Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit

(33 citations)
(3 Reviews)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (50 uL)
  • Sensitivity
    0.069 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse SDF-1 alpha
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse SDF-1 alpha in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant mouse SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse SDF-1 alpha.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.


The recovery of mouse SDF-1 alpha spiked to three different levels in samples throughout the range of the assay was assessed.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 98-104
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL12/SDF-1 alpha
CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.
    • Entrez Gene IDs
      6387 (Human); 20315 (Mouse); 493806 (Feline);
    • Alternate Names
      CXCL12 chemokine (C-X-C motif) ligand 12; IRH; PBSF; SCYB12; SDF1 alpha; SDF1; SDF1a; SDF1B; TLSF; TPAR1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 33
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    Sample Type
    1. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
      Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
      Stem Cells Dev, 2015;24(9):1073-81.
      Species: Mouse
      Sample Type: Serum
    2. Direct Renin Inhibition with Aliskiren Improves Ischemia-Induced Neovasculogenesis in Diabetic Animals via the SDF-1 Related Mechanism.
      Authors: Chang T, Wu T, Huang P, Lin C, Chen J, Lin L, Lin S, Chen J
      PLoS ONE, 2015;10(8):e0136627.
      Species: Mouse
      Sample Type: Plasma
    3. Intra-Arterial Transplantation of Allogeneic Mesenchymal Stem Cells Mounts Neuroprotective Effects in a Transient Ischemic Stroke Model in Rats: Analyses of Therapeutic Time Window and Its Mechanisms.
      Authors: Toyoshima A, Yasuhara T, Kameda M, Morimoto J, Takeuchi H, Wang F, Sasaki T, Sasada S, Shinko A, Wakamori T, Okazaki M, Kondo A, Agari T, Borlongan C, Date I
      PLoS ONE, 2015;10(6):e0127302.
      Species: Rat
      Sample Type: Tissue Homogenates
    4. Heparan sulfate inhibits hematopoietic stem and progenitor cell migration and engraftment in mucopolysaccharidosis I.
      Authors: Watson H, Holley R, Langford-Smith K, Wilkinson F, van Kuppevelt T, Wynn R, Wraith J, Merry C, Bigger B
      J Biol Chem, 2014;289(52):36194-203.
      Species: Mouse
      Sample Type: BM soluble fraction
    5. Klotho deficiency disrupts hematopoietic stem cell development and erythropoiesis.
      Authors: Vadakke Madathil S, Coe L, Casu C, Sitara D
      Am J Pathol, 2014;184(3):827-41.
      Species: Mouse
      Sample Type: Serum
    6. The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
      Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
      Blood, 2014;123(7):1032-9.
      Species: Mouse
      Sample Type: cell supernates
    7. CCAAT/enhancer-binding protein beta expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis.
      Authors: Yoshioka S, Miura Y, Yao H, Satake S, Hayashi Y, Tamura A, Hishita T, Ichinohe T, Hirai H, Takaor-Kondo A, Maekawa T
      Stem Cells, 2014;32(3):730-40.
      Species: Mouse
      Sample Type: cell supernates
    8. Resolving cancer-stroma interfacial signalling and interventions with micropatterned tumour-stromal assays.
      Authors: Shen K, Luk S, Hicks D, Elman J, Bohr S, Iwamoto Y, Murray R, Pena K, Wang F, Seker E, Weissleder R, Yarmush M, Toner M, Sgroi D, Parekkadan B
      Nat Commun, 2014;5(0):5662.
      Species: Mouse
      Sample Type: Tissue Lysate
    9. C/EBPbeta mediates osteoclast recruitment by regulating endothelial progenitor cell expression of SDF-1alpha.
      Authors: Fu S, Pang H, Xu J, Wu X
      PLoS ONE, 2014;9(3):e91217.
      Species: Mouse
      Sample Type: cell supernates
    10. Inhibition of the CXCL12/CXCR4-axis as preventive therapy for radiation-induced pulmonary fibrosis.
      Authors: Shu, Hui-Kuo, Yoon, Younghyo, Hong, Samuel, Xu, Kaiming, Gao, Huiying, Hao, Chunhai, Torres-Gonzalez, Edilson, Nayra, Cardenes, Rojas, Mauricio, Shim, Hyunsuk
      PLoS ONE, 2013;8(11):e79768.
      Species: Mouse
