Mouse CXCL17/VCC-1 Antibody

Catalog # Availability Size / Price Qty
MAB4270
MAB4270-SP
Mouse CXCL17/VCC-1 Antibody in Data
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Mouse CXCL17/VCC-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant mouse (rm) CXCL17/VCC‑1 in direct ELISAs and Western blots. In direct ELISAs, 50% cross‑reactivity with recombinant human CXCL17 is observed/VCC-1.
Source
Monoclonal Rat IgG2B Clone # 510614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CXCL17/VCC‑1
Ser31-Leu119
Accession # Q5UW37
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse CXCL17 by Western Blot. View Larger

Detection of Mouse CXCL17 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse CXCL17 Monoclonal Antibody (Catalog # MAB4270) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CXCL17 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 (with 3% BSA instead of 5% milk in the Antibody Solution).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL17/VCC-1

CXCL17, also known as dendritic cell and monocyte chemokine-like protein (DMC) and VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation sites, so cleavage of a 22 amino acid (aa) signal sequence likely results in a mature mouse CXCL17 molecule of 97 aa and 11 kDa size. CXCL17 is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS‑activated peripheral blood monocytes and dendritic cells, and also binds these cells specifically (1). CXCL17 expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes upregulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). CXCL17 and two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show significantly correlated expression with that of VEGF in primary lung, breast and esophageal tumors (2). CXCL17 is therefore suggested to play a role in tumor angiogenesis. Mature mouse CXCL17 shares 82%, 71% and 66% amino acid sequence identity with rat, human and bovine CXCL17, respectively.

References
  1. Pisabarro, M. T. et al. (2006) J. Immunol. 176:2069.
  2. Weinstein, E. J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.
Entrez Gene IDs
284340 (Human); 232983 (Mouse)
Alternate Names
Chemokine (C-X-C Motif) Ligand 17; C-X-C Motif Chemokine 17; CXCL17; Dcip1; Dendritic Cell And Monocyte Chemokine-Like Protein; DMC; UNQ473; VCC1; VCC-1; VEGF Coregulated Chemokine 1; VEGF Co-Regulated Chemokine 1

Product Datasheets

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