Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit

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Mouse CXCL13/BLC/BCA-1 ELISA Standard Curve
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Product Details
Citations (15)
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Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (13 uL), EDTA Plasma (13 uL), Heparin Plasma (13 uL)
2.84 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Natural and recombinant mouse BLC/BCA-1
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse BLC/BCA-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse BLC/BCA-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse BLC/BCA-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BLC/BCA-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse BLC/BCA-1.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 51 109 408 52 104 366
Standard Deviation 3.1 3.8 15.4 5.1 7.1 27.1
CV% 6.1 3.5 3.8 9.8 6.8 7.4


The recovery of mouse BLC/BCA-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 93 83-109
EDTA Plasma (n=7) 98 80-115
Heparin Plasma (n=4) 96 80-110
Serum (n=7) 104 85-119


To assess the linearity of the assay, samples spiked with high concentrations of mouse BLC/BCA-1 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CXCL13/BLC/BCA-1 ELISA Linearity

Scientific Data

Mouse CXCL13/BLC/BCA-1 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL13/BLC/BCA-1

CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naïve B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer’s patch development.

Entrez Gene IDs:
10563 (Human); 55985 (Mouse)
Alternate Names:
ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse CXCL13/BLC/BCA-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Radiofrequency Ablation Remodels the Tumor Microenvironment and Promotes Neutrophil-Mediated Abscopal Immunomodulation in Pancreatic Cancer
    Authors: EY Faraoni, BJ O'Brien, LN Strickland, BK Osborn, V Mota, J Chaney, CL Atkins, P Cen, J Rowe, J Cardenas, KL Poulsen, CJ Wray, NC Thosani, JM Bailey-Lun
    Cancer Immunology Research, 2023-01-03;11(1):4-12.
    Species: Mouse
    Sample Types: Serum
  2. Glutathione-dependent redox balance characterizes the distinct metabolic properties of follicular and marginal zone B cells
    Authors: DG Franchina, H Kurniawan, M Grusdat, C Binsfeld, L Guerra, L Bonetti, L Soriano-Ba, A Ewen, T Kobayashi, S Farinelle, AR Minafra, N Vandamme, A Carpentier, FK Borgmann, C Jäger, Y Chen, M Kleinewiet, V Vasiliou, M Mittelbron, K Hiller, PA Lang, D Brenner
    Nature Communications, 2022-04-04;13(1):1789.
    Species: Mouse
    Sample Types: Serum
  3. Gas6 induces inflammation and reduces plaque burden but worsens behavior in a sex-dependent manner in the APP/PS1 model of Alzheimer's disease
    Authors: LD Owlett, B Karaahmet, L Le, EK Belcher, D Dionisio-S, JA Olschowka, MR Elliott, MK O'Banion
    Journal of Neuroinflammation, 2022-02-07;19(1):38.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. The Nutritional Supplement L-Alpha Glycerylphosphorylcholine Promotes Atherosclerosis
    Authors: Z Wang, J Hazen, X Jia, E Org, Y Zhao, LJ Osborn, N Nimer, J Buffa, MK Culley, D Krajcik, BH van den Bo, K Zwinderman, BS Levison, M Nieuwdorp, AJ Lusis, JA DiDonato, SL Hazen
    International Journal of Molecular Sciences, 2021-12-15;22(24):.
    Species: Mouse
    Sample Types: Plasma
  5. Suppressing neutrophil-dependent angiogenesis abrogates resistance to anti-VEGF antibody in a genetic model of colorectal cancer
    Authors: Y Itatani, T Yamamoto, C Zhong, AA Molinolo, J Ruppel, P Hegde, MM Taketo, N Ferrara
    Proc. Natl. Acad. Sci. U.S.A., 2020-08-19;0(0):.
    Species: Mouse
    Sample Types: Serum
  6. Dual-mechanism based CTLs infiltration enhancement initiated by Nano-sapper potentiates immunotherapy against immune-excluded tumors
    Authors: Y Huang, Y Chen, S Zhou, L Chen, J Wang, Y Pei, M Xu, J Feng, T Jiang, K Liang, S Liu, Q Song, G Jiang, X Gu, Q Zhang, X Gao, J Chen
    Nat Commun, 2020-01-30;11(1):622.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. GM-CSF Promotes Chronic Disability in Experimental Autoimmune Encephalomyelitis by Altering the Composition of Central Nervous System-Infiltrating Cells, but Is Dispensable for Disease Induction
    Authors: PC Duncker, JS Stoolman, AK Huber, BM Segal
    J. Immunol., 2017-12-29;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy
    J Neuroinflammation, 2016-11-28;13(1):298.
    Species: Mouse
    Sample Types: Serum
  9. Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia
    Cancer Discov, 2015-12-29;6(3):256-69.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Prion infection of mouse brain reveals multiple new upregulated genes involved in neuroinflammation or signal transduction.
    Authors: Carroll J, Striebel J, Race B, Phillips K, Chesebro B
    J Virol, 2014-12-10;89(4):2388-404.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Repetitive hypoxic preconditioning induces an immunosuppressed B cell phenotype during endogenous protection from stroke.
    Authors: Monson N, Ortega S, Ireland S, Meeuwissen A, Chen D, Plautz E, Shubel E, Kong X, Li M, Freriks L, Stowe A
    J Neuroinflammation, 2014-01-31;11(0):22.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice.
    Authors: Grabner R, Lotzer K, Dopping S, Hildner M, Radke D, Beer M, Spanbroek R, Lippert B, Reardon CA, Getz GS, Fu YX, Hehlgans T, Mebius RE, van der Wall M, Kruspe D, Englert C, Lovas A, Hu D, Randolph GJ, Weih F, Habenicht AJ
    J. Exp. Med., 2009-01-12;206(1):233-48.
    Species: Mouse
    Sample Types: Serum
  13. Cyclooxygenase inhibition during allergic sensitization increases STAT6-independent primary and memory Th2 responses.
    Authors: Zhou W, Newcomb DC, Moore ML, Goleniewska K, O'Neal JF, Peebles RS
    J. Immunol., 2008-10-15;181(8):5360-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
    Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
    Am. J. Pathol., 2007-04-01;170(4):1152-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
    Authors: Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
    J. Immunol., 2006-11-15;177(10):7296-302.
    Species: Mouse
    Sample Types: Cell Culture Supernates


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