      Sample Type: BALF
    11. Protective effects of mesenchymal stem cells with CXCR4 up-regulation in a rat renal transplantation model.
      Authors: Cao, Zhiqiang, Zhang, Geng, Wang, Fuli, Liu, Hongbao, Liu, Long, Han, Yaling, Zhang, Jian, Yuan, Jianlin
      PLoS ONE, 2013;8(12):e82949.
      Species: Mouse
      Sample Type: Homogenized Tissue
    12. Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.
      Authors: Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y
      Br. J. Cancer, 2011;105(10):1615-24.
      Species: Mouse
      Sample Type: Serum
    13. JAM-B regulates maintenance of hematopoietic stem cells in the bone marrow.
      Authors: Arcangeli ML, Frontera V, Bardin F, Obrados E, Adams S, Chabannon C, Schiff C, Mancini SJ, Adams RH, Aurrand-Lions M
      Blood, 2011;118(17):4609-19.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    14. Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence.
      Authors: Oladipupo SS, Hu S, Santeford AC, Yao J, Kovalski JR, Shohet RV, Maslov K, Wang LV, Arbeit JM
      Blood, 2011;117(15):4142-53.
      Species: Human
      Sample Type: Plasma
    15. Application of the chemokine CXCL12 expression plasmid restores wound healing to near normal in a diabetic mouse model.
      Authors: Restivo TE, Mace KA, Harken AH, Young DM
      J Trauma, 2010;69(2):392-8.
      Species: Mouse
      Sample Type: Serum
    16. Novel insight into stem cell mobilization-plasma sphingosine-1-phosphate is a major chemoattractant that directs the egress of hematopoietic stem progenitor cells from the bone marrow and its level in peripheral blood increases during mobilization due to activation of complement cascade/membrane attack complex.
      Authors: Ratajczak MZ, Lee H, Wysoczynski M, Wan W, Marlicz W, Laughlin MJ, Kucia M, Janowska-Wieczorek A, Ratajczak J
      Leukemia, 2010;24(5):976-85.
      Species: Mouse
      Sample Type: Plasma
    17. CXCR4 and CXCR7 regulate angiogenesis and CT26.WT tumor growth independent from SDF-1.
      Authors: Kollmar O, Rupertus K, Scheuer C, Nickels RM, Haberl GC, Tilton B, Menger MD, Schilling MK
      Int. J. Cancer, 2010;126(6):1302-15.
      Species: Mouse
      Sample Type: Serum
    18. Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice.
      Authors: Schroeter MR, Humboldt T, Schafer K, Konstantinides S
      Atherosclerosis, 2009;205(1):63-73.
      Species: Mouse
      Sample Type: Plasma
    19. Synergy between CD26/DPP-IV inhibition and G-CSF improves cardiac function after acute myocardial infarction.
      Authors: Zaruba MM, Theiss HD, Vallaster M, Mehl U, Brunner S, David R, Fischer R, Krieg L, Hirsch E, Huber B, Nathan P, Israel L, Imhof A, Herbach N, Assmann G, Wanke R, Mueller-Hoecker J, Steinbeck G, Franz WM
      Cell Stem Cell, 2009;4(4):313-23.
      Species: Mouse
      Sample Type: Tissue Homogenates
    20. Endothelial nitric oxide synthase promotes bone marrow stromal cell migration to the ischemic myocardium via upregulation of stromal cell-derived factor-1alpha.
      Authors: Li N, Lu X, Zhao X, Xiang FL, Xenocostas A, Karmazyn M, Feng Q
      Stem Cells, 2009;27(4):961-70.
      Species: Mouse
      Sample Type: Tissue Homogenates
    21. Podocytes produce homeostatic chemokine stromal cell-derived factor-1/CXCL12, which contributes to glomerulosclerosis, podocyte loss and albuminuria in a mouse model of type 2 diabetes.
      Authors: Sayyed SG, Hagele H, Kulkarni OP, Endlich K, Segerer S, Eulberg D, Klussmann S, Anders HJ
      Diabetologia, 2009;52(11):2445-54.
      Species: Mouse
      Sample Type: Plasma
    22. Cell-nonautonomous function of Id1 in the hematopoietic progenitor cell niche.
      Authors: Suh HC, Ji M, Gooya J, Lee M, Klarmann KD, Keller JR
      Blood, 2009;114(6):1186-95.
      Species: Mouse
      Sample Type: Serum
    23. Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1.
      Authors: Lin HH, Chen YH, Chang PF, Lee YT, Yet SF, Chau LY
      J. Mol. Cell. Cardiol., 2008;45(1):44-55.
      Species: Mouse
      Sample Type: Tissue Homogenates
    24. Altered SDF-1/CXCR4 axis in patients with primary myelofibrosis and in the Gata1 low mouse model of the disease.
