hh Mouse CXCL2/MIP-2 Antibody AF-452-NA: R&D Systems

Mouse CXCL2/MIP-2 Antibody

(13 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL2/MIP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) GRO alpha, rhGRO beta, rhGRO gamma, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, and rrCINC-3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CXCL2/MIP-2
    Ala28-Asn100
    Accession # P10889
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CXCL2/MIP‑2 (Catalog # 452-M2)
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse thymus
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CXCL2/MIP-2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.015-0.075 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/MIP-2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL2/MIP-2 and Neutral­ization by Mouse CXCL2/
MIP-2 Antibody.
Recombinant Mouse CXCL2/MIP-2 (Catalog # 452‑M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND50 is typically 0.015‑0.075 µg/mL.
Immunocytochemistry
CXCL2/MIP‑2 in Mouse Splenocytes. CXCL2/MIP‑2 was detected in immersion fixed mouse splenocytes stimulated with LPS and monensin using Goat Anti-Mouse CXCL2/MIP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL2/GRO beta/MIP-2/CINC-3

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

  • Entrez Gene IDs:
    2920 (Human); 20310 (Mouse); 114105 (Rat)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
    Sci Rep, 2016;6(0):37695.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  2. TIARP attenuates autoantibody-mediated arthritis via the suppression of neutrophil migration by reducing CXCL2/CXCR2 and IL-6 expression
    Sci Rep, 2016;6(0):38684.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Mycobacterium tuberculosis-triggered Hippo pathway orchestrates CXCL1/2 expression to modulate host immune responses
    Sci Rep, 2016;6(0):37695.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
    Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
    J Biol Chem, 2015;290(4):2053-68.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. The HMGB1/RAGE axis triggers neutrophil-mediated injury amplification following necrosis.
    Authors: Huebener, Peter, Pradere, Jean-Phi, Hernandez, Celine, Gwak, Geum-You, Caviglia, Jorge Ma, Mu, Xueru, Loike, John D, Jenkins, Rosalind, Antoine, Daniel J, Schwabe, Robert F
    J Clin Invest, 2015;125(2):539-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  6. Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways.
    Authors: Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D
    Am J Pathol, 2014;184(6):1877-89.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
    Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
    Int J Cancer, 2014;134(6):1346-58.
  8. Type I IFN suppresses Cxcr2 driven neutrophil recruitment into the sensory ganglia during viral infection.
    Authors: Stock A, Smith J, Carbone F
    J Exp Med, 2014;211(5):751-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  9. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. Initial delay in the immune response to Francisella tularensis is followed by hypercytokinemia characteristic of severe sepsis and correlating with upregulation and release of damage-associated molecular patterns.
    Authors: Mares CA, Ojeda SS, Morris EG, Li Q, Teale JM
    Infect. Immun., 2008;76(7):3001-10.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  11. The Duffy antigen receptor for chemokines in acute renal failure: A facilitator of renal chemokine presentation.
    Authors: Zarbock A, Schmolke M, Bockhorn SG, Scharte M, Buschmann K, Ley K, Singbartl K
    Crit. Care Med., 2007;35(9):2156-63.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  12. CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
    Authors: Cardona AE, Gonzalez PA, Teale JM
    Infect. Immun., 2003;71(5):2634-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
    Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K
    J. Clin. Invest., 2001;108(9):1307-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 13 Citations
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