cutting edge: Divergent cell-specific functions of MyD88 for inflammatory responses and organ injury in septic peritonitis.
      Authors: Gais P, Reim D, Jusek G, Rossmann-Bloeck T, Weighardt H, Pfeffer K, Altmayr F, Janssen KP, Holzmann B
      J. Immunol., 2012;188(12):5833-7.
      Species: Mouse
      Sample Type: Plasma
    13. Improved host defense against septic peritonitis in mice lacking MyD88 and TRIF is linked to a normal interferon response.
      Authors: Reim D, Rossmann-Bloeck T, Jusek G, Prazeres da Costa O, Holzmann B
      J. Leukoc. Biol., 2011;90(3):613-20.
      Species: Mouse
      Sample Type: Plasma
    14. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
      Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
      J. Clin. Invest., 2011;121(10):3846-59.
      Species: Mouse
      Sample Type: Tissue Homogenates
    15. Chemokine receptor CXCR3 promotes growth of glioma.
      Authors: Liu C, Luo D, Reynolds BA
      Carcinogenesis, 2011;32(2):129-37.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    16. Quantitative analysis of the secretome of TGF-beta signaling-deficient mammary fibroblasts.
      Authors: Xu BJ, Yan W, Jovanovic B
      Proteomics, 2010;10(13):2458-70.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    17. NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway.
      Authors: Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W
      J. Clin. Invest., 2010;120(5):1645-62.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    18. NKT cells mediate pulmonary inflammation and dysfunction in murine sickle cell disease through production of IFN-gamma and CXCR3 chemokines.
      Authors: Wallace KL, Marshall MA, Ramos SI, Lannigan JA, Field JJ, Strieter RM, Linden J
      Blood, 2009;114(3):667-76.
      Species: Mouse
      Sample Type: Tissue Homogenates
    19. A lymphotoxin-driven pathway to hepatocellular carcinoma.
      Authors: Haybaeck J, Zeller N, Wolf MJ, Weber A, Wagner U, Kurrer MO, Bremer J, Iezzi G, Graf R, Clavien PA, Thimme R, Blum H, Nedospasov SA, Zatloukal K, Ramzan M, Ciesek S, Pietschmann T, Marche PN, Karin M, Kopf M, Browning JL, Aguzzi A, Heikenwalder M
      Cancer Cell, 2009;16(4):295-308.
      Species: Mouse
      Sample Type: Tissue Homogenates
    20. Mouse models of rhinovirus-induced disease and exacerbation of allergic airway inflammation.
      Authors: Bartlett NW, Walton RP, Edwards MR, Aniscenko J, Caramori G, Zhu J, Glanville N, Choy KJ, Jourdan P, Burnet J, Tuthill TJ, Pedrick MS, Hurle MJ, Plumpton C, Sharp NA, Bussell JN, Swallow DM, Schwarze J, Guy B, Almond JW, Jeffery PK, Lloyd CM, Papi A, Killington RA, Rowlands DJ, Blair ED, Clarke NJ, Johnston SL
      Nat. Med., 2008;14(2):199-204.
      Species: Mouse
      Sample Type: BALF
    21. Chemokine receptor CXCR3 and its ligands CXCL9 and CXCL10 are required for the development of murine cerebral malaria.
      Authors: Campanella GS, Tager AM, El Khoury JK, Thomas SY, Abrazinski TA, Manice LA, Colvin RA, Luster AD
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(12):4814-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    22. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
      Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
      Am. J. Pathol., 2008;172(5):1209-21.
      Species: Mouse
      Sample Type: Tissue Homogenates
    23. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
      Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
      Am. J. Pathol., 2007;170(4):1152-64.
      Species: Mouse
      Sample Type: Tissue Homogenates
    24. Regulated expression of the interferon-beta gene in mice.
      Authors: Harkins RN, Szymanski P, Petry H, Brooks A, Qian HS, Schaefer C, Kretschmer PJ, Orme A, Wang P, Rubanyi GM, Hermiston TW
      Gene Ther., 2007;15(1):1-11.
      Species: Mouse
      Sample Type: Serum
    25. A new murine model for bronchiolitis obliterans post-bone marrow transplant.
      Authors: Panoskaltsis-Mortari A, Tram KV, Price AP, Wendt CH, Blazar BR
      Am. J. Respir. Crit. Care Med., 2007;176(7):713-23.
      Species: Mouse
      Sample Type: Serum
    26. CXCL10 is the key ligand for CXCR3 on CD8+ effector T cells involved in immune surveillance of the lymphocytic choriomeningitis virus-infected central nervous system.
      Authors: Christensen JE, de Lemos C, Moos T, Christensen JP, Thomsen AR
      J. Immunol., 2006;176(7):4235-43.
      Species: Mouse
      Sample Type: CSF
    27. Intact TRL 9 and type I interferon signaling pathways are required to augment HSV-1 induced corneal CXCL9 and CXCL10.
      Authors: Wuest T, Austin BA, Uematsu S, Thapa M, Akira S, Carr DJ
      J. Neuroimmunol., 2006;179(1):46-52.
      Species: Mouse
      Sample Type: Tissue Homogenates
    28. Type I IFN modulates host defense and late hyperinflammation in septic peritonitis.
      Authors: Weighardt H, Kaiser-Moore S, Schlautkotter S, Rossmann-Bloeck T, Schleicher U, Bogdan C, Holzmann B
      J. Immunol., 2006;177(8):5623-30.
      Species: Mouse
      Sample Type: Peritoneal Lavage
    29. Local production of chemokines and prostaglandin E2 in the acute, chronic and recovery phase of murine experimental colitis.
      Authors: Drmotova M, Rehnstrom E, Jansson L
      Cytokine, 2006;35(5):275-83.
      Species: Mouse
      Sample Type: Tissue Homogenates
    30. Organ-specific role of MyD88 for gene regulation during polymicrobial peritonitis.
      Authors: Weighardt H, Mages J, Jusek G, Kaiser-Moore S, Lang R, Holzmann B
      Infect. Immun., 2006;74(6):3618-32.
      Species: Mouse
      Sample Type: Tissue Homogenates
    31. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
      Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
      Biochem. Biophys. Res. Commun., 2006;345(3):1122-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    32. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
      Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
      Blood, 2005;107(5):2112-22.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    33. Transcription regulation of TNF-alpha-early response genes by poly(ADP-ribose) polymerase-1 in murine heart endothelial cells.
      Authors: Carrillo A, Monreal Y, Ramirez P, Marin L, Parrilla P, Oliver FJ, Yelamos J
      Nucleic Acids Res., 2004;32(2):757-66.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    34. Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease.
      Authors: Tanaka M, Nakae S, Terry RD, Mokhtari GK, Gunawan F, Balsam LB, Kaneda H, Kofidis T, Tsao PS, Robbins RC
      Blood, 2004;104(12):3789-96.
      Species: Mouse
      Sample Type: Tissue Homogenates
    35. Blockade of CXCR3 receptor:ligand interactions reduces leukocyte recruitment to the lung and the severity of experimental idiopathic pneumonia syndrome.
      Authors: Hildebrandt GC, Corrion LA, Olkiewicz KM, Lu B, Lowler K, Duffner UA, Moore BB, Kuziel WA, Liu C, Cooke KR
      J. Immunol., 2004;173(3):2050-9.
      Species: Mouse
      Sample Type: Serum
    Expand to show all 35 Citations

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