      Authors: Migliaccio AR, Martelli F, Verrucci M, Migliaccio G, Vannucchi AM, Ni H, Xu M, Jiang Y, Nakamoto B, Papayannopoulou T, Hoffman R
      Exp. Hematol., 2008;36(2):158-71.
      Species: Mouse
      Sample Type: Plasma
    25. Rapid chemotherapy-induced acute endothelial progenitor cell mobilization: implications for antiangiogenic drugs as chemosensitizing agents.
      Authors: Shaked Y, Henke E, Roodhart JM, Mancuso P, Langenberg MH, Colleoni M, Daenen LG, Man S, Xu P, Emmenegger U, Tang T, Zhu Z, Witte L, Strieter RM, Bertolini F, Voest EE, Benezra R, Kerbel RS
      Cancer Cell, 2008;14(3):263-73.
      Species: Mouse
      Sample Type: Plasma
    26. The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells.
      Authors: Feng W, McCabe NP, Mahabeleshwar GH, Somanath PR, Phillips DR, Byzova TV
      J. Cell Biol., 2008;183(6):1145-57.
      Species: Mouse
      Sample Type: Plasma
    27. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
      Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
      Eur. J. Pharmacol., 2008;590(1):400-8.
      Species: Mouse
      Sample Type: Tissue Homogenates
    28. Pulmonary stromal-derived factor-1 expression and effect on neutrophil recruitment during acute lung injury.
      Authors: Petty JM, Sueblinvong V, Lenox CC, Jones CC, Cosgrove GP, Cool CD, Rai PR, Brown KK, Weiss DJ, Poynter ME, Suratt BT
      J. Immunol., 2007;178(12):8148-57.
      Species: Mouse
      Sample Type: BALF
    29. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
      Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
      Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
      Species: Mouse
      Sample Type: Plasma
    30. Hypoxia-induced mitogenic factor has proangiogenic and proinflammatory effects in the lung via VEGF and VEGF receptor-2.
      Authors: Yamaji-Kegan K, Su Q, Angelini DJ, Champion HC, Johns RA
      Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(6):L1159-68.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    31. Enalapril increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system.
      Authors: Wang CH, Verma S, Hsieh IC, Chen YJ, Kuo LT, Yang NI, Wang SY, Wu MY, Hsu CM, Cheng CW, Cherng WJ
      J. Mol. Cell. Cardiol., 2006;41(1):34-43.
      Species: Mouse
      Sample Type: Plasma
    32. Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
      Authors: Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
      Blood, 2005;105(3):931-9.
      Species: Mouse
      Sample Type: Peritoneal Lavage
    33. Recruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of kit-ligand.
      Authors: Heissig B, Hattori K, Dias S, Friedrich M, Ferris B, Hackett NR, Crystal RG, Besmer P, Lyden D, Moore MA, Werb Z, Rafii S
      Cell, 2002;109(5):625-37.
      Species: Mouse
      Sample Type: Plasma
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    Average Rating: 4.3 (Based on 3 reviews)

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    Images Ratings Applications Species Reviewed By Date Details
      Very Good
      Anonymous 08/08/2016


    Sample TestedBone marrow supernatant,Plasma

    Other Experimental Details

    Other Experimental DetailsWe have used this kit to check SDF gradient in mouse plasma and Bone marrow supernatant. Our results indicated that SDF gradient was shifted in favor of Bone marrow after treatment.
     Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
      Anonymous 08/05/2016
    Image Details
     Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
    : Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit [MCX120]


    Sample TestedMouse bone marrow supernatant,Mouse plasma

    Other Experimental Details

    Other Experimental DetailsVery good precision and results are reliable. Published some data already and going to publish more.
     Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
    Very Good
      Anonymous 07/12/2016
    Image Details
     Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit MCX120
    : Mouse CXCL12/SDF-1 alpha Quantikine ELISA Kit [MCX120]


    Sample TestedBone marrow cells

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