Mouse CXCL2/MIP-2 Antibody

Catalog # Availability Size / Price Qty
Chemotaxis Induced by CXCL2/MIP-2 and Neutral-ization by Mouse CXCL2/ MIP-2 Antibody.
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Product Details
Citations (20)
Supplemental Products

Mouse CXCL2/MIP-2 Antibody Summary

Species Reactivity
Detects mouse CXCL2/MIP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) GRO beta, recombinant mouse (rm) GRO gamma, rmKC and rmLIX is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse CXCL2/MIP-2
Accession # P10889
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
5-15 µg/mL
Perfusion fixed frozen sections of mouse thymus
5-15 µg/mL
See below
Measured by its ability to neutralize CXCL2/MIP-2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.015-0.075 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/MIP-2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CXCL2/MIP-2 and Neutral-ization by Mouse CXCL2/ MIP-2 Antibody. View Larger

Chemotaxis Induced by CXCL2/MIP-2 and Neutral-ization by Mouse CXCL2/ MIP-2 Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND50 is typically 0.015-0.075 µg/mL.

Immunocytochemistry CXCL2/MIP-2 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CXCL2/MIP‑2 in Mouse Splenocytes. CXCL2/MIP-2 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL2/GRO beta/MIP-2/CINC-3

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Entrez Gene IDs
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

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Citations for Mouse CXCL2/MIP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex
    Authors: X Dong, Q Zhang, X Yu, D Wang, J Ma, J Ma, SH Shi
    Nature Neuroscience, 2022;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Role of Innate Inflammation in the Regulation of Tissue Remodeling during Tooth Eruption
    Authors: Y Makino, K Fujikawa, M Matsuki-Fu, S Inoue, M Nakamura
    Dentistry journal, 2021;9(1):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis
    Authors: MH Mursalin, PS Coburn, FC Miller, ET Livingston, R Astley, MC Callegan
    Investigative Ophthalmology & Visual Science, 2021;62(14):14.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Tumor-Associated Microglia/Macrophages as a Predictor for Survival in Glioblastoma and Temozolomide-Induced Changes in CXCR2 Signaling with New Resistance Overcoming Strategy by Combination Therapy
    Authors: RM Urbantat, C Jelgersma, S Brandenbur, M Nieminen-K, I Kremenetsk, J Zollfrank, S Mueller, K Rubarth, A Koch, P Vajkoczy, G Acker
    International Journal of Molecular Sciences, 2021;22(20):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice
    Authors: K Nakajima, M Ono, U Radovi?, S Dizdarevi?, SI Tomizawa, K Kuroha, G Naganatsu, I Hoshi, R Matsunaga, T Shirakawa, T Kurosawa, Y Miyazaki, M Seki, Y Suzuki, H Koseki, M Nakamura, T Suda, K Ohbo
    Dis Model Mech, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Mannan-binding lectin deficiency exacerbates sterile liver injury in mice through enhancing hepatic neutrophil recruitment
    Authors: J Zhou, J Li, Y Yu, Y Liu, H Li, Y Liu, J Wang, L Zhang, X Lu, Z Chen, D Zuo
    J. Leukoc. Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. An IFN?/CXCL2 regulatory pathway determines lesion localization during EAE
    Authors: JS Stoolman, PC Duncker, AK Huber, DA Giles, JM Washnock-S, AM Soulika, BM Segal
    J Neuroinflammation, 2018;15(1):208.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Novel adherent CD11b+Gr-1+tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment
    Authors: T Tsubaki, T Kadonosono, S Sakurai, T Shiozawa, T Goto, S Sakai, T Kuchimaru, T Sakamoto, H Watanabe, G Kondoh, S Kizaka-Kon
    Oncotarget, 2018;9(13):11209-11226.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
    Sci Rep, 2016;6(0):37695.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. TIARP attenuates autoantibody-mediated arthritis via the suppression of neutrophil migration by reducing CXCL2/CXCR2 and IL-6 expression
    Sci Rep, 2016;6(0):38684.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The HMGB1/RAGE axis triggers neutrophil-mediated injury amplification following necrosis.
    Authors: Huebener, Peter, Pradere, Jean-Phi, Hernandez, Celine, Gwak, Geum-You, Caviglia, Jorge Ma, Mu, Xueru, Loike, John D, Jenkins, Rosalind, Antoine, Daniel J, Schwabe, Robert F
    J Clin Invest, 2015;125(2):539-50.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
    Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
    J Biol Chem, 2015;290(4):2053-68.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Type I IFN suppresses Cxcr2 driven neutrophil recruitment into the sensory ganglia during viral infection.
    Authors: Stock A, Smith J, Carbone F
    J Exp Med, 2014;211(5):751-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways.
    Authors: Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D
    Am J Pathol, 2014;184(6):1877-89.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
    Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
    Int J Cancer, 2014;134(6):1346-58.
  16. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Initial delay in the immune response to Francisella tularensis is followed by hypercytokinemia characteristic of severe sepsis and correlating with upregulation and release of damage-associated molecular patterns.
    Authors: Mares CA, Ojeda SS, Morris EG, Li Q, Teale JM
    Infect. Immun., 2008;76(7):3001-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  18. The Duffy antigen receptor for chemokines in acute renal failure: A facilitator of renal chemokine presentation.
    Authors: Zarbock A, Schmolke M, Bockhorn SG, Scharte M, Buschmann K, Ley K, Singbartl K
    Crit. Care Med., 2007;35(9):2156-63.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
    Authors: Cardona AE, Gonzalez PA, Teale JM
    Infect. Immun., 2003;71(5):2634-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
    Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K
    J. Clin. Invest., 2001;108(9):1307-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC


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