Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

R&D Systems | Catalog # MCX120

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (10 µL), Serum (50 µL)

Sensitivity

0.069 ng/mL

Assay Range

0.156-10 ng/mL (Cell Culture Supernates, Serum)
View all CXCL12/SDF-1 alpha ELISA Kits »

Product Summary for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

The Quantikine Mouse SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse SDF-1 alpha in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant mouse SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SDF-1 alpha.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse SDF-1 alpha

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.09 2.60 4.10 1.25 3.15 4.91
Standard Deviation 0.04 0.12 0.21 0.09 0.24 0.37
CV% 3.7 4.6 5.1 7.2 7.6 7.5

Recovery for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

The recovery of mouse SDF-1 alpha spiked to three different levels in samples throughout the range of the assay was assessed.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 98-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Mouse CXCL12/SDF-1 alpha ELISA Linearity

Scientific Data Images for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

Mouse CXCL12/SDF-1 alpha ELISA Standard Curve

Mouse CXCL12/SDF-1 alpha ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL12/SDF-1 alpha

CXCL12 is a heparin-binding chemokine that regulates lymphopoiesis, enhances the survival of myeloid progenitor cells, regulates the patterning and cell number of neural progenitors, and promotes angiogenesis. CXCL12/SDF-1 alpha is one of several SDF-1 isoforms, all of which can undergo proteolytic processing of the first two N-terminal amino acids by DPPIV/CD26. SDF-1 isoforms signal through CXCR4 and CXCR7 and can also bind Syndecan-4. Their binding to CXCR4 may block the cellular entry of HIV.

 

Alternate Names

SDF1 alpha, SDF1a

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline)

Gene Symbol

CXCL12

Additional CXCL12/SDF-1 alpha Products

Product Documents for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

Customer Reviews for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit (11)

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  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Mouse lymphocytes
    Verified Customer | Posted 09/24/2023
    We isolated mouse tumor tissues and collected serum samples to explore changes in the immune microenvironment of the TME and changes in immune-related indicators in the blood system.
    Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Ricardo Malvicini
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 06/28/2022
    Cell culture supernatant from RAW2647 cells stimulated with LPS and LPS+MSC-EV
    Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: mouse cell line conditioned media
    Verified Customer | Posted 08/31/2021
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Human cell conditioned medium
    Verified Customer | Posted 03/06/2021
    Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: jayeeta giri
    Sample Tested: Cell Lysates
    Verified Customer | Posted 07/08/2019
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: mouse serum
    Verified Customer | Posted 03/08/2019
    Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: 4T1.2 mouse mammary carcinoma cells
    Verified Customer | Posted 04/30/2018
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Pancreatic cancer cells
    Verified Customer | Posted 04/17/2018
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma and Bone marrow supernatant
    Verified Customer | Posted 08/08/2016
    We have used this kit to check SDF gradient in mouse plasma and Bone marrow supernatant. Our results indicated that SDF gradient was shifted in favor of Bone marrow after treatment.
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Mouse plasma and Mouse bone marrow supernatant
    Verified Customer | Posted 08/05/2016
    Very good precision and results are reliable. Published some data already and going to publish more.
    Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
  • Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
    Name: Adam Guess
    Sample Tested: Bone marrow cells
    Verified Customer | Posted 07/12/2016
    Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120

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Protocols

View specific protocols for Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit (MCX120):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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